Investigation on the biological effects of 3D reconstructed human airway tissue exposed at the air-liquid interface to aerosols generated from waterpipe tobacco blends with varying concentrations of cinnamaldehyde

March 11, 2024

Poster, SOT 63rd Annual Meeting and ToxExpo, March 10–14, 2024, Salt Lake City, Utah.

Jeremie Gafner , Christelle Lamboley , Jimmy Vernaz , Samuel constant , Louise Neilson
JT International SA, Scientific & Regulatory Affairs, Geneva, Switzerland Epithelix, 18, Chemin des Aulx − CH-1228 Plan-les-Ouates − Geneva − Switzerland

  • Cinnamaldehyde, a natural compound found in cinnamon, is a flavoring agent that can cause skin and eye irritation according to ECHA C&L inventory, and respiratory irritation according to the FDA.
  • 3D reconstructed human airway tissues (MucilAir™) were exposed to waterpipe aerosol containing different concentrations of cinnamaldehyde, using an air-liquid interface (ALI) exposure and aerosol generation system (Vitrocell®).
  • Fluorescence was used to measure the aerosol deposition on the tissues.
  • Following a 4-day repeated exposure to blends with varying conentrations of cinnamaldehyde, a dose-dependent decrease in cell viability, barrier integrity, cilia beat frequency, and inflammation was observed.
  • The highest concentration of cinnamaldehyde (10,000 ppm) caused a 73% reduction in cell viability and a significant loss of barrier function, as indicated by the transepithelial electrical resistance (TEER) and the inflammatory marker IL-8.
  • Cinnamaldehyde has a dose-dependent effect on human airway tissues, and the findings can complement the toxicological risk assessment of waterpipe additives, as well as provide insights into the mechanisms of irritation.

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