Advanced in vitro exposure systems.

Lung toxicity determination by in vitro exposure at the air-liquid interface with an integrated online dose measurement

23. Mar. 2009

Nanosafe 2008: International Conference on Safe production and use of nanomaterials

Journal of Physics: Conference Series 170 (2009) 012008 (doi:10.1088/1742-6596/170/1/012008)

Sonja Mülhopt, S. Diabaté, T. Krebs, C. Weiss and H.-R. Paur

Institute for Technical Chemistry, Thermal Waste Treatment Division, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
VITROCELL Systems GmbH, 79183, Waldkirch, Germany


Human lung cell cultures are exposed to airborne nanoparticles in VITROCELL®system modules. After exposure the cells are analyzed to measure the biological responses of the viability (LDH, AlamarBlue) as well as the release of IL-8. Using fluorescein sodium particles shows a very good correlation between the sensor signal of the quartz crystal microbalance and the deposited mass on the membranes shown by spectroscopy. This system for the first time provides an online dose measurement for in vitro experiments with nanoparticles. 

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