Exposure at the air/liquid interface

The exposure of mammalian cells or tissues to airborne substances is frequently performed under submerged conditions. In doing so, the test substances are dosed into the culture medium. This procedure results in an undesired interaction of the formerly airborne substances with the medium, causing limitations for authentic analysis.

VITROCELL® Flow Illustration

In response to the scientific need to expose in physiologically relevant conditions, VITROCELL® systems have been specifically designed and engineered to enable direct exposure of mammalian cells or tissue at the air/liquid interface where the cell systems are not covered with culture medium. Researchers can thus use all cell types cultivated on microporous membranes. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.

Submerged Cultivation and Exposure in Incubator

A Medium above cells
B Cells on membrane
C Medium below cells

Interaction of test components with culture medium
Low sensitivity

Submerged Cultivation and Exposure in Incubator

A Cells in medium

Interaction of test components with culture medium
Low sensitivity

Air/Liquid Cultivation and
Exposure in Exposure Module

A Direct and controlled exposure of test atmosphere to cells
B Cells on membrane
C Medium below cells

No losses
No reaction of principle components with culture medium
High sensitivity of system

Exposure Principles

Learn more about the Exposure Principles for the Air/Liquid Interface

Continuous Flow Exposure Systems

Single Droplet Sedimentation Systems

Dry Powder Exposure Systems

Interested in

Exposure at the air/liquid interface

We are here to help.

Don't hesitate to contact us on your questions regarding our products and services!

chevron-down