Schmid O1,2, Jud C3, Umehara Y3, Mueller D4, Bucholski A4, Gruber F4, Denk O4, Egle R4, Petri-Fink A3, Rothen-Rutishauser B3.
1 Comprehensive Pneumology Center (CPC), German Center for Lung Research (DZL) , Munich, Germany .
2 Institute of Lung Biology and Disease, Helmholtz Zentrum München-German Research Center for Environmental Health , Neuherberg, Germany .
3 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg, Switzerland .
4 PARI Pharma GmbH, Starnberg, Germany .
The lung epithelial barrier was studied in vitro. Human lung epithelial cells (alveolar A549 and bronchial 16HBE14o- epithelial cells) were exposed to an aerosolized immunosuppressive drug encapsulated in liposomes at the air-liquid interface (ALI) using a dose-controlled air-liquid interface cell exposure (ALICE) system.