Monitoring diesel exhaust particles deposition on lung cells in vitro by lock-in thermography

August 26, 2023

ISAM Congress 2023 – August 26-30, Saarbrücken, Germany

Ruiwen He a, Olivier Schaub c, Christoph Geers c, Gowsinth Gunasingam a, Maik Schultheiß d,
Bastian Gutmann d, Tobias Krebs d, Alke Petri-Fink a,b, Barbara Rothen-Rutishauser a
a Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
b Chemistry Department, University of Fribourg, Fribourg, Switzerland
c NanoLockin GmbH, Fribourg, Switzerland
d Vitrocell® Systems GmbH, Waldkirch, Germany

Diesel exhaust particles (DEPs) can deposit onto the respiratory epithelial surface upon inhalation exposure. Cellular burden of DEPs is important for dose-response relationships in the cells, however, non-invasive methods to continuously monitor DEPs on cells during exposure are still challenging and not well established.
The combination of the Vitrocell® Cloud Alpha system and the Calorsito mini integrates the cell exposure to DEPs and the detection of the exposed DEPs on the cells.
Our results suggest the potential of this combined system for continuously quantifying DEPs on/in the cells during exposure.

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