Advanced in vitro exposure systems.

12. Jun. 2019

Molecular Signature of Asthma-Enhanced Sensitivity to CuO Nanoparticle Aerosols from 3D Cell Model

DOI: 10.1021/acsnano.9b01823

Ingeborg Kooter1, Marit Ilves 2, Mariska Gröllers-Mulderij 1, Evert Duistermaat 3, Peter C. Tromp 1, Frieke Kuper 1, Pia Kinaret 4,5, Kai Savolainen 6, Dario Greco 4,5, Piia Karisola 2, Joseph Ndika 2, and Harri Alenius 2,7

1The Netherlands Organization for Applied Scientific Research, TNO, P.O. Box 80015, Utrecht 3584 CB, The Netherlands
2Human Microbiome Research, Faculty of Medicine, University of Helsinki, P.O. Box 21, Helsinki 00290, Finland
3Triskelion B.V., P.O. Box 844, Zeist 3704 HE, The Netherlands
4Faculty of Medicine and Life Sciences, University of Tampere, Tampere FI-33014, Finland
5Institute of Biotechnology, University of Helsinki, P.O. Box 56, Helsinki 00014, Finland
6Finnish Institute of Occupational Health, P.O. Box 40, Helsinki 00014, Finland
7Institute of Environmental Medicine, Karolinska Institutet, P.O. Box 210, Stockholm SE-17176, Sweden

3D human bronchial epithelial cells were cultured at the air−liquid interface that mimics relevant inhalatory exposure were exposed to aerosols of pristine (nCuO) and carboxylated (nCuOCOOH) copper oxide nanoparticles. This paper shows that the existence of asthma enhances sensitivity of the airways to nanoparticle aerosols, possibly as a combined result of a hyperactive airway and inefficient mucociliary clearance mechanisms in asthmatics. The test results are shown in cell viabilty (LDH), Inflammation (IL6, IL8, MCP1) and Transcroptomics.

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6. Jun. 2019

12th International Particle Toxicology Conference (IPTC 2019)

September 11–13, 2019. Salzburg, Austria

The IPTC series of conferences brings together experts on particle toxicology. The audience is worldwide and all types of particles are considered. The conference covers basic research issues, but has a strong focus on applications of particle toxicology, which are in work place safety, environmental safety, consumer safety, medical and diagnostic developments and other fields.

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Categories: Exhibitions

Tags: congress

7. Sep. 2019

Application of a multi‑layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e‑liquid and its corresponding aerosol using an e‑cigarette device

https://doi.org/10.1007/s00204-019-02565-9


Anita R. Iskandar, Filippo Zanetti, Diego Marescotti, Bjorn Titz, Alain Sewer, Athanasios Kondylis, Patrice Leroy, Vincenzo Belcastro, Laura Ortega Torres, Stefano Acali, Shoaib Majeed, Sandro Steiner, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Thomas Schneider, Stefan Frentzel, Florian Martin, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng


Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

Previous experimental setups shows the effects of e-liquids on cell viability (first layer), followed by investigating the potential mechanisms of toxicity elicited by e-liquids (second layer) and finally assessing the impacts of aerosols (third layer). In this present work shows how the three-layer framework is leveraged to evaluate the potential toxicity and biological effects of the MESH Classic Tobacco and Base e-liquids/aerosols compared with those of 3R4F CS.

 

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22. Aug. 2019

PETA International Science Group and Tobacco Companies Donate to Save Animals

PETA International Science Group and Tobacco Companies Donate to Save Animals

Gaithersburg, Md. – The PETA International Science Consortium Ltd., Imperial Brands PLC, Altria Client Services (ALCS), British American Tobacco (BAT) PLC, and Philip Morris International Inc have joined together to donate equipment that can help to replace the use of animals in respiratory testing with more human-relevant, non-animal test methods.

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Categories: Publications

Tags: VITROCELL 12

11. Aug. 2019

Exposure to aerosols from electronic cigarettes using the MESH™ technology has a reduced biological impact on bronchial epithelial cell cultures compared with exposure to cigarette smoke

Gordon Research Conference, Integration of Emerging Technologies in Mechanistic and Translational Toxicology,Andover, August 11–16, 2019

Albert Giralt, Florian Martin, Anita R. Iskandar, Alain Sewer, Laura Ortega Torres, AthanasiosKondylis, Patrice Leroy, Celine Merg, ShoaibMajeed, Emmanuel Guedj, Thomas Schneider, KeyurTrivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng


PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud5, CH-2000 Neuchâtel, Switzerland
 

In contrast to 3R4F CS exposure, exposure to IQOS MESH™ Classic Tobacco aerosols did not cause tissue damage or have an impact on ciliary beating functionality in bronchial epithelial cell cultures despite resulting in greater concentrations of deposited nicotine. Cultures exposed to IQOS MESH™ Classic Tobacco aerosols showed fewer changes in proteins involved in xenobiotic metabolism than those exposed to CS.

 

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26. Jun. 2019

PETA Science Group Donates $50,000 in Equipment to Pioneering Laboratory

The Institute for In Vitro Sciences Will Use the Donation to Replace Animals in Respiratory Toxicity Testing

The PETA International Science Consortium Ltd. is donating $50,000 in equipment to the Institute for In Vitro Sciences (IIVS), a nonprofit laboratory in Gaithersburg that conducts and develops animal-free test methods. IIVS will use the VITROCELL® Cloud inhalation exposure system—instead of animals—to assess the effects of substances on the human respiratory tract.

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Categories: Publications

Tags: VITROCELL Cloud

29. May. 2019

55th Congress of the European Societies of Toxicology (EUROTOX)

September 8–11, 2019. Helsinki, Finland

The congress will offer a change to tackle with the burning chemical safety challenges of the next decade. The theme of the Congress is “Toxicology – Science Providing Solutions”.

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27. May. 2019

Characterization of the Vitrocell® Cloud SEQ 24 System and its Application in Exposure to Aerosolized Bortezomib and Ibuprofen

S. Steiner1, Sandra Ferreira1, Moran Morelli1, Audrey Baldi1, Arkadiusz K. Kuczaj1, Stefan Frentzel1, Marco van der Toorn1, Manuel Peitsch1, Julia Hoeng1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

This poster shows the investigation how aerosols are delivered within the Cloud SEQ 24 and how cell cultures respond to exposures in the system. Ibuprofen and Bortezomib was exposed to three dimensional organotypic tissue cultures of the human bronchial epithelium and biological endpoints were measured: Cytotoxicity, Interleukin 8 and Proteasome activity.

 

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25. May. 2019

In vitro model for the prediction of respiratory sensitization of inhaled chemicals and protein allergens

ISAM, Montreux, 25 – 29 May 2019

Weber P. 1, Chary A.1,2, Saibene M. 1, Serchi T.1, Cambier S.1 , Moschini E.1, Contal S.1, Hennen J.2, Ezendam J.3, Blömeke B.2, Gutleb A.C.1
1 Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Luxembourg. 
2 Department of Environmental Toxicology, Trier University, Germany.
3Centre for Environmental Protection, National Institute for Public Health and the Environment (RIVM), The Netherlands.

The Aim of this study is the adaptation of a 3D-co-culture system previously developed by Klein et al.2013. Inclusion of dendritic cells to study respiratory sensitization processes and identification and evaluation of markers to assess the respiratory sensitizing potential of inhaled compounds.

 

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Categories: Publications

Tags: VITROCELL Cloud

29. Apr. 2019

ISAM-Congress

May 25–29, 2019. Montreux, Switzerland

The congress will bring together about 400 international attendees at one of the largest pulmonary drug delivery and respiratory health conferences.

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