The INSPIRE Initiative: Using human cell-based in vitro systems to assess respiratory toxicity of surfactants delivered as liquid or aerosols

March 12, 2024

Poster, SOT 63rd Annual Meeting and ToxExpo, March 10–14, 2024, Salt Lake City, Utah.

Monita Sharma1, Andreas O. Stucki1, Sandra Verstraelen2, An Jacobs2, Nuria Roldan1, Karen Hollanders2, Jo Van Laer2, Sylvie Remy2, Evelien Frijns2, Amy J. Clippinger1
1PETA Science Consortium International e.V., Stuttgart, DE;
2Flemish Institute for Technological Research (VITO), Health Department, Mol, BE

The work presented here builds upon recently published data for the testing of silane vapors (Sharma et al. 2023) and focuses on surfactant testing. Surfactants are of interest to regulatory agencies because of their use in various products (e.g., soaps, adhesives, and herbicides) as emulsifiers, wetting agents, detergents, foaming agents, or dispersants. Surfactants can interact with the amphiphilic cell membranes compromising membrane integrity and causing general cytotoxicity. When inhaled, surfactants can also perturb the resident surface lining fluid in the lower respiratory tracts that may lead to a collapse of the alveoli (atelectasis). In this study, a human bronchial epithelial cell line (BEAS-2B) and a reconstructed human tissue model (MucilAir ) were used to assess the toxicity of two surfactants ⎯Triton X-100 (non-ionic surfactant; CAS Number: 9002-93-1) and oleoyl sarcosine (anionic surfactant; CAS Number: 110-25- 8)⎯exposed to cells as aerosols or liquids (pipetting).

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