Development of the in vitro micronucleus test for exposure to whole aerosol from cigarettes and heated tobacco products

Poster, SOT 64th Annual Meeting and ToxExpo, March 16–20, 2025, Orlando, Florida.

I. Crooks¹, D. Thorne¹, R. Leverette², M. Hollings³, D. Breheny^1
1B.A.T. (Investments Limited, Southampton, United Kingdom
²RAI Services Company, Winston-Salem, NC 27106, USA
³Labcorp Early Development Laboratories, Harrogate, United Kingdom

Introduction
In the genotoxic assessment of tobacco products, traditional approaches involve capturing the two phases of the aerosol, such as pad-collected total particulate matter (TPM) and/or gas-vapour phase (GVP) in solvents and applied to cell cultures. This Approach may under-represent the interactions of the whole aerosol (WA). The exposure of cells to WA at the air-liquid interface (ALI) mitigates the need for this fractionation. The aim of this study was to investigate the potential of WA from a reference cigarette (1R6F) and a Heated Tobacco Product (HTP) to induce micronuclei in cells exposed at the ALI.

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