A modified Ames assay reveals the mutagenicity of native cigarette mainstream smoke and its gas vapour phase

July 23, 2007

Experimental and Toxicologic Pathology 58 (2007) 383-392

Michaela Aufderheide, Helga Gressmann

Department of In-Vitro Toxicology, Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany


Establishment of a new experimental approach for direct exposure of bacteria with actual generated smoke aerosol. This allowed us to investigate the mutagenic activity of native mainstream smoke on Salmonella Typhimurium strains. The new procedure of exposing bacteria directly to gases and complex mixtures offers new possibilities in the field of inhalation genotoxicology for the evaluation of genotoxicity in the Ames assay.

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