Advanced in vitro exposure systems.

11. Mar. 2018

VITROCELL® Cloud MAX

User Group Meeting 2018

Patrick Weindl1, Dr. Otmar Schmid2,

1VITROCELL Systems GmbH, 79183 Waldkirch, Germany
2Helmholtz Center Munich, Germany

The poster shows the design for smallest nebulization volumes and high deposition efficiency.

 

Read more

Comments (0) Number of views (801)

11. Mar. 2018

VITROCELL® Cloud SEQ

User Group Meeting 2018

Patrick Weindl, Oliver Wisser

VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The VITROCELL® Cloud SEQ represents another evolution of the well-known Cloud systems. Keeping functional process parameters according to established devices like the VITROCELL® Cloud 6, 12 or 24 it is meant to enhance those devices even further. lt offers the possibility to sequentially expose rows of inserts to create dose-response curves while keeping the concentration of nebulized liquid at the same level and the cell-cultures under highly comparable conditions.


 

Read more

Comments (0) Number of views (752)

11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

Read more

Comments (0) Number of views (2938)

11. Mar. 2018

In vitro model for the prediction of respiratory sensitization of inhaled chemicals and protein allergens.

User Group Meeting 2018

Chary A.1,2, Serchi T.1, Cambier S.1, Moschini E.1, Contal S.1, Hennen J.2, Ezendam J.3, Blömeke B.2, Gutleb A.C.1
1 Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology (LIST), Luxembourg.
2 Department of Environmental Toxicology, Trier University, Germany. 3Centre for Environmental Protection,National Institute for Public Health and the Environment (RIVM), The Netherlands.

Aim
- Adapt a 3D-coculture system previously developed by us (Klein et al. 2013) allowing the study of respiratory sensitization processes by including dendritic cells, which have a crucial role in sensitization
- Find markers for the assessment of the respiratory sensitizing potential of inhaled compounds by measuring the activation of DCs as well as the release of cytokines

 

Read more

Comments (0) Number of views (963)

11. Mar. 2018

Elucidating the impact of nanomaterials on genomic stability using high-throughput RT-qPCR and Alkaline Unwinding

User Group Meeting 2018

Matthias Hufnagel, Prof. Dr. Andrea Hartwig 

KIT - Karsruhe Institute of Technology

The poster shows the establisment of the use of a high-throughput RT-qPCR method and the quantitative detection of DNA strand breaks (Alkaline Unwinding) for cells exposed in the Air-Liquid Interface.

Read more

Comments (0) Number of views (573)

29. Nov. 2017

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface

DOI: 10.1186/s12989-017-0231-3

Estelle Durantie1, Dimitri Vanhecke1, Laura Rodriguez-Lorenzo1, Flavien Delhaes1, Sandor Balog1, Dedy Septiadi1, Joel Bourquin1Alke Petri-Fink1,2 and Barbara Rothen-Rutishauser1

1BioNanomaterials Group, Adolphe Merkle Institute, Université de Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland. 

2Chemistry Department, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

 

In this study, the biodistribution of aerosolized single and aggregated nanoparticles was investigated using a 3D model of the human epithelial tissue barrier.  The cells were exposed at the air-liquid interface using the Vitrocell® Cloud exposure system. It consists of three main parts: a nebulizer, an aerosol chamber and a base module constituted of 12-well size inserts and connected to a controlled heating unit. Robust characterization was used to evaluate the exact delivered dose onto the cell surface and to determine the cellular uptake and translocation across the barrier.


Read more

Comments (0) Number of views (681)

20. Aug. 2017

Development of a 3D co-culture system to assess the respiratory sensitizing potential of chemicals

10th Congress on Alternatives and Animal Use in the Life Sciences August 20–24, 2017. Seattle, Washington, USA.

A. Chary 1,2, T. Serchi1, J. Hennen2, B. Bloemeke2, J. Ezendam3, S. Cambier1, E. Moschini1, A.C. Gutleb1
1Department of Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg,
2Department of Environmental Toxicology, University of Trier, Trier, Germany,
3RIVM Centre for Environmental Protection (GZB), National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands.

The aim of this study is to adapt the 3D-co-culture system previously developed by LIST (Klein et al.1,2) to allow the in vitro study of respiratory sensitization processes and to
find markers for the assessment of the respiratory sensitizing potential of inhaled compounds and mimicking the alveolar barrier to study cell-to-cell communication and inflammatory effects of nano particles at the air-liquid-interface.

Read more

Comments (0) Number of views (1273)

6. Jul. 2017

Biokinetics of Aerosolized Liposomal Ciclosporin A in Human Lung Cells In Vitro Using an Air-Liquid Cell Interface Exposure System

DOI:10.1089/jamp.2016.1361

Schmid O1,2, Jud C3, Umehara Y3, Mueller D4, Bucholski A4, Gruber F4, Denk O4, Egle R4, Petri-Fink A3, Rothen-Rutishauser B3.

1 Comprehensive Pneumology Center (CPC), German Center for Lung Research (DZL) , Munich, Germany .
2 Institute of Lung Biology and Disease, Helmholtz Zentrum München-German Research Center for Environmental Health , Neuherberg, Germany .
3 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg, Switzerland .
4 PARI Pharma GmbH, Starnberg, Germany .

The lung epithelial barrier was studied in vitro. Human lung epithelial cells (alveolar A549 and bronchial 16HBE14o- epithelial cells) were exposed to an aerosolized immunosuppressive drug encapsulated in liposomes at the air-liquid interface (ALI) using a dose-controlled air-liquid interface cell exposure (ALICE) system.

Read more

Comments (0) Number of views (745)

3. Jun. 2017

In Vitro Testing of Aerosolized Substances with Primary 3D Human Airway Epithelia: Application of ALICE-Cloud Technology to e-liquids (e-cigarettes)

ISAM, THE 21ST INTERNATIONAL SOCIETY OF AEROSOLS IN MEDICINE CONGRESS, 03-07 June, 2017

S. Constant1, A. Stelzl2,3, O. Eickelberg2,3,4, O. Schmid2,3
1Epithelix, Genève, Switzerland
2Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany
3Institute of Lung Biology and Disease, Helmholtz Zentrum München, Munich/Neuherberg, Germany
4University Hospital of the Ludwig Maximilians University (LMU), Munich, Germany

The poster shows the ALICE-Cloud technology combined with primary ALI 3D cell cultures. The effect of nebulized e-liquids were investigated in terms of tissue integrity, celia beating frequency, mucin secretion and IL-8 secretion.

Read more

Comments (0) Number of views (696)

15. May. 2017

Human Asthmatic Bronchial Cells Are More Susceptible to Subchronic Repeated Exposures of Aerosolized Carbon Nanotubes At Occupationally Relevant Doses Than Healthy Cells

DOI: 10.1021/acsnano.7b01992

Savvina Chortarea,1 Hana Barosova,1 Martin James David Clift,2 Peter Wick,3 Alke Petri-Fink,1,4 and Barbara Rothen-Rutishauser,1

1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, CH-1700 Fribourg, Switzerland
2In Vitro Toxicology Group, Swansea University Medical School, Swansea SA2 8PP, Wales, U.K.
3Laboratory for Materials-Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science and Technology, 9014 St Gallen, Switzerland
4Department of Chemistry, University of Fribourg, CH-1700 Fribourg, Switzerland

The aim of this study was to realistically mimic subchronic inhalation of multiwalled carbone nanotubes in vitro, using the air−liquid interface cell exposure system for aerosol exposures on reconstituted human bronchial tissue from healthy and asthmatic donors. Cytotoxicity, cell morphology, cilia beating frequency, mucociliary clearance, gene expression analysis of oxidative stress and (pro‑)inflammatory markers as well as (pro‑)inflammatory cytokine secretion were successfully observed.

 

Read more

Comments (0) Number of views (1189)
RSS
12345
Back to Top