For 12 cell culture inserts (12-well size)
The VITROCELL® 12/12 module system has been specifically designed and engineered to facilitate the research of human cell cultures direct exposure to airborne substances such as gases, complex mixtures, nanoparticles and fibers. The system authentically simulates the conditions of human physiological exposure.
The VITROCELL® 12/12 modules’ casing is made of electropolished stainless steel. It is designed with 12 (4x3) compartments for 12-well or 24-well sized cell culture inserts and is fully autoclavable at 121° C (250° F) for 20 min. The capability to expose 3 dilutions plus 1 clean-air control with 3 replicates in a compact module is its’ outstanding feature. Through the employment of a special adapter set, this module can also be flexibly combined for use with 24-well cell culture inserts. A constant unit temperature is guaranteed using an electronic temperature control.
Special sealing and connection mechanisms guarantee a hermetic connection of the base module with the aerosol exposure top.
The aerosol inlet-stream flows through specifically shaped inlets. They are available in stainless steel or stainless steel with unique VITROGLIDE surface treatment for lowest adhesion results. The stainless steel / VITROGLIDE inlets are specifically designed for aerosols containing nanoparticles.
The aerosol inlets are connected to the distribution or dilution systems. Extraction from the module takes place via small holes using a controlled vacuum flow. VITROCELL® calibration valves, which are connected to a vacuum pump control flow rates effectively. The aerosol exposure top features an in/outlet for heating the water circuit, ensuring corresponding temperatures with the base module. It is made of high-quality anodized aluminum.
VITROCELL® exposure systems have been specifically designed and engineered to give researchers the possibility of directly exposing mammalian cells or tissue at the air/liquid interface. Thus, all cell types cultivated on microporous membranes can be used. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.
For equal temperature control of all tubings and aerosol ducts. The professional solution to avoid condensation during the exposure to liquid aerosols.
Universal module with optional adaptor sets for:
All commercial brands are supported.
A complete rack system for fixation of components features a docking station with sliding base for fast and safe connections. The unique quick-connect system allows for tubeless connections of distribution and distribution systems.
The aerosol is guided from the central duct to the modules via isokinetic sampling probes enabling high reproducibility and uniformity of results.
The inlets aspirate directly from the constant flow of test substance.The isokinetic distribution system is made of stainless steel.
The optional dilution systems are equipped with air jets for dynamic dilution to obtain different dose / response results per row @ 3 replicates.
The VC 12/12 PLUS System has an additional row with 4 media compartments - increasing the total amount to 16 positions. The 4 additional positions may be used for cell culture inserts or for dosimetry. The dosimetry positions can be equipped with either Quartz Crystal Microbalances, TEM Grid Holders or stainless Dosimetry Inserts for aerosol trapping in liquids.
The QCM sensor can be integrated in the exposure module. It is capable of measuring the deposited mass in nanogram/cm2 per second. Results are reported online by the VITROCELL® Monitor software. Data is presented in graphs and stored in MS Excel®.
The sQCM 12 microbalance can be mounted in each row. Alternatively stainless steel Dosimetry Inserts or TEM Grid Holders are fitted here.