Aerosol exposure for breathing AXOrgan-on-Chip in close cooperation with AlveoliX
The VITROCELL® Cloud Alpha AX12 is part of our Cloud family and presents a great leap forward in automated exposure of cell cultures with breathing function. It combines highly efficient testing with ease of use. The development is based on the well-known and widely published VITROCELL® Cloud formats (6-, 12-, 24-, and 96-well). It features an all-in-one control unit that allows for fully automated processes.
Whether for drug discovery and preclinical studies, substances of toxicological concern, environmental pollutants, chemicals, viruses or other biological samples: AlveoliX’ and VITROCELL’s platform can replicate diverse exposure scenarios, enabling most comprehensive in vitro research.
The VITROCELL® Cloud Alpha AX12 is designed for small nebulization volumes. The recommended nebulization volume is 300 μl. Therefore, the device is particularly suitable for testing materials even when quantities are limited.
A: Direct and controlled exposure of test atmosphere to cells
B: Cells on ultrathin AXmembrane
C: Media below cells
1: Inlet well
2: Cell well
3: Outlet well
4: Fluidic chamber
The AXBarrier-on-Chip System allows in vitro modeling in outmost physiological conditions by replicating the essential key features of all organs in vitro, including lung, skin and eyes.
The technology is a powerful tool to recreate healthy, diseased and personalized in vitro models. It provides answers to fundamental questions about biomechanics and cell biology. Furthermore, the method can be implemented in drug development to test molecule efficacy and safety, and in toxicity assessment of e. g. pollutants, chemicals and consumer goods. This will hopefully lead to more animal free product development in the future.
A nature-inspired concept is used to recreate the breathing motion. The ultrathin AXmembrane is deflected by applying negative pressure inside the basal chip chamber through an integrated micro-diaphragm. The combination of the breathing-induced cyclic stretch and the optimized ultrathin cell culture substrate (AXmembrane) preserves lung cells’ functionality and phenotype.
The system offers a selection of three types of vibrating mesh nebulizers, with droplet MMAD ranges of 4.0 – 6.0 µm, 4.0 – 6.0 µm, 2.5 – 6.0 µm, and 4.0–6.0 µm, 12 µm, 17 µm, and 20 µm.
With a recommended nebulization volume of 300 µL, the Cloud Alpha AX12 is especially well-suited for experiments where only small amounts of test material are available.
A nature-inspired concept is used to recreate the breathing motion. The ultrathin AXmembrane (blue) is deflected by applying negative pressure inside the basal chip chamber through an integrated micro-diaphragm (grey).
The QCM6 sensor seamlessly integrates into the Cloud Alpha AX12 and Powder AX12 exposure module. It offers precision measurement of deposited mass, down to nanograms/cm2. All results are logged within the VITROCELL® Monitor software, where they are presented graphically and can be effortlessly exported as .csv files for for further analysis.
VITROCELL® Cloud Alpha Single Droplet Sedimentation Systems are user-friendly. The aerosol is applied for a short duration of approximately 7–10 minutes and can be repeated several times to obtain a dose-response profile. All components are easy to clean.
Start dynamic experiments in mono- or coculture with the AX Barrier-on-Chip System to create advanced in vitro models. For a smooth integration into your laboratory, the membrane comes with a proprietary ECM coating. This allows the users to seed directly the cells inside the AX12 to create their model of interest.
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