Advanced in vitro exposure systems.

17. Jun. 2014

Development of a BALB/c 3T3 neutral red uptake cytotoxicity test using a mainstream cigarette smoke exposure system

BioMed Central

BMC Research Notes 2014, 7:367 (doi:10.1186/1756-0500-7-367)

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Linsey Haswell1, Annette Dalrymple1, Clive Meredith1 and Deborah Dillon1

1 British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

Mainstream cigarette smoke and the gase vapour phase, generated with the VC 10® smoke exposure system, indicates significant differently in-vitro cytotoxicity profiles. The used method on the air-liquid interface takes into account the synergies of both the particulate and vapour phase of tobacco smoke in the NRU assay.

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2. Jun. 2014

Application of a modified gaseous exposure system to the in vitro toxicological assessment of tobacco smoke toxicants

Environmental and Molecular Mutagenesis

Volume 55, Issue 8, Article first published online: 2 JUN 2014 (doi: 10.1002/em.21876)

Authors
Damien Breheny1, Fiona Cunningham1, Joanne Kilford2, Rebecca Payne2, Deborah Dillon1 and Clive Meredith1

1 British American Tobacco, Group R&D, Southampton, Hampshire, United Kingdom
2 Covance Laboratories Ltd, North Yorkshire, United Kingdom

Demonstration of the suitability of the VC 10® exposure system for the assesment of the mutagenic potential of gases using the AMES Assay. Not only cigarette smoke, also a wide range of gaseous compounds and aerosol mixtures can be studied.

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31. May. 2014

New VITROCELL® Automated Exposure Station delivered to NanoMile consortium

The NanoMILE project intends to establish a fundamental understanding of the mechanisms of nanomaterial interactions with living systems and the environment. It consists of 28 reseach partners. VITROCELL supllies the ALI technology.

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28. Apr. 2014

Efficient bioactive delivery of aerosolized drugs to human pulmonary epithelial cells cultured at air-liquid interface conditions

Am J Respir Cell Mol Biol

First published online 28 Apr 2014 (doi: 10.1165/rcmb.2013-0479OC)

Authors
Anke-Gabriele Lenz1, Tobias Stoeger1, Daniele Cei2, Martina Schmidmeir1, Nora Pfister1, Gerald Burgstaller1, Bernd Lentner1, Oliver Eickelberg3, Silke Meiners1 and Otmar Schmid1

1 Helmholtz Zentrum München, Institute of Lung Biology and Disease, Comprehensive Pneumology Center, Member of the German Center for Lung Research, Neuherberg/Munich, Germany
2 University of Pisa, Interdepartmental Research Center E.Piaggio”, Faculty of Engineering, Pisa, Italy
3,1 Ludwig-Maximilians-University, University Hospital, Munich, Germany

Introduction of the VITROCELL®-CLOUD (air-liquid interface cell exposure system-cloud) technology, which utilizes principles of cloud motion for fast and quantitative delivery of aerosolized liquid drugs to pulmonary cells cultured under realistic air-liquid interface conditions. These data indicate that the VITROCELL®-CLOUD is a reliable tool for aerosolized drug screening on cells.

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27. Mar. 2014

Potential toxicity of electronic cigarette liquids and aerosols as measured by four in vitro assays

SOT Phoenix, USA

24-27 March, 2014

Authors
Leverette, R.D., Misra, M., Cooper, B.T. and Bennett, M.B.

Lorillard Tobacco Company, A.W. Spears Research Center, Greensboro, NC 27405, USA

Exposition of tobacco and e-cigarette smoke, that were smoked on a VC 10® smoking robot in combination with a Vitrocell laser photometer for caculation of the delivered dose. The cytotoxicity was measured in four different endpoints: AMES, NRU, IL-8 Release and Micronucleus Assay.

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27. Mar. 2014

Cerium oxide nanoparticles air exposure: a comparison study using a human 3D airway model and A549 and Beas-2B cell lines

SOT Phoenix, USA

24-27 March, 2014

Authors
Ingeborg M. Kooter1, Mariska Grollers-Mulderij1, Maaike Steenhof1, Evert Duistermaat2, Frederique van Acker2, Yvonne Staal2, Eric Schoen1, Rob Stierum1, Linette Pellis1, Frieke Kuper1

1 The Netherlands Organisation for Applied Scientific Research, TNO, Zeist, The Netherlands
2 TNO Triskelion, Zeist, the Netherlands

Exposition of nano and macro particles on 3D lungcell models in comparison to normal lung cell lines in the air-liqid interface. Differentially effects are shown in the endpoints HO1, IL8, LDH, Comet Assay and gene expression.

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1. Nov. 2013

A review of in vitro cigarette smoke exposure systems

Experimental and Toxicologic Pathology

Volume 65, Issues 7-8, November 2013, Pages 1183-1193. pii: S0940-2993(13)00081-X. doi: 10.1016/j.etp.2013.06.001. [Epub ahead of print]

Authors
Thorne D, Adamson J.

British American Tobacco, Group R&D, Southampton SO15 8TL, UK.

In this review, the authors present a summary of the major tobacco smoke exposure systems available and critically review their function, set-up and application for in vitro exposure scenarios.

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3. Oct. 2013

A modified Ames methodology for the assessment of mainstream cigarette smoke genotoxicity using an aerosol-based exposure system

Poster CORESTA SSPT Meeting. Seville, Spain.

29 September - 3 October 2013

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Annette Dalrymple1, Julie Clements2, Clive Meredith1, Debbie Dillon1

1 British American Tobacco, Group R&D, Southampton, United Kingdom
2 Covance Laboratories Ltd, Harrogate, North Yorkshire, United Kingdom

 

The successfull exposure of whole smoke on a modified AMES assay using the the air-liquid interface exposure system. 

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30. Sep. 2013

Modelling and measurement of particle deposition for cell exposure at the air-liquid interface

Journal of Aerosol Science

Volume 63, September 2013, Pages 103-114

Authors
Andreas Comoutha, Harald Saathoffa, Karl-Heinz Naumanna, Sonja Muelhoptb, Hanns-Rudolf Paurb, Thomas Leisnera

a Institute for Meteorology and Climate Research, KIT, Karlsruhe, Germany
b Institute for Technical Chemistry, KIT, Karlsruhe, Germany

Presentation of a nanoparticle model to predict the size and particle deposition efficiencies for the air-liquid interface exposure system, that allows easy and accurate dose estimations. 

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30. Sep. 2013

In vitro reconstituted human airway epithelium model to assess the impact of indoor air pollutants on the inflammatory response

Poster EUROTOX 2013. Interlaken, Switzerland.

September 2013

Authors
Sophie Achard, Gaëlle Bardet, Sophie Grellet, Isabelle Momas, Nathalie Seta

Université Paris Descartes, PRES Cité Sorbonne, Faculté de Pharmacie; EA4064 Impact sanitaire des pollutions, 75006 Paris, France

 

The exposition of Formaldehyde as a major indoor air pollutant on a 3D nasal cell modell is demonstrated in the results if IL-8 and MCP assay.

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