Advanced in vitro exposure systems.

27. Oct. 2022

VITROCELL® sQCM 12: Data Interpretation

The Quartz Crystal Microbalance is a powerful tool for dosimetry in VITROCELL Exposure Systems. However, interpreting live-data from a sQCM can be misleading, as there is a lot going on in parallel during measurements.

VITROCELL Application Note

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26. Sep. 2022

Product News 06-9/2022

VITROCELL® Cloud Alpha MOVE

For fully automated and repeated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts. Testing of up to 12 dose ranges in one experiment.

The VITROCELL® Cloud Alpha MOVE is our newest innovation and presents another great leap forward in fully automated exposure of cell cultures. It is the ideal device to obtain a dose-response relationship in one experiment.

VITROCELL® Cloud Alpha MOVE  (PDF)

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1. Jun. 2022

Product News 05-4/2022

VITROCELL® PowderX

For cell culture exposure to smallest quantities of dry powders

The VITROCELL® PowderX allows for aerosolisation of small quantities of dry powders and particle deposition on cells cultured on 12- or 24-well sized inserts. The system features biological barrier integration and continuous sampling from the basolateral compartment.

VITROCELL® PowderX  (PDF)

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9. Dec. 2021

Iota-carrageenan extracted from red algae is a potent inhibitor of SARS-CoV-2 infection in reconstituted human airway epithelia

https://doi.org/10.1016/j.bbrep.2021.101187

David Bovard b, Marco van der Toorn b, Walter K. Schlage a, Samuel Constant c, Kasper Renggli b, Manuel C. Peitsch b, Julia Hoeng b,
a Biology Consultant, Max-Baermann-Str. 21, 51429, Bergisch Gladbach, Germany
b PMI R&D, Philip Morris Products S.A, Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland
c Epithelix Sarl, 18 Chemin des Aulx, Plan-les-Ouates, 1228, Geneva, Switzerland

 

This study successfully demonstrates the exposure of human bronchial epithelial cultures to defined doses of nebulized Iota-carrageenan which were reproducibly generated and administered using the VITROCELL Cloud 12 system. 
The aim of this study was to further investigate the efficacy and safety of IC treatment on SARS-CoV-2 infection by using advanced in vitro models of human respiratory epithelium, the primary target and entry port of SARS-CoV-2. The experimental models were 3D cultures of reconstituted bronchial and nasal epithelia, representing the surface of the human upper respiratory tract. This apical exposure of reconstructed epithelia more closely mimics real-life exposure conditions and the absence of toxicity or any functional or structural impairment of the bronchial mucociliary epithelium demonstrates that topical treatment with nebulized IC is well tolerated at the effective concentrations. 

 

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3. Nov. 2022

Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module

https://doi.org/10.1016/j.toxrep.2022.11.001

Brian M. Keyser1, Robert Leverette1, Michael Hollings2, Adam Seymour2, Randy A. Weidman3, Carlton J. Bequette3, Kristen Jordan1
1 RAI Services Company; Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
2 Labcorp Early Development Laboratories Ltd., Harrogate, North Yorkshire, UK
3 RJ Reynolds Tobacco Company; 950 Reynolds Blvd., Winston-Salem, NC 27106, USA

 

Highlights

  • Characterization of two 48 well exposure modules with three different product types
  • Comparison of these exposure modules using four different dosimetry techniques
  • Ability to differentiate the aerosol deliveries between different dilution airflows

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28. Oct. 2022

ESTIV 2022 - International Congress of the European Society of Toxicology In Vitro

November 21–25, 2022, Barcelona (ES)

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Categories: Exhibitions

Tags: congress

9. Sep. 2022

ICT 2022 - International Congress of Toxicology

September 18–21, 2022, Maastricht (NL)

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Categories: Exhibitions

Tags: congress

22. Aug. 2022

Carbon nanoparticles adversely affect CFTR expression and toxicologically relevant pathways

https://doi.org/10.1038/s41598-022-18098-8

Torben Stermann1, Thach Nguyen1, Burkhard Stahlmecke2, Ana Maria Todea2, Selina Woeste1, Inken Hacheney1, Jean Krutmann1,3, Klaus Unfried1, Roel P. F. Schins1 & Andrea Rossi1
1IUF – Leibniz-Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 Duesseldorf, Germany. 
2IUTA – Institut für Energie- und Umwelttechnik e.V., Duisburg, Germany. 
3Medical Faculty, Heinrich Heine University, Düsseldorf, Germany. *email: andrea.rossi@iuf-duesseldorf.de

 

In the present study the 16HBE14o- cells were exposed in an automated exposure station at air-liquid interface conditions, using the controlled generation of carbon nanoparticles (CNP) aerosols by spark-ablation. This study reports that CNP exposure leads to decrease cystic fibrosis transmembrane conductance regulator (CFTR) expression accompanied by transcriptomic signs of oxidative stress, apoptosis and DNA damage.

 

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21. Jun. 2022

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

https://doi.org/10.1016/j.envint.2022.107366

Michal Pardoa, Svenja Offerbc, Elena Hartnerbc, Sebastiano Di Bucchianicob, Christoph Bisigb, Stefanie Bauerb, Jana Pantzkebc, Elias J. Zimmermannbc, XinCaobc, Stephanie Binderbc, Evelyn Kuhnb, Anja Huberb, Seongho Jeongbc, Uwe Käferbc, Eric Schneiderc,[...]

a Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, ISR-7610001 Rehovot, Israel
b Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics (CMA), Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
c Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr.-Lorenz-Weg 2, D-18059 Rostock, Germany
d Department of Environmental and Biological Sciences, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
e University of the Bundeswehr Munich, Institute for Chemistry and Environmental Engineering, Werner- Heisenberg-Weg 39, D-85577 Neubiberg, Germany
f Institute for Environmental Studies, Faculty of Science, Charles University, Albertov 6, CZE-12800 Prague, Czech Republic
g Department of Applied Physics, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
h Institute of Energy and Climate Research, Troposphere (IEK-8), Forschungszentrum Jülich GmbH, Wilhelm-Johen-Str., D-52428 Jülich, Germany
i Department of Environmental Sciences, University of Basel, Klingelbergstr. 27, CH-4056 Basel, Switzerland
j Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Str. 10, D-13125 Berlin, Germany
k Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
l The Mantoux Bioinformatics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot 76100, Israel

 

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15. Jun. 2022

Characterization of Aerosol Deliveries from Combustible Cigarettes, Heated Tobacco Products, and Electronic Nicotine Delivery Systems Using the Vitrocell Ames 48

DOI: 10.1089/aivt.2022.0001

Brian M. Keyser,1 Robert Leverette,1 Michael Hollings,2 Adam Seymour,2 Randy J. Weidman,3 Carlton J. Bequette,3 and Kristen Jordan1
1 Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, North Carolina, USA.
2 Labcorp Early Development Laboratories Ltd., Harrogate, North Yorkshire, United Kingdom.
3 RJ Reynolds Tobacco Company, Winston-Salem, North Carolina, USA

 

This study utilized a Vitrocell Ames 48 exposure module climatic chamber. Cigarette smoke and eHTP/ENDS aerosol was generated using a Vitrocell VC10. Different concentrations of whole smoke or aerosol were achieved by altering the diluting airflow using mass flow controllers  The dosimetry measure used for assessing both combustible and NGTP aerosols in this characterization exercise was the laser photometer purchased from Vitrocell Systems and harmonized for each tobacco product type used in this study. To evaluate the multiple dosimetry techniques, pairwise comparisons were made between the two Vitrocell Ames 48 exposure modules for each of the three test articles. 

 

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