In vitro model of repeated exposure to different contaminants of human respiratory cells

August 31, 2011

August 28-31, 2011; Poster #P1361

Charles PERSOZ 1, Christopher LELEU 2, Sophie ACHARD 1, Magali FASSEU 3, Jean MENOTTI 2, Pascale MENECEUR 2, Francis DEROUIN 2, Nathalie SETA 1

1 Laboratoire Santé Publique et Environnement, EA4064,Université Paris Descartes, Paris, France
2 Laboratoire de Parasitologie-Mycologie, EA 3520, Université Paris Diderot, Paris, France
3 Centre de Recherche Biomédicale Bichat-Beaujon, Inserm, U773, Paris, France


The aim of this poster is to show the development of an in vitro model to exposure two different pollutants, a chemical pollutant (formaldehyde-FA) and a biological contaminant (Aspergillus fumigatus-Asp),were assessed in vitro on epithelial respiratory cells (A549).The results are shown in cellular viability (LDH assay), local inflammation, IL-8, MCP-1 and mRNA expression quantified by RT-PCR.

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