Advanced in vitro exposure systems.

14. Aug. 2016

Copper oxide nanoparticles: Impact on alveolar epithelial-like cells following air-liquid interface exposure

EEMGS Annual Meeting: 14 – 18 August 2016, Copenhagen, Denmark

doi:10.1007/s00204-015-1621-7

Matthias Hufnagel, Sarah Schoch, Bettina Fischer, Andrea Hartwig
Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Food Chemistry and Toxicology

This is work is part of ProCycle (No. 03XP0009), a project funded by the German Federal Ministry of Education and Research.and shows the exposition of copper oxide (CuO) nanoparticles on A549 cells at different doses in the VITROCELL® Cloud, equipped with a quartz crystal microbalance. The effects were determined using the colony formation assay (CFA) and high-throughput RT-qPCR gene expression analyses.

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29. Sep. 2015

Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure

DOI 10.1186/s12989-015-0104-6

Linda C. Stoehr1,2†, Carola Endes3†, Isabella Radauer-Preiml1, Matthew S. P. Boyles1, Eudald Casals4, Sandor Balog5, Markus Pesch2, Alke Petri-Fink3, Barbara Rothen-Rutishauser3, Martin Himly1, Martin J. D. Clift3 and Albert Duschl1*

1Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria.

2Grimm Aerosol Technik GmbH & Co. KG, Ainring, Germany.
3BioNanomaterials, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
4Institut Català de Nanotecnologia (ICN), Bellaterra, Spain.
5Soft Matter Scattering, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
* Correspondence: albert.duschl@sbg.ac.at


The use of fluorescence-based reporter cell lines can provide a useful tool in screening the pro-inflammatory response following NP exposure in the air-liquid interface. The results are shown in the IL-8 assay, qRT-PCR ans ELISA.

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27. Jun. 2015

Translocation of gold nanoparticles across the lung epithelial tissue barrier: Combining in vitro and in silico methods to substitute in vivo experiments

DOI 10.1186/s12989-015-0090-8

Gerald Bachler1,2, Sabrina Losert1,3, Yuki Umehara2, Natalie von Goetz1*, Laura Rodriguez-Lorenzo2, Alke Petri-Fink2,
Barbara Rothen-Rutishauser2 and Konrad Hungerbuehler1

1ETH Zürich, Institute for Chemical and Bioengineering, 8093 Zürich, Switzerland.
2University of Fribourg, Adolphe Merkle Institute, 1700 Fribourg, Switzerland.
3EMPA, Swiss Federal Laboratories for Material Science and Technology, 8600 Dübendorf, Switzerland.

* Correspondence: natalie.von.goetz@chem.ethz.ch

 

This replacement study for animal testings shows aerosolized nanoparticles tested on monolayer epithelial cultures in the air-liquid interface to determine their translocation kinetics across the epithelial tissue barrier.

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2. Jun. 2015

VITROCELL User Group Meeting 2015

Presentation of new VITROCELL® products and experience exchange among international scientists from Europe, Japan, Korea and USA.

The focus of the event was to present new routes for dosimetry, to give an update on VITROCELL’s participation in major European research programs related to nanoparticles as well as the presentation of new products for 2015.

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3. Apr. 2015

New VITROCELL® Cloud ALI (Air-Liquid Interface) Starter Kit

SOT and ToxExpo 2015. San Diego, USA.

Ready-to-use and economical solution for users starting to expose cell cultures at the air-liquid interface.

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20. Feb. 2015

Repeated exposure to carbon nanotube-based aerosols does not affect the functional properties of a 3D human epithelial airway model

Nanotoxicology, Early Online: 1–11 (doi: 10.3109/17435390.2014.993344)

Authors
Savvina Chortarea1, Martin J. D. Clift1, Dimitri Vanhecke1, Carola Endes1, Peter Wick2, Alke Petri-Fink1, and Barbara Rothen-Rutishauser1
1Department of BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Marly, Switzerland and
2Laboratory for Materials-Biology Interactions, EMPA, Swiss Federal Laboratories for Materials, Science and Technology, St. Gallen, Switzerland

Exposition of Carbone Nanotubes on 3D lung models in the air-liquid interface exposure system to measure oxydative stress and inflammatory response.

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23. Sep. 2014

An in vitro testing strategy towards mimicking the inhalation of high aspect ratio nanoparticles

High aspect ratio nanoparticles (HARN) were analyzed at the air/liquid interface using VITROCELL Cloud technology.

Particle and Fibre Toxicology 2014, 11:40 (doi:10.1186/s12989-014-0040-x)

Authors
Carola Endes1, Otmar Schmid2, Calum Kinnear1, Silvana Mueller3, Sandra Camarero-Espinosa3, Dimitri Vanhecke1, E. Johan Foster3, Alke Petri-Fink1,4, Barbara Rothen-Rutishauser1, Christoph Weder3 and Martin J.D. Clift.

1 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland
2 Comprehensive Pneumology Centre, Institute of Lung Biology and Disease, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
3 Polymer Chemistry and Materials, Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland
4 Department of Chemistry, University of Fribourg, Fribourg, Switzerland

 

High aspect ratio nanoparticles (HARN) were analyzed at the air-liquid interface using the VITROCELL® Cloud technology. The successful approach towards investigation of the hazard of HARN aerosols in vitro are demonstrated by measured cytotoxicity, oxidative stress and inflammatory responses. 

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20. May. 2014

VITROCELL User Group Meeting

Meeting featuring the new VITROCELL® Automated Exposure Station

International scientists from Europe, Japan, Korea and USA followed the invitation of VITROCELL® Systems to the 2014 User Group Meeting in the Europa-Park Rust, Germany.

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28. Apr. 2014

Efficient bioactive delivery of aerosolized drugs to human pulmonary epithelial cells cultured at air-liquid interface conditions

Am J Respir Cell Mol Biol

First published online 28 Apr 2014 (doi: 10.1165/rcmb.2013-0479OC)

Authors
Anke-Gabriele Lenz1, Tobias Stoeger1, Daniele Cei2, Martina Schmidmeir1, Nora Pfister1, Gerald Burgstaller1, Bernd Lentner1, Oliver Eickelberg3, Silke Meiners1 and Otmar Schmid1

1 Helmholtz Zentrum München, Institute of Lung Biology and Disease, Comprehensive Pneumology Center, Member of the German Center for Lung Research, Neuherberg/Munich, Germany
2 University of Pisa, Interdepartmental Research Center E.Piaggio”, Faculty of Engineering, Pisa, Italy
3,1 Ludwig-Maximilians-University, University Hospital, Munich, Germany

Introduction of the VITROCELL®-CLOUD (air-liquid interface cell exposure system-cloud) technology, which utilizes principles of cloud motion for fast and quantitative delivery of aerosolized liquid drugs to pulmonary cells cultured under realistic air-liquid interface conditions. These data indicate that the VITROCELL®-CLOUD is a reliable tool for aerosolized drug screening on cells.

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30. Jul. 2013

New on ICT Seoul

VITROCELL® Cloud

Exposure System for Liquid Aerosols with Cloud Dynamics.

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