A novel in vitro micronucleus (MN) test procedure after exposure of human derived lung cells to volatile chemicals at the air/liquid interface in vitro

March 25, 2010

March 23-25, 2010

Richard Gminski, Christoph Modest, Benedikt Armbruster, Tao Tang, Mathias Könczöl, Volker Mersch-Sundermann

Department of Environmental Health Sciences Institute of Environmental Medicine and Hospital Hygiene (IUK), Freiburg University Medical Centre / Germany


To develop a new bioassay for the air-liquid interface system, they optimized the conditions for the cytochalasin-block in vitro micronucleus test procedure using commercially available PTFE cell culture inserts. After exposure, cells were evaluated for cell viability (WST-1 assay) and for the induction of micronuclei.

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