jove, 12 Februray 2015, Issue 96 (doi: 10.3791/52325)
Authors
Diana Kuehn1, Shoaib Majeed1, Emmanuel Guedj1, Remi Dulize1, Karine Baumer1, Anita Iskandar1, Stephanie Boue1, Florian Martin1, Radina Kostadinova1, Carole Mathis1, Nikolai V. Ivanov1, Stefan Frentzel1, Julia Hoeng1, Manuel C. Peitsch1
1 Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A
The aim of this video protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke at the air-liquid interface to measure ciliary beating and TEER.