Advanced in vitro exposure systems.

27. Mar. 2020

Reliable in vitro Exposure Systems for Screening of Antiviral Lead Compounds against Coronavirus (COVID-19 / SARS-CoV-2)

VITROCELL Application Note for Highly efficient and realistic application of aerosolized drugs to ­human cells of the respiratory tract under physiologic conditions

In inhalation therapy, drugs are deposited as aerosols on cells of the respiratory tract from the nasal or lung region. Physiologically realistic in vitro cell culture models of the pulmonary epithelium and the air-blood barrier as well as from the nasal region are commercially available. Recently, these models have been refined to mimic  SARS-CoV-2 infections.

VITROCELL Application Note.

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26. Mar. 2020

Screening antiviraler pharmazeutischer Leitwirkstoffe gegen das Coronavirus (COVID-19 / SARS-CoV-2)

VITROCELL in vitro Expositionssysteme für hocheffiziente und realistische Applikation aerosolisierter Medikamente auf menschliche Zellen des Atemtraktes unter physiologischen Bedingungen.

In der Inhalationstherapie werden pharmazeutische Präparate als Aerosole auf Zellen des Atemtraktes im Nasen- oder Lungenbereich appliziert. Zur präklinischen Entwicklung neuer Arzneimittel werden dafür oft kommerziell erhältliche, physiologisch relevante in vitro Zellkulturmodelle des Nasen-, Rachen- oder Lungenepithels verwendet. In jüngster Zeit wurden diese Modelle verfeinert, um SARS-CoV-2 Infektionen nachzuahmen.

VITROCELL Anwendungshinweis.

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21. May. 2020

A novel TEM grid sampler for airborne particles to measure the cell culture surface dose

Sonja Mülhopt1, Christoph Schlager2, Markus Berger2, Sivakumar Murugadoss3, Peter H. Hoet3, Tobias Krebs2, Hanns-Rudolf paur1 & Dieter Stapf1
1Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, Eggenstein-Leopoldshafen, 76344, Germany. 
2Vitrocell Systems GmbH, Waldkirch, 79183, Germany. 
3KU Leuven, Environment and Health, Leuven, 3000, Belgium.

 

The surface dose and the spatial distribution on the membrane delivers important data for  measuring dose-response relationships in toxicity studies.  Image evaluation of transmission electron  microscopy (TEM) samples is a highly sensitive method for determination of deposition. This paper reports  the development and characterization of a novel holder for film coated TEM copper grids, which allows for  sampling under identical geometric and ambient conditions as in a cell culture chamber. 

 

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14. Apr. 2020

Toxicity and Gene Expression Profiling of Copper- and Titanium-Based Nanoparticles Using Air–Liquid Interface Exposure

https://doi.org/10.1021/acs.chemrestox.9b00489


Matthias Hufnagel, Sarah Schoch, Johanna WallBettina, Maria Strauch, Andrea Hartwig
Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany

 

In this study an ALI exposure was combined with a high-throughput RT-qPCR approach to evaluate the toxicological potential nanoparticles in A549 cells.

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26. Mar. 2020

New lung model gives lab animals a breather

A first-of-its-kind 3D lung model could replace animals in inhalation toxicity research.

Autor

Francesca Lake

Source
Barosova H, Maione AG, Septiadi D et al. Use of EpiAlveolar lung model to predict fibrotic potential of multiwalled carbon nanotubes. ACS Nano

doi:10.1021/acsnano.9b06860 (2020);
https://pubs.acs.org/doi/10.1021/acsnano.9b06860
https://www.eurekalert.org/pub_releases/2020-03/pfte-psg032420.php

 

The model utilizes an air–liquid interface exposure device, VITROCELL Cloud, which PETA had awarded to the Heriot–Watt group in 2017 along with three other groups, in an attempt to support the development of non-animal toxicity testing methods.

 

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Categories: Publications

Tags: VITROCELL Cloud

24. Mar. 2020

Comparison of Vapor and Liquid Phase Acrolein Exposures to Air Liquid Interface (ALI) Cell Cultures

David H. Brandwein, F. Adam Bettmann, Michael P. DeLorme, Alan T. Eveland, Lawrence M. Milchak 
3M Corporate Toxicology and Environmental Science, St. Paul, MN
 

The STL is working to develop an in vitro screening ALI model to assess the acute respiratory irritation potential for new chemicals. These experiments examined multiple aspects of the model, including different cell culture systems (A549 and EpiAirway), different exposure methods (dynamic vapor and liquid phase), and different post exposure periods, all using acrolein as a model respiratory irritant. The goal was to better understand the critical parameters of the cell systems and exposure methods to enable the development of a consistent screening model, while gaining clarity of the dosimetry. 

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20. Mar. 2020

Use of EpiAlveolar Lung Model to Predict Fibrotic Potential of Multiwalled Carbon Nanotubes

https://dx.doi.org/10.1021/acsnano.9b06860

Autors
Barosova H1, Maione AG2, Septiadi D1, Sharma M3, Haeni L1, Balog S1, O'Connell O2, Jackson GR2, Brown D4, Clippinger AJ3, Hayden P2,5, Petri-Fink A1,6, Stone V4, Rothen-Rutishauser B1.

1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
2 MatTek Corporation, 200 Homer Avenue, Ashland, Massachusetts 01721, United States.
3 PETA International Science Consortium Ltd., 8 All Saints Street, London N1 9RL, U.K.
4 Nano-Safety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, U.K.
5 BioSurfaces, Inc., 200 Homer Ave, Ashland, Massachusetts 01721, United States.
6 Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

 

This study characterizes a 3D in vitro alveolar tissue model comprised entirely of primary human cells to investigate its ability to predict pulmonary fibrosis. The study demonstrated that the EpiAlveolar model recapitulates relevant lung phenotypes and functions and is stable at VITROCELL Cloud with repeated exposures over 3 weeks. 

 

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25. Feb. 2020

Comparison of in vitro toxicity of aerosolized engineered nanomaterials using air-liquid interface mono-culture and co-culture models

https://doi.org/10.1016/j.impact.2020.100215

Autors

Yifang Wanga, Andrea,Adamcakova-Doddb, Benjamin R.Steinesb, Xuefang Jingb, Aliasger K.Salemc, Peter S.Thorneab
a Human Toxicology Interdisciplinary Program, University of Iowa, Iowa City, IA, USA
b Occupational and Environmental Health, University of Iowa, Iowa City, IA, USA
c College of Pharmacy, University of Iowa, Iowa City, IA, USA

 

Highlights
• An in vitro co-culture model utilizing endothelial and epithelial cells and differentiated macrophages was established.
• Air-liquid interface exposures to Ag-SiO2 and CuO nanoparticles produced cell death, oxidative stress and cytokine release.
• Mono- and co-culture models showed comparable excposure outcomes except that cytokines were higher in the co-culture system.
• Compared to epithelia cells or macrophages alone, responses to CuO in the co-culture model indicated cellular interaction

 

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19. Feb. 2020

Differences in cytotoxicity of lung epithelial cells exposed to titanium dioxide nanofibers and nanoparticles: Comparison of air-liquid interface and submerged cellcultures

doi: 10.1016/j.tiv.2020.104798

Autors

Medina-Reyes EI1, Delgado-Buenrostro NL2, Leseman DL3, Déciga-Alcaraz A2, He R4, Gremmer ER3, Fokkens PHB3, Flores-Flores JO5, Cassee FR4, Chirino YI2.
1 Laboratorio de Carcinogénesis y Toxicología, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Estado de México, Mexico. Electronic address: medinaingrid0@gmail.com.
2 Laboratorio de Carcinogénesis y Toxicología, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Estado de México, Mexico.
3 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
4 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands; Institute of Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands.
5 Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, CP 04510 Ciudad de México, Mexico

 

The Air-liquid interface (ALI) model has emerged as a closer physiological system that mimics exposure in gaseous and liquid phases. This Study shows that the exposure to TiO2 nanofibers and nanoparticles displays similar toxicity both the ALI and submerged cell cultures, using lung epithelial A549 cells. Additionally, they detected for the first time that TiO2 nanofibers were located into the nucleus.

 

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15. Jan. 2020

Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward.

Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)

https://doi.org/10.1016/j.mrgentox.2020.503135

Authors
StefanPfuhlera, Janvan Benthemb, RodgerCurrenc, Shareen H.Doakd, MariaDusinskae, MakotoHayashif, Robert H.Heflichg, DarrenKiddh, DavidKirklandi, YangLuanj, GladysOuedraogok, KerstinReisingerl, ToshioSofunim, Frédériquevan Ackern, YingYango, RaffaellaCorvip
a Procter and Gamble, Mason Business Centre, Mason, OH, USA
b National Institute for Public Health and the Environment, Centre for Health Protection, Bilthoven, the Netherlands
c Institute for In Vitro Sciences, Inc., Gaithersburg, MD, USA
d Swansea University Medical School, Singleton Park, Swansea, SA2 8PP, Wales, UK
e Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute for Air Research, Kjeller, Norway
f makoto international consulting, Ebina, Japan
g U.S. Food and Drug Administration/National Center for Toxicological Research, Jefferson, AR, USA
h Covance Laboratories Ltd, Otley Road, Harrogate, HG3 1PY, UK
i Kirkland Consulting, PO Box 79, Tadcaster, LS24 0AS, UK
j School of Public Health, Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, PR China
k L’Oréal R&I, Aulnay-sous-bois, France
l Henkel AG & Co KGaA, Duesseldorf, Germany
m Formerly National Institute of Health Sciences, Tokyo, Japan
n Triskelion B.V., Zeist, the Netherlands
o Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, PR China
p European Commission, Joint Research Centre (JRC), Ispra, Italy

 

Highlights
• Extensive progress made in development of 3D organ-based genotoxicity assays.
• 3D culture models represent major exposure routes: dermal, oral, inhalation.
• The 3D skin comet and MN assays are considered mature and sufficiently validated.
• Liver and airway model-based genotoxicity assays show promise but are at early stage.<

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