Tobias Krebs

VITROCELL Systems GmbH, 79183 Waldkirch, Germany 

The poster shows the VITROCEL® 48 exposure system with a 6- and a 24-well setup.

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Katharina Knoth¹, Marius Hittinger¹, Tobias Krebs²

¹PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany  

²VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The poster shows two customer examples for exposures to smallest quantities of dry powders.

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Markus Berger, Marco Hebestreit

VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The Poster shows a demonstration of the VC 10 smoking machine with Shisha option

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Chary A.^1,2, Serchi T.1, Cambier S.1, Moschini E.1, Contal S.¹, Hennen J.², Ezendam J.³, Blömeke B.², Gutleb A.C.^1
1 Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology (LIST), Luxembourg.
² Department of Environmental Toxicology, Trier University, Germany. 3Centre for Environmental Protection,National Institute for Public Health and the Environment (RIVM), The Netherlands.

Aim
- Adapt a 3D-coculture system previously developed by us (Klein et al. 2013) allowing the study of respiratory sensitization processes by including dendritic cells, which have a crucial role in sensitization
- Find markers for the assessment of the respiratory sensitizing potential of inhaled compounds by measuring the activation of DCs as well as the release of cytokines

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Lukasz Czekala¹, Matthew Stevenson¹, Liam Simms¹, Nicole Tschierske¹, Anna G. Maione², Tanvir Walele^3
1 Imperial Tobacco Ltd, 121 Winterstoke Road, Bristol, BS3 2LL UK
² MatTek, 200 Homer Ave., Ashland, MA, USA
³ Fontem Ventures B.V., an Imperial Brands PLC Company, Radarweg60, 1043 NT Amsterdam

A 3D in vitro organotypic model of the human respiratory epithelium was cultured at the Air-Liquid Interface (ALI) and exposed to cigarette smoke and e-cigarette aerosol, generated by using a VITROCELL® VC1 smoking machine under Health Canada Intense and CORESTA conditions.
The results are shown in tissue viability (MTT), barrier integrity (TEER), Barrier function, tissue secretion of the pro-inflammatory cytokines (IL-6 and IL-8). The presence of 8-isoprostane is used as an indicator of oxidative stress and antioxidant deficiency. Additionally the tissue morphology was assessed by H&E staining and immunofluorescent staining was conducted for specific markers.

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