Poster, SOT 64th Annual Meeting and ToxExpo, March 16–20, 2025, Orlando, Florida.
Preliminary test of toxicological responce of brake wear particles using Vitrocell Cloud Alpha 12 system under airliquid interface (ALI) conditions, mimicking more realistic exposure conditions to airborne PM2.5.
- In the co-culture the formation of a functional alveolar-capillary barrier is assessed using IF staining of epithelial cells of Type II (Hopx) and ciliated cells (acetylated tubulin).
- A homogeneous, tight cell layer is observed, with a thickness ranging from 10 to 15 µm for A549 cells.
- Nebulization of brake wear particles is performed using the Vitrocell Cloud Alpha 12 system, resulting in a uniform distribution of particles across test inserts.
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