VITROCELL® Powder AX12

The Organ-on-Chip Exposure Solution for Dry Powders

The VITROCELL^® Powder AX12 Exposure System is specificalley designed for direct aerosolization of smallest quantities of dry powders without the need of transfering them in suspension first. Thus, especially scarce and very expensive materials can be well used with this method, such as new drug candidates or particle samples from the environment.

Here quantities from 1 – 100 mg can be aerosolized. Aerosolization is performed directy into the cell culture exposure chamber. Aerosolization can be repeated a few times to obtain a dose-response profile.

Powder AX 12 allows for aerosolisation of small quantities of dry powders (1-100 mg) on the AX12 plate.

Working Principle

Powder sample is placed in quick-lock loading system.

Powder sample is aerosolized under high pressure to break up agglomerates for homogeneous dispersion in the expansion chamber.

Powder sample settles on the AX12 plate.

Air / Liquid Cultivation and Exposure

A: Direct and controlled exposure of test atmosphere to cells
B: Cells on ultrathin ^AXmembrane
C: Media below cells

1: Inlet well
2: Cell well
3: Outlet well
4: Fluidic chamber

AlveoliX AXBarrier-on-Chip System

The ^AXBarrier-on-Chip System allows in vitro modeling in outmost physiological conditions by replicating the essential key features of all organs in vitro, including lung, skin and eyes.

Ultrathin, porous, and elastic support for cell growth
Uni- or bidirectional mechanical stretching
Air/Liquid Interface
3D tissues (multiple co-cultures)
Tunable mechanical stimuli

The technology is a powerful tool to recreate healthy, diseased and personalized in vitro models. It provides answers to fundamental questions about biomechanics and cell biology. Furthermore, the method can be implemented in drug development to test molecule efficacy and safety, and in toxicity assessment of e. g. pollutants, chemicals and consumer goods. This will hopefully lead to more animal free product development in the future.

^AXLung-on-Chip Model

A nature-inspired concept is used to recreate the breathing motion. The ultrathin AXmembrane is deflected by applying negative pressure inside the basal chip chamber through an integrated micro-diaphragm. The combination of the breathing-induced cyclic stretch and the optimized ultrathin cell culture substrate (AXmembrane) preserves lung cells’ functionality and phenotype.

^AXActuator: The ^AXActuator is connected to the second ^AXDock placed in the incubator. It pneumatically controls the movement of the membrane inside the AX12, recreating the cyclic stretch motion.
^AXExchanger: The ^AXExchanger enables a fluid flow for initial chip filling and medium exchange by pneumatically controlling the opening and closing of the valves inside the AX12.
^AXDock: The ^AXDock connects the AX12 with the ^AXActuator and the AX Exchanger creating an air-tight sealing for the pneumatic actuation.
AX12: The AX12 is the cell culture consumable and is based on a 96-well plate format. It consists of two chips supported by a plate and includes a total of 12 independent wells.

More about VITROCELL Powder AX12

System Profile

Dry Powder Exposure
Smallest quantities of dry powder
Suitable for two AlveoliX AX12 Chips
Fully tunable and dynamic models
Connection to AX12 Actuator

AlveoliX 3D Breathing concept

A nature-inspired concept is used to recreate the breathing motion. The ultrathin ^AXmembrane (blue) is deflected by applying negative pressure inside the basal chip chamber through an integrated micro-diaphragm (grey).

Features

Easy handling with no external airflow required
Only small quantities of powder needed (1–100 mg)
Exposure time < 30 minutes
Air/Liquid Interface Exposure
Breathing Lung on chip model
Porous membrane allowing cell-cell interaction
Increased sensitivity in phsiological conditions
Versatile Organ-on-chip system (lung, skin, eyes)

Videos

AlveoliX – How can we help with our Organs-on-Chip?

Downloads

Leaflet VITROCELL® AX12 Systems Organ-on-Chip Exposure Solutions

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VITROCELL® Powder AX12

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