Advanced in vitro exposure systems.

Copper oxide nanoparticles: Impact on alveolar epithelial-like cells following air-liquid interface exposure

14. Aug. 2016

EEMGS Annual Meeting: 14 – 18 August 2016, Copenhagen, Denmark

doi:10.1007/s00204-015-1621-7

Matthias Hufnagel, Sarah Schoch, Bettina Fischer, Andrea Hartwig
Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Food Chemistry and Toxicology

This is work is part of ProCycle (No. 03XP0009), a project funded by the German Federal Ministry of Education and Research.and shows the exposition of copper oxide (CuO) nanoparticles on A549 cells at different doses in the VITROCELL® Cloud, equipped with a quartz crystal microbalance. The effects were determined using the colony formation assay (CFA) and high-throughput RT-qPCR gene expression analyses.

Introduction
The increased use of nanomaterials is the driving force for increasing research on nanotoxicology. In contrast to the traditionally used submerged application, we used an innovative exposure method (air-liquid interface – ALI) which represents a more realistic exposure scenario for investigating airborne nanoparticles (NP). Additionally, the use of ALI exposure systems, e.g. VITROCELL® Cloud, enables the measurement of actual particle depositions, when equipped with a quartz crystal microbalance Within this study, adenocarcinoma human alveolar epithelial-like cells (A549) were exposed to copper oxide (CuO) NP at different doses in the VITROCELL® Cloud. Subsequently, cytotoxic effects were determined using the colony formation assay (CFA). Furthermore, the impact of CuO NP on genomic stability was investigated via high-throughput RT-qPCR gene expression analyses.

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