In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

July 21, 2014

informa healthcare, posted online on July 21, 2014. (doi:10.3109/15376516.2014.943441)

Walter K. Schlage*, Anita R. Iskandar*, Radina Kostadinova*, Yang Xiang*, Alain Sewer*, Shoaib Majeed,
Diana Kuehn, Stefan Frentzel, Marja Talikka, Marcel Geertz, Carole Mathis, Nikolai Ivanov, Julia Hoeng,
and Manuel C. Peitsch

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel,

*Equal contribution

Exposition of cigarette smoke to tissue cultures at the air–liquid interface shows in combination of the microarray technology, gene-set analysis and a novel computational modeling an increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity. The usefulness of in vitro exposure to buccal and gingival organotypic epithelial tissue cultures for the impact assessment of cigarette smoke is demonstrated. 

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