Aerosol exposure for breathing AXLung-on-Chip in close cooperation with AlveoliX
The VITROCELL® Cloud Alpha AX12 is our innovation in the Cloud family and presents a great leap forward in automated exposure of cell cultures with breathing function. It combines highly efficient testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12-, 24- and 96-well). Its functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the Air/Liquid Interface have never been easier.
It is suitable for the nebulization of solutions and suspensions. Fields of application are, but are not limited to, screening of inhaled drugs and toxicity testing of inhaled substances such as chemicals, nanoparticles and airborne pathogens.
A: Direct and controlled exposure of test atmosphere to cells
B: Cells on ultrathin AX membrane
C: Media below cells
1: Inlet well
2: Cell well
3: Outlet well
4: Fluidic chamber
The AXLung-on-Chip System allows in vitro modeling in outmost physiological conditions by replicating the essential key features of the lung microenvironment.
The combination of the breathing-induced cyclic stretch and the optimized ultrathin cell culture substrate (AX membrane) preserves lung cells’ functionality and phenotype. The technology provides answers to fundamental questions about lung biomechanics and cell biology. Furthermore, it is a powerful tool to recreate healthy, diseased, and personalized in vitro models, which can be implemented in drug development to test molecule efficacy and safety, and in toxicity assessment of e. g. pollutants, chemicals and consumer goods. This will hopefully lead to more animal free product development in the future.
Aside the AX12, the system is made up of three further components: two pneumatic controllers, AX Breather and AX Exchanger, and the AX Dock which is the interface between these two devices and the AX12.
A nature-inspired concept is used to recreate the breathing motion. The ultrathin AXmembrane (blue) is deflected by applying negative pressure inside the basal chip chamber through an integrated micro-diaphragm (grey).
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