Dynamic in-vitro Exposure of Human Derived Cells to Indoor Priority Pollutants

December 31, 2006

Report EUR 22285 EN, 2006

F. Pariselli, M. G. Sacco, D. Rembges

Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, Ispra, Italy


The aim of this work was to investigate toxicity of single volatile chemicals and their mixtures. Developing a reproducible in vitro dynamic exposure model where A549 and HaCaT cell lines representative of VOC’s target tissues (lungs and skin) were exposed in an airlifted interface to air pollutants. Several endpoints were evaluated: cytotoxicity (LDH), proliferation (WST-1), CFU (Colony Forming Units), inflammatory response (IL-8) and oxidative stress (ROS).

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