Vitrocell Systems GmbH - VITROCELL Cloud

11. Mar. 2018

Elucidating the impact of nanomaterials on genomic stability using high-throughput RT-qPCR and Alkaline Unwinding

User Group Meeting 2018

Matthias Hufnagel, Prof. Dr. Andrea Hartwig

KIT - Karsruhe Institute of Technology

The poster shows the establisment of the use of a high-throughput RT-qPCR method and the quantitative detection of DNA strand breaks (Alkaline Unwinding) for cells exposed in the Air-Liquid Interface.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, microbalance, liquid aerosol, VITROCELL Cloud

29. Nov. 2017

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface

DOI: 10.1186/s12989-017-0231-3

Estelle Durantie¹, Dimitri Vanhecke¹, Laura Rodriguez-Lorenzo¹, Flavien Delhaes¹, Sandor Balog¹, Dedy Septiadi¹, Joel Bourquin¹, Alke Petri-Fink^1,2 and Barbara Rothen-Rutishauser¹

¹BioNanomaterials Group, Adolphe Merkle Institute, Université de Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.

²Chemistry Department, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

In this study, the biodistribution of aerosolized single and aggregated nanoparticles was investigated using a 3D model of the human epithelial tissue barrier. The cells were exposed at the air-liquid interface using the Vitrocell® Cloud exposure system. It consists of three main parts: a nebulizer, an aerosol chamber and a base module constituted of 12-well size inserts and connected to a controlled heating unit. Robust characterization was used to evaluate the exact delivered dose onto the cell surface and to determine the cellular uptake and translocation across the barrier.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, liquid aerosol, VITROCELL Cloud, air-liquid Interface, airborne particles

20. Aug. 2017

Development of a 3D co-culture system to assess the respiratory sensitizing potential of chemicals

10th Congress on Alternatives and Animal Use in the Life Sciences August 20–24, 2017. Seattle, Washington, USA.

A. Chary ^1,2, T. Serchi¹, J. Hennen², B. Bloemeke², J. Ezendam³, S. Cambier¹, E. Moschini¹, A.C. Gutleb¹
¹Department of Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg,
²Department of Environmental Toxicology, University of Trier, Trier, Germany,
³RIVM Centre for Environmental Protection (GZB), National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands.

The aim of this study is to adapt the 3D-co-culture system previously developed by LIST (Klein et al.1,2) to allow the in vitro study of respiratory sensitization processes and to
find markers for the assessment of the respiratory sensitizing potential of inhaled compounds and mimicking the alveolar barrier to study cell-to-cell communication and inflammatory effects of nano particles at the air-liquid-interface.

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Categories: Publications

Tags: inhalation exposure, gas exposure, VITROCELL Cloud, air-liquid Interface

6. Jul. 2017

Biokinetics of Aerosolized Liposomal Ciclosporin A in Human Lung Cells In Vitro Using an Air-Liquid Cell Interface Exposure System

DOI:10.1089/jamp.2016.1361

Schmid O^1,2, Jud C³, Umehara Y³, Mueller D⁴, Bucholski A⁴, Gruber F⁴, Denk O⁴, Egle R⁴, Petri-Fink A³, Rothen-Rutishauser B³.

¹ Comprehensive Pneumology Center (CPC), German Center for Lung Research (DZL) , Munich, Germany .
²Institute of Lung Biology and Disease, Helmholtz Zentrum München-German Research Center for Environmental Health , Neuherberg, Germany .
³ BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg, Switzerland .
⁴ PARI Pharma GmbH, Starnberg, Germany .

The lung epithelial barrier was studied in vitro. Human lung epithelial cells (alveolar A549 and bronchial 16HBE14o- epithelial cells) were exposed to an aerosolized immunosuppressive drug encapsulated in liposomes at the air-liquid interface (ALI) using a dose-controlled air-liquid interface cell exposure (ALICE) system.

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Categories: Publications

Tags: inhalation exposure, VITROCELL Cloud, air-liquid Interface

3. Jun. 2017

In Vitro Testing of Aerosolized Substances with Primary 3D Human Airway Epithelia: Application of ALICE-Cloud Technology to e-liquids (e-cigarettes)

ISAM, THE 21ST INTERNATIONAL SOCIETY OF AEROSOLS IN MEDICINE CONGRESS, 03-07 June, 2017

S. Constant¹, A. Stelzl^2,3, O. Eickelberg^2,3,4, O. Schmid^2,3
¹Epithelix, Genève, Switzerland
²Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany
³Institute of Lung Biology and Disease, Helmholtz Zentrum München, Munich/Neuherberg, Germany
⁴University Hospital of the Ludwig Maximilians University (LMU), Munich, Germany

The poster shows the ALICE-Cloud technology combined with primary ALI 3D cell cultures. The effect of nebulized e-liquids were investigated in terms of tissue integrity, celia beating frequency, mucin secretion and IL-8 secretion.

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Categories: Publications

Tags: inhalation exposure, e-cigarettes, VITROCELL Cloud, air-liquid Interface

15. May. 2017

Human Asthmatic Bronchial Cells Are More Susceptible to Subchronic Repeated Exposures of Aerosolized Carbon Nanotubes At Occupationally Relevant Doses Than Healthy Cells

DOI: 10.1021/acsnano.7b01992

Savvina Chortarea,¹ Hana Barosova,¹ Martin James David Clift,² Peter Wick,³ Alke Petri-Fink,^1,4 and Barbara Rothen-Rutishauser,¹

¹BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, CH-1700 Fribourg, Switzerland
²In Vitro Toxicology Group, Swansea University Medical School, Swansea SA2 8PP, Wales, U.K.
³Laboratory for Materials-Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science and Technology, 9014 St Gallen, Switzerland
⁴Department of Chemistry, University of Fribourg, CH-1700 Fribourg, Switzerland

The aim of this study was to realistically mimic subchronic inhalation of multiwalled carbone nanotubes in vitro, using the air−liquid interface cell exposure system for aerosol exposures on reconstituted human bronchial tissue from healthy and asthmatic donors. Cytotoxicity, cell morphology, cilia beating frequency, mucociliary clearance, gene expression analysis of oxidative stress and (pro‑)inflammatory markers as well as (pro‑)inflammatory cytokine secretion were successfully observed.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, VITROCELL Cloud, air-liquid Interface

14. Aug. 2016

Copper oxide nanoparticles: Impact on alveolar epithelial-like cells following air-liquid interface exposure

EEMGS Annual Meeting: 14 – 18 August 2016, Copenhagen, Denmark

doi:10.1007/s00204-015-1621-7

Matthias Hufnagel, Sarah Schoch, Bettina Fischer, Andrea Hartwig
Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Food Chemistry and Toxicology

This is work is part of ProCycle (No. 03XP0009), a project funded by the German Federal Ministry of Education and Research.and shows the exposition of copper oxide (CuO) nanoparticles on A549 cells at different doses in the VITROCELL® Cloud, equipped with a quartz crystal microbalance. The effects were determined using the colony formation assay (CFA) and high-throughput RT-qPCR gene expression analyses.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, microbalance, VITROCELL Cloud

29. Sep. 2015

Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure

DOI 10.1186/s12989-015-0104-6

Linda C. Stoehr^1,2†, Carola Endes^3†, Isabella Radauer-Preiml¹, Matthew S. P. Boyles¹, Eudald Casals⁴, Sandor Balog⁵, Markus Pesch², Alke Petri-Fink³, Barbara Rothen-Rutishauser³, Martin Himly¹, Martin J. D. Clift³ and Albert Duschl^1*

¹Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria.

²Grimm Aerosol Technik GmbH & Co. KG, Ainring, Germany.
³BioNanomaterials, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
⁴Institut Català de Nanotecnologia (ICN), Bellaterra, Spain.
⁵Soft Matter Scattering, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
* Correspondence: albert.duschl@sbg.ac.at

The use of fluorescence-based reporter cell lines can provide a useful tool in screening the pro-inflammatory response following NP exposure in the air-liquid interface. The results are shown in the IL-8 assay, qRT-PCR ans ELISA.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, VITROCELL Cloud, air-liquid Interface

27. Jun. 2015

Translocation of gold nanoparticles across the lung epithelial tissue barrier: Combining in vitro and in silico methods to substitute in vivo experiments

DOI 10.1186/s12989-015-0090-8

Gerald Bachler^1,², Sabrina Losert^1,3, Yuki Umehara², Natalie von Goetz^1*, Laura Rodriguez-Lorenzo², Alke Petri-Fink²,
Barbara Rothen-Rutishauser² and Konrad Hungerbuehler¹

¹ETH Zürich, Institute for Chemical and Bioengineering, 8093 Zürich, Switzerland.
²University of Fribourg, Adolphe Merkle Institute, 1700 Fribourg, Switzerland.
³EMPA, Swiss Federal Laboratories for Material Science and Technology, 8600 Dübendorf, Switzerland.

* Correspondence: natalie.von.goetz@chem.ethz.ch

This replacement study for animal testings shows aerosolized nanoparticles tested on monolayer epithelial cultures in the air-liquid interface to determine their translocation kinetics across the epithelial tissue barrier.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, VITROCELL Cloud, air-liquid Interface

2. Jun. 2015

VITROCELL User Group Meeting 2015

Presentation of new VITROCELL® products and experience exchange among international scientists from Europe, Japan, Korea and USA.

The focus of the event was to present new routes for dosimetry, to give an update on VITROCELL’s participation in major European research programs related to nanoparticles as well as the presentation of new products for 2015.

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Categories: User Group Meeting

Tags: tobacco smoke, e-cigarettes, VC 10 smoking robots, VITROCELL Cloud, exposure station, VC 1 smoking machine, User Group Meeting

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User Group Meeting 2018

10th Congress on Alternatives and Animal Use in the Life Sciences August 20–24, 2017. Seattle, Washington, USA.

ISAM, THE 21ST INTERNATIONAL SOCIETY OF AEROSOLS IN MEDICINE CONGRESS, 03-07 June, 2017

EEMGS Annual Meeting: 14 – 18 August 2016, Copenhagen, Denmark

Presentation of new VITROCELL® products and experience exchange among international scientists from Europe, Japan, Korea and USA.

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