The Organ-on-Chip Exposure Solution for Dry Powders
The VITROCELL® Powder AX12 Exposure System is specificalley designed for direct aerosolization of smallest quantities of dry powders without the need of transfering them in suspension first. Thus, especially scarce and very expensive materials can be well used with this method, such as new drug candidates or particle samples from the environment.
Here quantities from 1 – 100 mg can be aerosolized. Aerosolization is performed directy into the cell culture exposure chamber. Aerosolization can be repeated a few times to obtain a dose-response profile.
Powder AX 12 allows for aerosolisation of small quantities of dry powders (1-100 mg) on the AX12 plate.
Powder sample is placed in quick-lock loading system.
Powder sample is aerosolized under high pressure to break up agglomerates for homogeneous dispersion in the expansion chamber.
Powder sample settles on the AX12 plate.
A: Direct and controlled exposure of test atmosphere to cells
B: Cells on ultrathin AXmembrane
C: Media below cells
1: Inlet well
2: Cell well
3: Outlet well
4: Fluidic chamber
The AXLung-on-Chip System allows in vitro modeling in outmost physiological conditions by replicating the essential key features of the lung microenvironment.
The combination of the breathing-induced cyclic stretch and the optimized ultrathin cell culture substrate (AX membrane) preserves lung cells’ functionality and phenotype. The technology provides answers to fundamental questions about lung biomechanics and cell biology. Furthermore, it is a powerful tool to recreate healthy, diseased, and personalized in vitro models, which can be implemented in drug development to test molecule efficacy and safety, and in toxicity assessment of e. g. pollutants, chemicals and consumer goods. This will hopefully lead to more animal free product development in the future.
Aside the AX12, the system is made up of three further components: two pneumatic controllers, AX Breather and AX Exchanger, and the AX Dock which is the interface between these two devices and the AX12.
A nature-inspired concept is used to recreate the breathing motion. The ultrathin AXmembrane (blue) is deflected by applying negative pressure inside the basal chip chamber through an integrated micro-diaphragm (grey).
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