Ali Doryab 1,2, Mehmet Berat Taskin 3, Philipp Stahlhut 3, Andreas Schröppel 1,2, Sezer Orak 1,2, Carola Voss 1,2, Arti Ahluwalia 4,5, Markus Rehberg 1,2, Anne Hilgendorff 1,2,6, Tobias Stöger 1,2, Jürgen Groll 3 and Otmar Schmid 1,2
1 Comprehensive Pneumology Center Munich, Member of the German Center for Lung Research, Munich, Germany,
2 Helmholtz Zentrum München—German Research Center for Environmental Health, Institute of Lung Biology and Disease, Munich, Germany,
3 Department of Functional Materials in Medicine and Dentistry, Bavarian Polymer Institute, University of Würzburg, Würzburg, Germany,
4 Research Center “E. Piaggio”, University of Pisa, Pisa, Italy,
5 Department of Information Engineering, University of Pisa, Pisa, Italy,
6 Center for Comprehensive Developmental Care (CDeCLMU), Dr. von Haunersches Children’s Hospital University, Hospital of the Ludwig-Maximilians University, Munich, Germany
We have recently introduced a novel porous and elastic membrane for in vitro cell-stretch models of the lung cultured under ALI conditions (Doryab et al., 2020). This innovative hybrid biphasic membrane, henceforth referred to as Biphasic Elastic Thin for Air-liquid culture conditions (BETA) membrane, was developed to optimize membrane characteristics for the two phases of cell-stretch experiments under ALI conditions, namely the initial cell seeding, attachment and growth phase under submerged cell culture conditions (phase I) followed by an ALI acclimatization and cell-stretch phase at the ALI (phase II). This patented aerosol-cell exposure unit has recently been made commercially available as VITROCELL® Cloud MAX.