For exposure of 12- and 24-well sized cell culture inserts in three separated compartments: Testing of up to
three dose ranges or three different substances in one experiment.
The VITROCELL® Cloud Alpha TRIO presents another innovative leap forward in the exposure of cell cultures. This versatile device enables the acquisition of three dose relationships in a single experiment or facilitates working with three different substances simultaneously. The latter procedure is particularly interesting for users which need to test and compare larger numbers of different substances. The development is based on the well-known and frequently published VITROCELL® Cloud formats.
The new Cloud Alpha TRIO is capable to expose mammalian cell cultures in 12-and 24-well sized inserts. The Cloud system is suitable for nebulization of solutions and suspensions. Fields of application are screening of inhalable drugs, assessing the toxicity of inhaled substances like chemicals or nanoparticles, and conducting research on viruses.
It comes with a choice of three types of vibrating mesh nebulizers having droplet MMAD ranges of 2.5–6.0 µm, 2.5–4.0 µm, 4.0–6.0 µm, and in an advanced version with 9.0–12.0 µm. The device is particularly suitable for testing whenever limited quantitities of testing materials are available.
In response to the scientific need to expose in physiologically relevant conditions, the VITROCELL® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired interaction of the formerly airborne substances with the culture media.
Cell systems cultivated on membrane inserts are exposed at the Air/Liquid Interface (ALI) so that the test substances are received directly. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
A: Direct and controlled exposure of test atmosphere to cells
B: Cells on membrane
C: Media below cells
Exposure of 12-well sized culture inserts in three rows at four replicates.
The Quartz Crystal Microbalance sensor is integrated in the Cloud Alpha MOVE exposure module. It is capable of measuring the deposited mass in nanogram/cm2. Results are recorded in the VITROCELL® Monitor software. Data is presented in graphs and stored in MS Excel®.
All commercial brands are supported.