Cellular Mechanisms of Mainstream Cigarette Smoke-Induced Lung Epithelial Tight Junction Permeability Changes In Vitro

March 23, 2007

Inhalation Toxicology, 19:13–22, 2007

Dorian S. Olivera
Program in Toxicology, College of Pharmacy, University of New Mexico Health Sciences Center,
Albuquerque, New Mexico, and Lovelace Respiratory Research, Albuquerque, New Mexico, USA

Susan E. Boggs, Chris Beenhouwer, James Aden, and Cindy Knall
Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA


The goal of these studies was to identify the signal transduction pathways that regulate smoke-induced permeability. Using a physiologically relevant air-liquid interface exposure system, electrically tight monolayers of Calu-3 cell line were exposed to fresh, whole mainstream cigarette smoke. This exposure results in a regulated, dose-dependent loss of epithelial barrier function in the lung epithelial monolayers.

read article

News overview