Method Development to Establish and Characterize an Air Liquid Interface (ALI) Exposure System Using Perfluorooctanoic Acid (PFOA) Liquid Aerosol

April 6, 2023

SOT 62nd Annual Meeting and ToxExpo, March 19-23 2023, Nashville, Tennessee, USA

Amit Gupta, Bob Moyer, Stephanie Pearson, William Puerner, Janae Newswanger, Steve Behringer, Cassady Griffin and Ed Psurny
Battelle Memorial Institute, Columbus, OH, USA

There has been an accelerating trend to shift from traditional in vivo inhalation toxicology studies to the development of alternate animal models (e.g., in vitro inhalation exposure systems). A major caveat in using such in vitro inhalation systems is that the controlled delivery and accurate characterization of exposure atmosphere is extremely challenging. The goal of this study was to establish consistent, reproducible exposures using a liquid aerosol and collect preliminary biological data following exposure of 3D normal human bronchial epithelial (NHBE) cell cultures (EpiAirway,MatTek, Ashland, MA). A VITROCELL® 24/48 exposure system (VITROCELL Systems GmbH, Germany), was used to conduct air liquid interface (ALI) exposuresof cell cultures. Perfluorooctanoic acid (PFOA) was used as a representative test compound for producing liquid aerosol. Methods were developed to generate and characterize inhalation exposure atmosphere in ALI exposure system.

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