Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward.

January 15, 2020

StefanPfuhlera, Janvan Benthemb, RodgerCurrenc, Shareen H.Doakd, MariaDusinskae, MakotoHayashif, Robert H.Heflichg, DarrenKiddh, DavidKirklandi, YangLuanj, GladysOuedraogok, KerstinReisingerl, ToshioSofunim, Frédériquevan Ackern, YingYango, RaffaellaCorvip
a Procter and Gamble, Mason Business Centre, Mason, OH, USA
b National Institute for Public Health and the Environment, Centre for Health Protection, Bilthoven, the Netherlands
c Institute for In Vitro Sciences, Inc., Gaithersburg, MD, USA
d Swansea University Medical School, Singleton Park, Swansea, SA2 8PP, Wales, UK
e Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute for Air Research, Kjeller, Norway
f makoto international consulting, Ebina, Japan
g U.S. Food and Drug Administration/National Center for Toxicological Research, Jefferson, AR, USA
h Covance Laboratories Ltd, Otley Road, Harrogate, HG3 1PY, UK
i Kirkland Consulting, PO Box 79, Tadcaster, LS24 0AS, UK
j School of Public Health, Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, PR China
k L’Oréal R&I, Aulnay-sous-bois, France
l Henkel AG & Co KGaA, Duesseldorf, Germany
m Formerly National Institute of Health Sciences, Tokyo, Japan
n Triskelion B.V., Zeist, the Netherlands
o Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, PR China
p European Commission, Joint Research Centre (JRC), Ispra, Italy


• Extensive progress made in development of 3D organ-based genotoxicity assays.
• 3D culture models represent major exposure routes: dermal, oral, inhalation.
• The 3D skin comet and MN assays are considered mature and sufficiently validated.
• Liver and airway model-based genotoxicity assays show promise but are at early stage.&lt

Use of three-dimensional (3D) tissue equivalents in toxicology has been increasing over the last decade as novel preclinical test systems and as alternatives to animal testing. In the area of genetic toxicology, progress has been made with establishing robust protocols for skin, airway (lung) and liver tissue equivalents. In light of these advancements, a “Use of 3D Tissues in Genotoxicity Testing” working group (WG) met at the 7th IWGT meeting in Tokyo in November 2017 to discuss progress with these models and how they may fit into a genotoxicity testing strategy. The workshop demonstrated that skin models have reached an advanced state of validation following over 10 years of development, while liver and airway model-based genotoxicity assays show promise but are at an early stage of development. Further effort in liver and airway model-based assays is needed to address the lack of coverage of the three main endpoints of genotoxicity (mutagenicity, clastogenicity and aneugenicity), and information on metabolic competence. The IWGT WG believes that the 3D skin comet and micronucleus assays are now sufficiently validated to undergo an independent peer review of the validation study, followed by development of individual OECD Test Guidelines.

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