Advanced in vitro exposure systems.

25. May. 2019

In vitro model for the prediction of respiratory sensitization of inhaled chemicals and protein allergens

ISAM, Montreux, 25 – 29 May 2019

Weber P. 1, Chary A.1,2, Saibene M. 1, Serchi T.1, Cambier S.1 , Moschini E.1, Contal S.1, Hennen J.2, Ezendam J.3, Blömeke B.2, Gutleb A.C.1
1 Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Luxembourg. 
2 Department of Environmental Toxicology, Trier University, Germany.
3Centre for Environmental Protection, National Institute for Public Health and the Environment (RIVM), The Netherlands.

The Aim of this study is the adaptation of a 3D-co-culture system previously developed by Klein et al.2013. Inclusion of dendritic cells to study respiratory sensitization processes and identification and evaluation of markers to assess the respiratory sensitizing potential of inhaled compounds.

 

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Categories: Publications

Tags: VITROCELL Cloud

29. Apr. 2019

ISAM-Congress

May 25–29, 2019. Montreux, Switzerland

The congress will bring together about 400 international attendees at one of the largest pulmonary drug delivery and respiratory health conferences.

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Categories: Exhibitions

Tags: congress

29. Apr. 2019

IEHB Conference

May 10-12, 2019. Nanjing, China

This conference will provide a great platform to communicate with academic experts, industrial leaders, media giants, and regulation officials. Please join us in Nanjing for better indoor environemnt!

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Categories: Exhibitions

Tags: congress

5. Apr. 2019

Assessing the respiratory toxicity of dihydroxyacetone using an in vitro human airway epithelial tissue model

https://doi.org/10.1016/j.tiv.2019.04.007
Yiying Wanga, Qiangen Wub, Levan Muskhelishvilic, Kelly Davisc, Matthew Bryantb, Xuefei Caoa
a Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
b Division of Biochemistry Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
c Toxicologic Pathology Associates, Jefferson, AR 72079, United States of America

Unintentional exposure of respiratory mucosal surfaces to DHA aerosols is highly possible during sunless tanning sessions, a possibility for which it is not approved. In this study, the potential respiratory toxicity of DHA using the ALI airway tissue model is evaluated. To mimic in vivo exposure conditions, a liquid aerosol generation and exposure system was used and exposed ALI cultures to a range of non-cytotoxic doses of DHA aerosols at the air-liquid interface.


 

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22. Mar. 2019

In vitro toxicological evaluation of emissions from catalytic oxidation removal of industrial VOCs by air/liquid interface (ALI) exposure system in repeated mode.

DOI: 10.1016/j.tiv.2019.03.030
Méausoone C1, El Khawaja R1, Tremolet G1, Siffert S1, Cousin R1, Cazier F2, Billet S1, Courcot D3, Landkocz Y1.
1 UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.
2 Centre Commun de Mesure, Université du Littoral Côte d'Opale, Dunkerque, France.
3 UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.. Electronic address: dominique.courcot@univ-littoral.fr.

BEAS-2B cells were exposed using an Air-Liquid Interface (ALI) System (Vitrocell®) to gaseous flows of toluene vapors and outflow after catalytic oxidation of toluene. After exposure to gaseous flow, cytotoxicity, inflammatory response and Xenobiotic Metabolism Enzymes (XME) gene expression were investigated.

Highlights
• Toxicity of toluene increases with time when cells are repeatedly exposed.
• Toxicological analysis helps for validation of catalysts used in treatment of VOCs.
• Cellular response supports the identification of chemically undetected by-products.


 

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10. Mar. 2019

New Products at VITROCELL® User Group Meeting 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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10. Mar. 2019

Exposure to an aerosol from a novel electronic cigarette using the MESH™ technology elicited reduced biological impacts than exposure to cigarette smoke on buccal and small airway epithelial cultures

Society of Toxicology, Annual Meeting 2019, Baltimore, MD, USA | 10-14 March

Anita R. Iskandar, Filippo Zanetti, Athanasios Kondylis, Florian Martin, Alain Sewer, Laura Ortega Torres, Shoaib Majeed, Sandro Steiner, Emmanuel Guedj, Celine Merg, Thomas Schneider, Keyur Trivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng


PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

 

The impacts of an acute exposure to cigarette smoke (CS) and to aerosol from a novel electronic cigarette (EC) device using MESH™ technology were assessed using human organotypic buccal epithelial cultures and small airway epithelial cultures. A paired design was implemented: in parallel to the exposure to CS or EC aerosol, cultures were also exposed to air in the same exposure module. Tissue damage was not seen in cultures exposed to the IQOS MESH™ Classic Tobacco aerosol despite resulting in greater concentrations of deposited nicotine. In buccal cultures, CS and IQOS MESH™ Classic Tobacco aerosol elicited different infammatory response.

 

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6. Mar. 2019

Carbendazim induces death in alveolar epithelial cells: A comparison between submerged and at the air-liquid interface cell culture.

DOI: 10.1016/j.tiv.2019.03.004

Tollstadius BF, da Silva ACG, Oliveira BC, Pedralli, Valadares MC.

Laboratory of Education and Research in In vitro Toxicology, Faculty of Pharmacy, Universidade Federal de Goiás, Goiânia, GO, Brazil.

In this work, the toxicity of the fungicide Carbendazim upon A549 alveolar cells was tested in comparison of monolayer and air-liquid interface cell system exposition. 


 

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5. Mar. 2019

A lower impact of an acute exposure to electronic cigarette aerosols than to cigarette smoke in human organotypic buccal and small airway cultures was demonstrated using systems toxicology assessment

DOI:10.1007/s11739-019-02055-x
Filippo Zanetti1, Athanasios Kondylis1, Florian Martin1, Patrice Leroy1, Shoaib Majeed1, Sandro Steiner1, Yang Xiang1, Laura Ortega Torres1, Keyur Trivedi1, Emmanuel Guedj1, Celine Merg1, Stefan Frentzel1, Nikolai V. Ivanov1, Utkarsh Doshi2, Kyeonghee Monica Lee2, Willie J. McKinneyJr2, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
2 Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

 

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20. Feb. 2019

An in vitro coculture system for the detection of sensitization following aerosol exposure

DOI:10.14573/altex.1901241

Aline Chary1,2, Tommaso Serchi1, Elisa Moschini1, Jennifer Hennen2, Sébastien Cambier1, Janine Ezendam3, Brunhilde Blömeke2 and Arno C. Gutleb1
1Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Luxembourg; 
2Department of Environmental Toxicology, University Trier, Germany; 
3Centre for Health Protection, National Institute for Public Health and the Environment, The Netherlands

 

The Vitrocell® Cloud-6 system was used for the exposure of a coculture in vitro model to test compounds and vehicle controls. The 3D in vitro model was cultured at the air liquid interface consists of A549, EA.hy926, PMA-differentiated THP-1 and non-differentiated THP-1 cells, that were exposed to nebulized chemical respiratory sensitizers. The results are shown in flow cytometry measurements, cytokine release and gene expression.

 

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