Advanced in vitro exposure systems.

6. Jun. 2018

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

DOI: 10.2217/nnm-2017-0358

Savvina Chortarea1,2, Kleanthis Fytianos1,3,4, Laura Rodriguez-Lorenzo1, Alke Petri-Fink1,5 & Barbara Rothen-Rutishauser1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
2Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
3Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
4Department of Clinical Research, University of Bern, Bern, Switzerland
5Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

 

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1. Jun. 2018

Charakterisierung von Partikelemissionen aus dem Trennschleifprozess von kohlefaserverstärktem Beton (Carbonbeton)

Autoren:
Hillemann, L.; Stintz, M.; Streibel, T.; Zimmermann, R.; Öder, S.; Kasurinen, S.; di Bucchianico, S.; Kanashova, T.; Dittmar, G.; Konzack, D.; Große, S.; Rudolph, A.; Berger, M.; Krebs, T.; Saraji-Bozorgzad, M. R.; Walte, A.

Die Wirkung von Emissionen aus dem Trennen von Carbonbeton werden in einer Kombination aus Trennstaubgenerator, Verdünnungssystem und Expositionssystem an Zellkulturen (A549 und THP-1) gegenüber den entstehenden Stäuben bestimmt (Zytotoxizität).

 

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26. May. 2018

Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients

DOI: 10.1080/17435390.2018.1472310

Beyeler S1,2, Chortarea S3,4, Rothen-Rutishauser B3, Petri-Fink A3, Wick P4, Tschanz SA5, von Garnier C1,2, Blank F1,2.

1 Department of BioMedical Research , University of Bern , Bern , Switzerland.
2 Department of Pulmonary Medicine , University Hospital of Bern , Bern , Switzerland.
3 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg , Switzerland.
4 Laboratory for Particles-Biology Interactions, Empa Swiss Federal Laboratories for Material Science and Technology , St. Gallen , Switzerland.
5 Institute of Anatomy, University of Bern , Bern, Switzerland.

This paper shows the investigation of acute effects of occupational multi-walled carbon nanotubes exposure in primary epithelial lung cell cultures from potentially susceptible individuals with COPD.

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10. May. 2018

Combining MucilAir™ and Vitrocell® Powder Chamber for the In Vitro Evaluation of Nasal Ointments in the Context of Aerosolized Pollen

https://doi.org/10.3390/pharmaceutics10020056 

Julia Metz 1,2, Katharina Knoth 1, Henrik Groß 1, Claus-Michael Lehr 1,2,3, Carolin Stäbler 4, Udo Bock 5, and Marius Hittinger 1
1Department of Drug Delivery, PharmBioTec GmbH, 66123 Saarbrücken, Germany
2Department of Biopharmaceutics and Pharmaceutical Technology, Department of Pharmacy, Saarland University, 66123 Saarbrücken, Germany
3Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Center for Infection Research (HZI), 66123 Saarbrücken, Germany
4Department of Scientific Affairs Consumer Health, Bayer Vital GmbH, 51368 Leverkusen, Germany
5Bock Project Management, 54456 Tawern, Germany

In this study two innovative in vitro techniques were combined: The Vitrocell® Powder Chamber and the human cell based MucilAir™ system. Their combined use allows the investigation of the effect of aerosolized pollen and appropriate drugs on a human mucosal tissue, a similar scenario finding in humans. The release of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) was quantified as an initial cellular response to pollen contact and to confirm the protective effect of drugs.

 

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12. Apr. 2018

Development, qualification, validation and application of the Ames test using a VITROCELL® VC10® smoke exposure system

https://doi.org/10.1016/j.toxrep.2018.04.003

Kathy Fowlera, Wanda Fieldsa, Victoria Hargreavesb, Lesley Reeveb, Betsy Bombicka,
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA2
b Covance Laboratories Ltd., North Yorkshire, UK

The article shows the successfull qualification of the Vitrocell VC10 smoking robot. The robot was used to  expose bacterial cells to cigarette whole smoke generated from 3R4F reference cigarettes and Eclipse cigarettes. A comparison was made between whole smoke from a tobacco heating and a combustible cigarette.

 

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5. Apr. 2018

A Case Study for the Comparison of In Vitro Data Across Multiple Aerosol Exposure Studies with Extrapolation to Human Dose

DOI: 10.1089/aivt.2017.0042

David Thorne, Emma Bishop, Linsey Haswell, and Marianna Gaca
British American Tobacco Group R&D, Southampton, United Kingdom

This case study has demonstrated that dosimetry techniques using particulate markers can allow in vitro biological data generated on five independent aerosol exposure system studies, under contrasting experimental designs and with different cell types, to be directly compared.

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28. Mar. 2018

Characterization of a Vitrocell VC1 Using Nicotine Dosimetry: An Essential Component Toward Standardized In Vitro Aerosol Exposure of Tobacco and Next Generation Nicotine Delivery Products

DOI: 10.1089/aivt.2018.0001
Holger Behrsing,1 Mario Aragon,1 Jason Adamson,2 Devin Sheehan,1 Marianna Gaca,2 Rodger Curren,1 and Erin Hill1
1Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
2R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

 

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11. Mar. 2018

A setup to test toxicity of alcohol inhalation from consumer products

User Group Meeting 2018

Dr. Monique Manche1, Dr. Fabrice Nesslany2, Tobias Krebs3
1Laboratoires Anios, 59260 Lille-Hellemmes, France
2Laboratoire de toxicologie génétique, Institut Pasteur de Lille, 59000 Lille, France
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

Genotoxicity tests (in vitro micronucleus test and comet assay) have been conducted on p53 competent pulmonary cells, with alcohol administration as vapours to obtain data under conditions more representative of actual exposure conditions via the pulmonary route.

 

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11. Mar. 2018

Update on FTIR integration

User Group Meeting 2018

Dr. Martin Kieninger1, Tobias Krebs2

1ANSYCO GmbH, Gasmet Group, 76131 Karlsruhe, Germany

2VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

The poster shows a reliable, easy to use online analysis tool with fast response and a wide range of components due to Calcmet library. Ideal for quality control and dose monitoring.


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11. Mar. 2018

Photometer Calibration Method

User Group Meeting 2018

Johannes Sood

VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The poster shows a reliable method for the photometer calibration.

 

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