Advanced in vitro exposure systems.

30. Sep. 2013

Comparison of epithelial cells inflammatory response from the human respiratory tract after formaldehyde exposure

Poster EUROTOX 2013. Interlaken, Switzerland.

September 2013

Authors
Gaëlle Bardet1,2, Charles Persoz1, Thomas Loret1, Isabelle Momas1, Sophie Achard1, Nathalie Seta1

1 Université Paris Descartes, Faculté de Pharmacie, EA4064 Impact sanitaire des pollutions, 75006 Paris, France
2 Agence de l’environnement et de la Maîtrise de l’Energie 20, avenue du Grésillé, BP 90406, 49004 Angers Cedex 01 France
Correspondence: gaelle.bardet@etu.parisdescartes.fr

 

The Exposition of Formaldehyde as a major indoor air pollutant on epithelial cells from different parts of the human respiratory tract is demonstrated in the results if IL-8 and MCP assay.

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29. Jul. 2013

Evaluation method for the cytotoxicity of cigarette smoke byin vitro whole smoke exposure

https://doi.org/10.1016/j.etp.2013.07.004

Xiang Li, Cong Nie, Pingping Shang, Fuwei Xie, Huimin Liu, Jianping Xie

Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China

With the development of direct exposure technology at the air/liquid interface, the toxicological responses of fresh WS can be evaluated. The whole smoke of the 3R4F reference cigarette and two experimental cigarettes were tested on Chinese hamster ovary (CHO) cells to evaluate the endpoints Ames Salmonella reversemutagenicity assay and in vitro micronucleus assay for genotoxicity and neutral red uptake (NRU)assay for cytotoxicity.

 

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31. Mar. 2013

New on SOT San Antonio

VITROCELL® 12/4 Module

The new exposure module for inserts in the 12-well and 24-well size offers space to integrate a quartz crystal microbalance.

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7. Dec. 2012

Toxicological analysis of limonene reaction products using an in vitro exposure system

Toxicology in Vitro

2013 Mar;27(2):721-30. doi: 10.1016/j.tiv.2012.11.017. [Epub ahead of print]

Authors
Anderson SE, Khurshid SS, Meade BJ, Lukomska E, Wells JR.

Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, United States

 

Substitutional for indoor air chemistry, limonene and ozone shows alterations in inflammatory cytokine production on A549 cells and Mucil Air tissue.

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31. Mar. 2012

Inflammatory response modulation of airway epithelial cells exposed to formaldehyde

Toxicology Letters

2012, 211(2):159-163. doi:10.1016/j.toxlet.2012.03.799 [Epub ahead of print]

Authors
Persoz C, Achard S, Momas I, Seta N.

PRES Sorbonne Paris Cité, Université Paris Descartes, Laboratoire de Santé Publique et Environnement - EA 4064, Paris, France.

 

The comparing of the effects of formaldehyde on two cell lines, A549 and BEAS-2B, with the measured endpoints IL-8 and MCP-1. 

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31. Mar. 2012

New on SOT San Francisco

VITROCELL® 12/12 Module

The new module with 12 cell culture compartments is a compact solution for exposure using 12-well or 24-well sized inserts.

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Categories: Product News

Tags: VITROCELL 12

15. Mar. 2012

Impact of test conditions on in vitro cytotoxicity measurements of mainstream cigarette smoke using a whole smoke exposure system

SOT San Francisco, USA

March 11-15, 2012; Poster #1843

Authors
Xiang Li, Pingping Shang, Cong Nie, Fuwei Xie, Huimin Liu, Jianping Xie


Key Laboratory of Tobacco Chemistry, 

Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China

 

This article shows the influence of whole smoke on A549 cells under ISO and HC regimen. The results are shown on the NRU assay. 

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16. Dec. 2011

Effects of smoking regimens and test material format on the cytotoxicity of mainstream cigarette smoke

Food Chem Toxicol

2011 Dec 16; 50(3-4):545-551. [Epub ahead of print]

Authors
Li X, Shang P, Peng B, Nie C, Zhao L, Liu H, Xie J.

Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China

 

Total particulate matter or whole smoke generated from different test cigarettes under ISO or HCI regimens were assessed for cytotoxicity using the neutral red uptake cytotoxicity assay. Cytotoxicity among the test cigarettes were greater for whole smoke and the cytotoxicity was decreased going from ISO regimen to HCI regimen. 

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31. Aug. 2011

Effect of Formaldehyde On Corneal Epithelial Cells In An Air-Liquid Culture Model

EUROTOX Paris, France

August 28-31, 2011; Poster #P1198

Authors
Françoise Brignole-Baudouin1,2,4, Sophie Achard3, Charles Persoz3, Alice Emptoz1,2, Nathalie Seta3, Christophe Baudouin1,2,4

1 UMR_S968 INSERM UPMC; UMR 7210 CNRS; Institut de la Vision, Paris, France
2 Laboratoire de Toxicologie, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes
3 Laboratoire de Santé Publique et d’Environnement, EA 4064, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes
4 Centre Hospitalier National d’Ophtalmologie des Quinze-Vingts, 75012 Paris

 

The aim of this poster is to show the inflammatory effect of gaseous formaldehyde on human corneal epithelial cells in an air-liquid culture.The Results are shown in protein assay, LDH, IL-8, MCP-1 and flow cytometry assays.

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31. Aug. 2011

In vitro model of repeated exposure to different contaminants of human respiratory cells

EUROTOX Paris, France

August 28-31, 2011; Poster #P1361

Authors
Charles PERSOZ 1, Christopher LELEU 2, Sophie ACHARD 1, Magali FASSEU 3, Jean MENOTTI 2, Pascale MENECEUR 2, Francis DEROUIN 2, Nathalie SETA 1

1 Laboratoire Santé Publique et Environnement, EA4064,Université Paris Descartes, Paris, France
2 Laboratoire de Parasitologie-Mycologie, EA 3520, Université Paris Diderot, Paris, France
3 Centre de Recherche Biomédicale Bichat-Beaujon, Inserm, U773, Paris, France

 

The aim of this poster is to show the development of an in vitro model to exposure two different pollutants, a chemical pollutant (formaldehyde-FA) and a biological contaminant (Aspergillus fumigatus-Asp),were assessed in vitro on epithelial respiratory cells (A549).The results are shown in cellular viability (LDH assay), local inflammation, IL-8, MCP-1 and mRNA expression quantified by RT-PCR.

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