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VITROCELL® Exposure at the Air/Liquid Interface

The exposure of mammalian cells or tissues to airborne substances is frequently performed under submerged conditions. In doing so, the test substances are dosed into the culture medium. This procedure results in an undesired interaction of the formerly airborne substances with the medium, causing limitations for authentic analysis.

VITROCELL® Flow Illustration

In response to the scientific need to expose in physiologically relevant conditions, VITROCELL®s systems have been specifically designed and engineered to enable direct exposure of mammalian cells or tissue at the air/liquid interface where the cell systems are not covered with culture medium. Researchers can thus use all cell types cultivated on microporous membranes. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.


Submerged Cultivation and Exposure in Incubator	Suspension Cultivation and Exposure in Incubator	Air/Liquid Cultivation and Exposure in Exposure Module
A Medium above cells B Cells on membrane C Medium below cells	A Cells in medium	A Direct and controlled exposure of test atmosphere to cells B Cells on membrane C Medium below cells
Interaction of test components with culture medium Low sensitivity	Interaction of test components with culture medium Low sensitivity	No losses No reaction of principle components with culture medium High sensitivity of system

VITROCELL® Exposure at the Air/Liquid Interface

VITROCELL® Flow Illustration

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