Poster, SOT 65th Annual Meeting and ToxExpo, March 22–25, 2026, in San Diego, California.
Jo Wallace1, Mary McElroy1, Blane Stobbs1, Anna Marshall1, James Baily1, Rossana Boni1, Mitch Klausner2, Seyoum Ayehunie2
1: Charles River Laboratories, UK. 2: MatTek , USA
Introduction
This study presents a comprehensive and systematic comparison of ten chemicals using advanced human airway models. A key aspect of the work is the inter-laboratory comparison between two independent labs, demonstrating the robustness and reproducibility of the approach.
In addition, the study investigates the impact of exposure methodology by directly comparing aerosolized delivery with liquid application at the air-liquid interface (ALI). This provides valuable insight into how different dosing routes influence toxicological outcomes.
One of the most important findings is the strong predictive performance of the in vitro models in relation to Globally Harmonized System (GHS) classifications, highlighting their relevance for hazard assessment.
The broader research program aims to establish an integrated in vitro testing strategy suitable for regulatory decision-making in inhalation toxicology. This work contributes directly to the effort of replacing animal testing with scientifically robust, translatable, and cost-efficient alternative methods.
