Advanced in vitro exposure systems.

22. Aug. 2011

Prävalidierungsstudie zur Prüfung der toxischen Wirkung von inhalativ wirksamen Stoffen (Gase)

Prevalidation study for testing toxic effects of inhalable substances (gases)

1. Auflage. Dortmund: Bundesanstalt für Arbeitsschutz und Arbeitsmedizin 2011

Authors
G. Linsel, M. Bauer, E. Berger-Preiß, C. Gräbsch, H. Kock, M. Liebsch, R. Pirow, D. Ritter, L. Smirnova, J. Knebel: ISBN: 978-3-88261-131-1, 43 Seiten, Projektnummer: F 1835, Papier, PDF-Datei

 

The aim of the study was the prevalidation of an inhalation toxicity test for gases using human lung cells exposed on the air liquid interface. Four gases, nitrogen dioxide (NO2), sulfur dioxide (SO2), formaldehyde, ozone as well as synthetic air for negative control were investigated and evaluated in cytotoxicity and genotoxicity endpoints. (german article)

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12. Aug. 2011

Ex vivo toxicity of nitrogen dioxide in human nasal epithelium at the WHO defined 1-h limit value

Toxicology Letters

2011 Nov 10;207(1):89-95. doi: 10.1016/j.toxlet.2011.08.004. [Epub ahead of print]

Authors
C. Koehler, C. Ginzkey, G. Friehs, S. Hackenberg, K. Froelich, A. Scherzed, M. Burghartz, M. Kessler, N. Kleinsasser

Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Wuerzburg, Germany

 

The present study focuses on toxic effects of NO₂. Nasal epithelial mucosa cells were cultured on the air-liquid interface. After exposure, analysis of genotoxicity was performed by the comet assay and by the micronucleus test. 

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5. Aug. 2011

Sequential air-liquid exposure of human respiratory cells to chemical and biological pollutants

Toxicology Letters

2011 Nov 10;207(1):53-9. doi: 10.1016/j.toxlet.2011.07.028 [Epub ahead of print]

Authors
Persoz C, Leleu C., Achard S., Fasseu M., Menotti J., Meneceur P., Momas I., Derouin F., Seta N. Source

Université Paris Descartes, Laboratoire de Santé Publique et Environnement - EA 4064, Paris, France

 

The aim of this study is to manage air-liquid successive exposures to different pollutants such as a chemical pollutant and a biological contaminant using human alveolar cells (A549), which were exposed at the air-liquid interface. Inflammation biomarkers (IL-8, MCP-1) were assayed by ELISA and by RT-PCR.

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10. Mar. 2011

In vitro exposure of organotypical 3D epithelial tissues to cigarette smoke as a potential alternative to rodent inhalation studies

SOT Washington DC, USA

March 06-10, 2011

Authors
Weisensee, D., Kurkowsky, B., Hebestreit, M., Wagner, S. and Schueller J. 

Philip Morris Research Laboratories GmbH, Cologne, Germany, Abstract #998

 

Here we describe an in vitro test system that examines the toxicological effects of single or repeated cigarette smoke exposure on human organo- typically reconstituted 3D epithelial tissues. These tissues were exposed at the air liquid interface to either fresh air or to mainstream smoke from the Reference Cigarette 3R4F in the VITROCELL® system. 

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30. Jul. 2010

New on IUTOX

VITROCELL® Microbalance

The ideal tool for monitoring of partcle deposition in the exposure module.

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30. Jun. 2010

Effects of acute in vitro exposure of murine precision-cut lung slices to gaseous nitrogen dioxide and ozone in an air-liquid interface (ALI) culture

Toxicology Letters

2010 Jul 1;196(2):117-24. doi: 10.1016/j.toxlet.2010.04.004. [epub ahead of print]

Authors
S. Switalla, J. Knebel, D. Ritter, N. Krug, A. Braun, K. Sewald∗

Fraunhofer Institute for Toxicology and Experimental Medicine, Nikolai-Fuchs-Str. 1, 30625 Hannover, Germany

 

The publications shows a direct exposure to O(3) and NO(2) induced cytotoxicity and pro-inflammatory responses in precision-cut lung slices in an air-liquid interface culture. This provides a model that more closely resembles in vivo exposure of airborne contaminants.

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10. Mar. 2010

An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells

Toxicology Letters

2010 Jun 2;195(2-3):99-105. doi: 10.1016/j.toxlet.2010.03.003. [epub ahead of print]

Authors
Persoz C., Achard S., Leleu C., Momas I., Seta N.

Université Paris Descartes, Laboratoire de Santé Publique et Environnement - EA 4064, Paris, France

 

This study was thus set up to design an in vitro model, using a direct exposure device to study the cellular effects of air pollutants at environmental doses on lung epithelial cells and apply this to gaseous formaldehyde. Therefore A549 cells were exposed using the air-liquid interface to formaldehyde. After exposure, cellular viability (XTT) and inflammation (IL-6, IL-8 and MCP-1) were assessed.

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7. Mar. 2010

Aspects of nitrogen dioxide toxicity in environmental urban concentrations in human nasal epithelium.

Toxicol Appl Pharmacol.

2010 Jun 1;245(2):219-25. doi: 10.1016/j.taap.2010.03.003. [Epub ahead of print]

Authors
Koehler C., Ginzkey C., Friehs G., Hackenberg S., Froelich K., Scherzed A., Burghartz M., Kessler M., Kleinsasser N.

Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Germany

 

Nasal epithelial cells of were cultured in an air-liquid interface and exposed to NO(2). After exposure, genotoxicity was evaluated by the Comet assay and the micronucleus test. Depression of proliferation and cytotoxic effects were determined using the micronucleus assay and trypan blue exclusion assay.

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16. Sep. 2009

Cytokine Production after Acute Exposure of Precision Cut Lung Slices to NO2 and O3

EUROTOX. Dresden, Germany.

September 13-16, 2009

Authors
K. Sewald, S. Switalla, D. Ritter, N. Krug, J. Knebel, A. Braun

Fraunhofer Institute for Toxicology and Environmental Medicine, Hannover, Germany

 

Precise cut lung slices were washed and exposed to synthetic air, O3 and NO2 in an air-liquid interface. Viability was controlled by Wst-1 and LIVE/DEAD® staining. Cytokines were quantified by ELISA or Luminex technology.

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16. Sep. 2009

An in vitro model to assess the impact on respiratory cells (A549) of air pollution

EUROTOX. Dresden, Germany.

September 13-16, 2009. Bo Holmstedt

Authors
Charles Persoz, Sophie Achard, Christopher Leleu, Isabelle Momas, Nathalie Seta

Laboratoire Sante Publique et Environment - EA 4064 - Universite Paris Descartes, France

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