In vitro exposure of isolated cells to native gaseous compounds – Development and validation of an optimized system for human lung cells

August 1, 2001

Exp Toxic Pathol 2001; 53: 373-386 accepted August 2, 2001

Detlef Ritter, Jan W. Knebel, and Michaela Aufderheide


Exposures of human lung fibroblasts (Lk004 cells) and human lung epithelial cells (HFBE-21 cells) to synthetic air, ozone and nitrogen dioxide established that cells could be treated long enough to detect biological effects of environmentally relevant gas mixtures. The analysis of viability (viable cell number, tetrazoliumsalt cleavage) and intracellular end-points (oxidized/reduced glutathione, ATP/ADP) showed that both gases induced relevant cellular changes. In exploratory experiments without cells, the reproducibility and quality of the gas/membrane contact in the air-liquid interface could be demonstrated.

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