For 12 cell culture inserts (12-well size)
The VITROCELL® 12/12 PLUS has been specifically designed and engineered for advanced direct exposure studies of human cell cultures at the air/liquid interface. It enables controlled exposure to airborne substances such as gases, complex mixtures, nanoparticles and fibers, while authentically simulating physiologically relevant human exposure conditions.
Built on a robust, compact module concept, the system features an electropolished stainless-steel casing and is fully autoclavable at 121 °C / 250 °F for 20 minutes. The base configuration provides 12 compartments in a 4 x 3 layout, allowing the simultaneous exposure of 3 dilutions plus 1 clean-air control, each with 3 replicates. This makes the VITROCELL® 12/12 PLUS an efficient solution for reproducible dose-response studies in a compact format.
For maximum flexibility, the system supports 12-well and 24-well sized cell culture inserts using dedicated adapter sets, while electronic temperature control ensures constant and reliable unit temperature throughout the exposure process.
The PLUS configuration extends the system with an additional row of 4 media compartments, increasing the total capacity to 16 positions. These additional positions can be used either for further cell culture inserts or for integrated dosimetry. Depending on the application, the dosimetry positions can be equipped with Quartz Crystal Microbalances, TEM Grid Holders or stainless-steel Dosimetry Inserts for aerosol trapping in liquids. This makes the VITROCELL® 12/12 PLUS a highly versatile platform for combining cell exposure and dosimetric assessment in one integrated system.
For equal temperature control of all tubings and aerosol ducts. The professional solution to avoid condensation during the exposure to liquid aerosols.
The aerosol is guided from the central duct to the modules via isokinetic sampling probes enabling high reproducibility and uniformity of results.
The inlets aspirate directly from the constant flow of test substance.The isokinetic distribution system is made of stainless steel.
The optional dilution systems are equipped with air jets for dynamic dilution to obtain different dose / response results per row @ 3 replicates.
A complete rack system for fixation of components features a docking station with sliding base for fast and safe connections. The unique quick-connect system allows for tubeless connections of distribution and distribution systems.
Special sealing and connection mechanisms guarantee a hermetic connection of the base module with the aerosol exposure top.
The aerosol inlet-stream flows through specifically shaped inlets. They are available in stainless steel or stainless steel with unique VITROGLIDE surface treatment for lowest adhesion results. The stainless steel / VITROGLIDE inlets are specifically designed for aerosols containing nanoparticles.
The aerosol inlets are connected to the distribution or dilution systems. Extraction from the module takes place via small holes using a controlled vacuum flow. VITROCELL® calibration valves, which are connected to a vacuum pump control flow rates effectively. The aerosol exposure top features an in/outlet for heating the water circuit, ensuring corresponding temperatures with the base module. It is made of high-quality anodized aluminum.
VITROCELL® exposure systems have been specifically designed and engineered to give researchers the possibility of directly exposing mammalian cells or tissue at the air/liquid interface. Thus, all cell types cultivated on microporous membranes can be used. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
The QCM sensor can be integrated in the exposure module. It is capable of measuring the deposited mass in nanogram/cm2 per second. Results are reported online by the VITROCELL® Monitor software. Data is presented in graphs and stored in MS Excel®.
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