VITROCELL presents the direct exposure technology for the in vitro analysis of airborne substances such as gases, nano particles and complex mixtures.

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Vol 177 (Abstracts Issue): pA728 (2008)

Authors
Walsh, P.T., Chalupowicz, D.G., Frankowski, R., Barnette, M.

Discovery Biology, Respiratory CEDD, GlaxoSmithKline, Upper Merion, PA19406, USA

HBE cells were exposed in a single and repeated whole smoke situation. The cells were analysed for changes in gene expression (RT-PCR) and cell viability was confirmed using the MTT assay and release of IL-1, IL-6 and IL-8.

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October 2007, pp 402-414

Authors
Sonja Mülhopt, Hanns-Rudolf Paur, Silvia Diabaté and Harald F. Krug

Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein - Leopoldshafen, Germany

Exposure experiments with the fine and ultrafine fraction of fly ash on human lung cells in the air-liquid interface have shown an increased release of IL-8. 

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October 7-10, 2007

Authors
Letizia Farmer, Alexander Graff, Sandra Szameit, Eva Valic, Helga Tuschl

Austrian Research Centers GmbH - ARC, Life Sciences, Toxicology, A-2444 Seibersdorf, Austria

Differentiated macrophages were seeded on human lung epithelial cells for co-culturing. As endpoints for particle exposure cell viability (WST-1 assay), oxidative stress (DHRAssay) and pro-inflammatory cytokines (BDTM CBA-Assay) were evaluated.

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Experimental and Toxicologic Pathology 58 (2007) 383-392

Authors
Michaela Aufderheide, Helga Gressmann

Department of In-Vitro Toxicology, Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany

Establishment of a new experimental approach for direct exposure of bacteria with actual generated smoke aerosol. This allowed us to investigate the mutagenic activity of native mainstream smoke on Salmonella Typhimurium strains. The new procedure of exposing bacteria directly to gases and complex mixtures offers new possibilities in the field of inhalation genotoxicology for the evaluation of genotoxicity in the Ames assay.

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