Advanced in vitro exposure systems.

12. Jun. 2019

Molecular Signature of Asthma-Enhanced Sensitivity to CuO Nanoparticle Aerosols from 3D Cell Model

DOI: 10.1021/acsnano.9b01823

Ingeborg Kooter1, Marit Ilves 2, Mariska Gröllers-Mulderij 1, Evert Duistermaat 3, Peter C. Tromp 1, Frieke Kuper 1, Pia Kinaret 4,5, Kai Savolainen 6, Dario Greco 4,5, Piia Karisola 2, Joseph Ndika 2, and Harri Alenius 2,7

1The Netherlands Organization for Applied Scientific Research, TNO, P.O. Box 80015, Utrecht 3584 CB, The Netherlands
2Human Microbiome Research, Faculty of Medicine, University of Helsinki, P.O. Box 21, Helsinki 00290, Finland
3Triskelion B.V., P.O. Box 844, Zeist 3704 HE, The Netherlands
4Faculty of Medicine and Life Sciences, University of Tampere, Tampere FI-33014, Finland
5Institute of Biotechnology, University of Helsinki, P.O. Box 56, Helsinki 00014, Finland
6Finnish Institute of Occupational Health, P.O. Box 40, Helsinki 00014, Finland
7Institute of Environmental Medicine, Karolinska Institutet, P.O. Box 210, Stockholm SE-17176, Sweden

3D human bronchial epithelial cells were cultured at the air−liquid interface that mimics relevant inhalatory exposure were exposed to aerosols of pristine (nCuO) and carboxylated (nCuOCOOH) copper oxide nanoparticles. This paper shows that the existence of asthma enhances sensitivity of the airways to nanoparticle aerosols, possibly as a combined result of a hyperactive airway and inefficient mucociliary clearance mechanisms in asthmatics. The test results are shown in cell viabilty (LDH), Inflammation (IL6, IL8, MCP1) and Transcroptomics.

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27. May. 2019

Characterization of the Vitrocell® Cloud SEQ 24 System and its Application in Exposure to Aerosolized Bortezomib and Ibuprofen

S. Steiner1, Sandra Ferreira1, Moran Morelli1, Audrey Baldi1, Arkadiusz K. Kuczaj1, Stefan Frentzel1, Marco van der Toorn1, Manuel Peitsch1, Julia Hoeng1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

This poster shows the investigation how aerosols are delivered within the Cloud SEQ 24 and how cell cultures respond to exposures in the system. Ibuprofen and Bortezomib was exposed to three dimensional organotypic tissue cultures of the human bronchial epithelium and biological endpoints were measured: Cytotoxicity, Interleukin 8 and Proteasome activity.

 

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25. May. 2019

In vitro model for the prediction of respiratory sensitization of inhaled chemicals and protein allergens

ISAM, Montreux, 25 – 29 May 2019

Weber P. 1, Chary A.1,2, Saibene M. 1, Serchi T.1, Cambier S.1 , Moschini E.1, Contal S.1, Hennen J.2, Ezendam J.3, Blömeke B.2, Gutleb A.C.1
1 Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Luxembourg. 
2 Department of Environmental Toxicology, Trier University, Germany.
3Centre for Environmental Protection, National Institute for Public Health and the Environment (RIVM), The Netherlands.

The Aim of this study is the adaptation of a 3D-co-culture system previously developed by Klein et al.2013. Inclusion of dendritic cells to study respiratory sensitization processes and identification and evaluation of markers to assess the respiratory sensitizing potential of inhaled compounds.

 

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Categories: Publications

Tags: VITROCELL Cloud

5. Apr. 2019

Assessing the respiratory toxicity of dihydroxyacetone using an in vitro human airway epithelial tissue model

https://doi.org/10.1016/j.tiv.2019.04.007
Yiying Wanga, Qiangen Wub, Levan Muskhelishvilic, Kelly Davisc, Matthew Bryantb, Xuefei Caoa
a Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
b Division of Biochemistry Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
c Toxicologic Pathology Associates, Jefferson, AR 72079, United States of America

Unintentional exposure of respiratory mucosal surfaces to DHA aerosols is highly possible during sunless tanning sessions, a possibility for which it is not approved. In this study, the potential respiratory toxicity of DHA using the ALI airway tissue model is evaluated. To mimic in vivo exposure conditions, a liquid aerosol generation and exposure system was used and exposed ALI cultures to a range of non-cytotoxic doses of DHA aerosols at the air-liquid interface.


 

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22. Mar. 2019

In vitro toxicological evaluation of emissions from catalytic oxidation removal of industrial VOCs by air/liquid interface (ALI) exposure system in repeated mode.

DOI: 10.1016/j.tiv.2019.03.030
Méausoone C1, El Khawaja R1, Tremolet G1, Siffert S1, Cousin R1, Cazier F2, Billet S1, Courcot D3, Landkocz Y1.
1 UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.
2 Centre Commun de Mesure, Université du Littoral Côte d'Opale, Dunkerque, France.
3 UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.. Electronic address: dominique.courcot@univ-littoral.fr.

BEAS-2B cells were exposed using an Air-Liquid Interface (ALI) System (Vitrocell®) to gaseous flows of toluene vapors and outflow after catalytic oxidation of toluene. After exposure to gaseous flow, cytotoxicity, inflammatory response and Xenobiotic Metabolism Enzymes (XME) gene expression were investigated.

Highlights
• Toxicity of toluene increases with time when cells are repeatedly exposed.
• Toxicological analysis helps for validation of catalysts used in treatment of VOCs.
• Cellular response supports the identification of chemically undetected by-products.


 

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6. Mar. 2019

Carbendazim induces death in alveolar epithelial cells: A comparison between submerged and at the air-liquid interface cell culture.

DOI: 10.1016/j.tiv.2019.03.004

Tollstadius BF, da Silva ACG, Oliveira BC, Pedralli, Valadares MC.

Laboratory of Education and Research in In vitro Toxicology, Faculty of Pharmacy, Universidade Federal de Goiás, Goiânia, GO, Brazil.

In this work, the toxicity of the fungicide Carbendazim upon A549 alveolar cells was tested in comparison of monolayer and air-liquid interface cell system exposition. 


 

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5. Mar. 2019

A lower impact of an acute exposure to electronic cigarette aerosols than to cigarette smoke in human organotypic buccal and small airway cultures was demonstrated using systems toxicology assessment

DOI:10.1007/s11739-019-02055-x
Filippo Zanetti1, Athanasios Kondylis1, Florian Martin1, Patrice Leroy1, Shoaib Majeed1, Sandro Steiner1, Yang Xiang1, Laura Ortega Torres1, Keyur Trivedi1, Emmanuel Guedj1, Celine Merg1, Stefan Frentzel1, Nikolai V. Ivanov1, Utkarsh Doshi2, Kyeonghee Monica Lee2, Willie J. McKinneyJr2, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
2 Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

 

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20. Feb. 2019

An in vitro coculture system for the detection of sensitization following aerosol exposure

DOI:10.14573/altex.1901241

Aline Chary1,2, Tommaso Serchi1, Elisa Moschini1, Jennifer Hennen2, Sébastien Cambier1, Janine Ezendam3, Brunhilde Blömeke2 and Arno C. Gutleb1
1Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Luxembourg; 
2Department of Environmental Toxicology, University Trier, Germany; 
3Centre for Health Protection, National Institute for Public Health and the Environment, The Netherlands

 

The Vitrocell® Cloud-6 system was used for the exposure of a coculture in vitro model to test compounds and vehicle controls. The 3D in vitro model was cultured at the air liquid interface consists of A549, EA.hy926, PMA-differentiated THP-1 and non-differentiated THP-1 cells, that were exposed to nebulized chemical respiratory sensitizers. The results are shown in flow cytometry measurements, cytokine release and gene expression.

 

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2. Feb. 2019

Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model.

DOI: 10.1016/j.yrtph.2019.01.036

Czekala L1, Simms L2, Stevenson M2, Tschierske N2, Maione AG3, Walele T2.
1 Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK. Electronic address: Lukasz.Czekala@uk.imptob.com.
2 Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK.
3 MatTek, 200 Homer Ave., Ashland, MA, USA.

In this research article a respiratory epithelial model, EpiAirway, was used to examine the biological effects of nicotine-containing blu PLUS + e-cigarettes, in comparison to conventional cigarette smoke. Tissues were exposed at the air-liquid interface to cigarette smoke or e-cigarette aerosol generated using a VITROCELL VC1 smoking/vaping robot. 

 

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20. Dec. 2018

Establishing the Vitrocell® Powder Chamber as a particle size-selective platform for in vitro dry powder testing

S. Steiner1 , M. Hittinger2 , K. Knoth2 , H. Gross2 , S. Frentzel1 , A. Kuczaj1 , J. Hoeng1 , T. Krebs3 , M. Peitsch1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland
2 PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany
3 Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

The poster shows the use of the Vitrocell® Powder Chamber for dry powder testing by online monitoring of the powder mass deposition, by QCMs Nicotine quantification, particle number deposition and particle size distribution.

 

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