Poster, SOT 63rd Annual Meeting and ToxExpo, March 10–14, 2024, Salt Lake City, Utah.
Jeremie Gafner , Christelle Lamboley , Jimmy Vernaz , Samuel constant , Louise Neilson
JT International SA, Scientific & Regulatory Affairs, Geneva, Switzerland Epithelix, 18, Chemin des Aulx − CH-1228 Plan-les-Ouates − Geneva − Switzerland
- Cinnamaldehyde, a natural compound found in cinnamon, is a flavoring agent that can cause skin and eye irritation according to ECHA C&L inventory, and respiratory irritation according to the FDA.
- 3D reconstructed human airway tissues (MucilAir™) were exposed to waterpipe aerosol containing different concentrations of cinnamaldehyde, using an air-liquid interface (ALI) exposure and aerosol generation system (Vitrocell®).
- Fluorescence was used to measure the aerosol deposition on the tissues.
- Following a 4-day repeated exposure to blends with varying conentrations of cinnamaldehyde, a dose-dependent decrease in cell viability, barrier integrity, cilia beat frequency, and inflammation was observed.
- The highest concentration of cinnamaldehyde (10,000 ppm) caused a 73% reduction in cell viability and a significant loss of barrier function, as indicated by the transepithelial electrical resistance (TEER) and the inflammatory marker IL-8.
- Cinnamaldehyde has a dose-dependent effect on human airway tissues, and the findings can complement the toxicological risk assessment of waterpipe additives, as well as provide insights into the mechanisms of irritation.
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