For fully automated and repeated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts.
Testing of up to 12 dose ranges in one experiment.
The VITROCELL® Cloud Alpha MOVE presents another great leap forward in fully automated exposure of cell cultures. It is the ideal device to obtain a dose-response relationship in one experiment. The development is based on the well-known and frequently published VITROCELL® Cloud formats. It’s functionality enables a fully automated process with an all-in-one control unit.
The Cloud Alpha MOVE is capable to expose mammalian cell cultures in Corning® HTS Transwell® 96-Well Permeable Support Systems as well as 12-and 24-well sized inserts. The Cloud system is suitable for nebulization of solutions and suspensions. Fields of application are screening of inhaled drugs, toxicity testing of inhaled substances such as chemicals or nanoparticles and virus research.
It comes with a choice of 3 types of vibrating mesh nebulizers having droplet MMAD ranges of 2.5–6.0 µm, 2.5–4.0 µm, 4.0–6.0 µm and in a special version with 9.0–12.0 µm. The device is particularly suitable for testing whenever small quantitities of testing materials are available.
All VITROCELL® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface in physiologically relevant conditions. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired interaction of the formerly airborne substances with the culture media.
This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
A: Direct and controlled exposure of test atmosphere to cells
B: Cells on membrane
C: Media below cells
VITROCELL® exposure systems have been specifically designed and engineered to give researchers the possibility of directly exposing mammalian cells or tissue at the air/liquid interface. Thus, all cell types cultivated on microporous membranes can be used. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
The Quartz Crystal Microbalance sensor is integrated in the Cloud Alpha MOVE exposure module. It is capable of measuring the deposited mass in nanogram/cm2. Results are recorded in the VITROCELL® Monitor software. Data is presented in graphs and stored in MS Excel®.