Advanced in vitro exposure systems.

14. Mar. 2016

Cellular Effects in an In Vitro Human 3D Cellular Airway Model and A549/BEAS-2B In Vitro Cell Cultures Following Air Exposure to Cerium Oxide Particles at an Air–Liquid Interface

DOI: 10.1089/aivt.2015.0030

Ingeborg M. Kooter,1 Mariska Gröllers-Mulderij,1 Maaike Steenhof,1 Evert Duistermaat,2 Frederique A.A. van Acker,2 Yvonne C.M. Staal,2 Peter C. Tromp,1 Eric Schoen,1 C. Frieke Kuper,1 and Eugene van Someren1

1The Netherlands Organisation for Applied Scientific Research, TNO, Utrecht, The Netherlands.
2TNO Triskelion BV, Zeist, The Netherlands.

BEAS-2B, A549, and MucilAir cells were exposed to CeO2 particles and are cultured at air–liquid interface. LDH, cytokine analysis, gene expression analyses, HO-1 analyses and Comet assay were used for evaluation. Cell responses in the MucilAir model exposed via air to CeO2 were minimal compared to those of the cell lines.

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9. Mar. 2016

Toxicity testing of combustion aerosols at the air-liquid interface with a self-contained and easy-to-use exposure system

doi:10.1016/j.jaerosci.2016.02.005

Sonja Mülhopt a, i, Marco Dilger a, b, i, Silvia Diabaté b, i, Christoph Schlager a, i, Tobias Krebs c, i, Ralf Zimmermann d, h, i, Jeroen Buters e, i, Sebastian Oeder e, g, i, Thomas Wäscher f, Carsten Weiss b, i, Hanns-Rudolf Paur a, i

a Karlsruhe Institute of Technology, Institute for Technical Chemistry, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
b Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
c Vitrocell Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany
d University of Rostock, Institute of Chemistry, Dr.-Lorenz-Weg 1, 18051 Rostock, Germany
e Center of Allergy & Environment (ZAUM), Technische Universität and Helmholtz Zentrum München, Biedersteiner Str. 29, 80802 München, Germany
f Ingenieurbüro für Energie- und Verfahrenstechnik, Von-Dalheim-Str. 2, 69231 Rauenberg, Germany
g Kühne Foundation, Christine Kühne Center for Allergy Research and Education (CK- CARE), München, Germany
h Cooperation Group “Comprehensive Molecular Analytics” – CMA, Helmholtz Zentrum München, 85764 Oberschleißheim/ Germany
i HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health

 

Highlights

• We present a fully automated Air-Liquid-Interface Exposure System.
• System characterisation shows a high reproducibility.
• Wood combustion aerosol causes cytotoxicity in human lung epithelial cells.
• Gene regulation caused by ship diesel emissions in human lung epithelial cells.

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8. Jan. 2016

Carbonbeton – eine neue Art des Bauens

Projekt C³-Carbon Concrete Composite

VITROCELL® Systems GmbH ist Teil dieses größten Bauforschungsprojektes Deutschlands, dem Projekt C³-Carbon Concrete Composite. Zusammen mit mehr als 130 Partnern aus Forschung, Unternehmen und Verbänden wird im C³-Projekt einen neuer Materialverbund aus Carbonfasern und Hochleistungsbeton mit unserer Technologie getestet.

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31. Oct. 2015

Roflumilast partially reverses smoke-induced mucociliary dysfunction

DOI 10.1186/s12931-015-0294-3

Andreas Schmid, Nathalie Baumlin, Pedro Ivonnet, John S. Dennis, Michael Campos, Stefanie Krick and Matthias Salathe*

* Correspondence: msalathe@med.miami.edu
Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Miami Miller School of Medicine, 1600 NW 10th Ave, RMSB #7058, Miami, FL 33136, USA

 

Primary normal human bronchial epithelial cells (NHBE) from non-smokers, cultured at the air-liquid interface (ALI), were exposed to cigarette smoke in a Vitrocell VC-10 smoking robot. The results show that roflumilast can rescue smoke-induced mucociliary dysfunction.Therefor the ELIZA Assay and qPCR were used.

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29. Sep. 2015

Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure

DOI 10.1186/s12989-015-0104-6

Linda C. Stoehr1,2†, Carola Endes3†, Isabella Radauer-Preiml1, Matthew S. P. Boyles1, Eudald Casals4, Sandor Balog5, Markus Pesch2, Alke Petri-Fink3, Barbara Rothen-Rutishauser3, Martin Himly1, Martin J. D. Clift3 and Albert Duschl1*

1Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria.

2Grimm Aerosol Technik GmbH & Co. KG, Ainring, Germany.
3BioNanomaterials, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
4Institut Català de Nanotecnologia (ICN), Bellaterra, Spain.
5Soft Matter Scattering, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
* Correspondence: albert.duschl@sbg.ac.at


The use of fluorescence-based reporter cell lines can provide a useful tool in screening the pro-inflammatory response following NP exposure in the air-liquid interface. The results are shown in the IL-8 assay, qRT-PCR ans ELISA.

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12. Jul. 2015

Development of an in vitro cytotoxicity model for aerosol exposure using 3D reconstructed human airway tissue; application for assessment of e-cigarette aerosol

DOI:10.1016/j.tiv.2015.05.018

Authors

Louise Neilsona, Courtney Mankusb, David Thornea, George Jacksonb, Jason DeBayb, Clive Mereditha

a British American Tobacco, Group Research and Development, Regents Park Road, Southampton, Hampshire SO15 8TL, United Kingdom
b MatTek Corporation, 200 Homer Avenue, Ashland, MA 01721, United States

 

The presentation of a new highly differentiated 3D airway culture, grown and exposed in the VITROCELL® system, shows an successful compatibility of the tissue and the VITROCELL® system for testing e-cigarettes. The results are shown with the MTT assay for cell viability and measurement of TEER.

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27. Jun. 2015

Translocation of gold nanoparticles across the lung epithelial tissue barrier: Combining in vitro and in silico methods to substitute in vivo experiments

DOI 10.1186/s12989-015-0090-8

Gerald Bachler1,2, Sabrina Losert1,3, Yuki Umehara2, Natalie von Goetz1*, Laura Rodriguez-Lorenzo2, Alke Petri-Fink2,
Barbara Rothen-Rutishauser2 and Konrad Hungerbuehler1

1ETH Zürich, Institute for Chemical and Bioengineering, 8093 Zürich, Switzerland.
2University of Fribourg, Adolphe Merkle Institute, 1700 Fribourg, Switzerland.
3EMPA, Swiss Federal Laboratories for Material Science and Technology, 8600 Dübendorf, Switzerland.

* Correspondence: natalie.von.goetz@chem.ethz.ch

 

This replacement study for animal testings shows aerosolized nanoparticles tested on monolayer epithelial cultures in the air-liquid interface to determine their translocation kinetics across the epithelial tissue barrier.

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3. Jun. 2015

Particulate Matter from Both Heavy Fuel Oil and Diesel Fuel Shipping Emissions Show Strong Biological Effects on Human Lung Cells at Realistic and Comparable In Vitro Exposure Conditions

DOI: 10.1371/journal.pone.0126536

Authors

Sebastian Oeder , Tamara Kanashova , Olli Sippula , Sean C. Sapcariu , Thorsten Streibel , Jose Manuel Arteaga-Salas , Johannes Passig , Marco Dilger, Hanns-Rudolf Paur, Christoph Schlager, Sonja Mülhopt, Silvia Diabaté, Carsten Weiss, Benjamin Stengel, Rom Rabe, Horst Harndorf, Tiina Torvela, Jorma K. Jokiniemi, Maija-Riitta Hirvonen, Carsten Schmidt-Weber, Claudia Traidl-Hoffmann, Kelly A. BéruBé, Anna J. Wlodarczyk, Zoë Prytherch, Bernhard Michalke, Tobias Krebs, André S. H. Prévôt, Michael Kelbg, Josef Tiggesbäumker, Erwin Karg, Gert Jakobi, Sorana Scholtes, Jürgen Schnelle-Kreis, Jutta Lintelmann, Georg Matuschek, Martin Sklorz, Sophie Klingbeil, Jürgen Orasche, Patrick Richthammer, Laarnie Müller, Michael Elsasser, Ahmed Reda, Thomas Gröger, Benedikt Weggler, Theo Schwemer, Hendryk Czech, Christopher P. Rüger, Gülcin Abbaszade, Christian Radischat, Karsten Hiller, Jeroen T. M. Buters , Gunnar Dittmar , Ralf Zimmermann
Institutes

 

Exposition of Heavy Fuel Oil and Diesel Fuel Shipping Emissions on human lung cells in the air-liquid interface exposure system for advanced chemical analysis combined with transcriptional, proteomic and metabolomic profiling and isotope labelling methods.

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22. May. 2015

Testing particle delivery of e-cigarettes

VITROCELL® Application Note for VC 10® S-TYPE Smoking Machine and Particle Photometers

The new application note shows a successfull monitoring of the particle delivery of different e-cigarettes on the setup of a VITROCELL VC 10® S-TYPE Smoking Machine combined with the VITROCELL® Particle Photometer and Monitor Software.

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22. May. 2015

Testing of conventional and e-cigarettes: using EpiAirway and EpiAlveolar with the VITROCELL® in vitro Exposure System

VITROCELL® VC1 Smoking Machine and 12/6 CF Exposure Module at the Air/Liquid Interface.

New MatTek application note shows a state-of-the-art in vitro set up by VITROCELL® Systems for testing combustion cigarettes as well as electronic cigarettes with the endpoints toxicity, oxidative stress, TEER, beating cilia, gene expression and mucus secretion.

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