https://dx.doi.org/10.1021/acsnano.9b06860

Autors
Barosova H¹, Maione AG², Septiadi D¹, Sharma M³, Haeni L¹, Balog S¹, O'Connell O², Jackson GR², Brown D⁴, Clippinger AJ³, Hayden P^2,5, Petri-Fink A^1,6, Stone V⁴, Rothen-Rutishauser B¹.

¹ Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
² MatTek Corporation, 200 Homer Avenue, Ashland, Massachusetts 01721, United States.
³ PETA International Science Consortium Ltd., 8 All Saints Street, London N1 9RL, U.K.
⁴ Nano-Safety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, U.K.
⁵ BioSurfaces, Inc., 200 Homer Ave, Ashland, Massachusetts 01721, United States.
⁶ Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

This study characterizes a 3D in vitro alveolar tissue model comprised entirely of primary human cells to investigate its ability to predict pulmonary fibrosis. The study demonstrated that the EpiAlveolar model recapitulates relevant lung phenotypes and functions and is stable at VITROCELL Cloud with repeated exposures over 3 weeks. 

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DOI: 10.1164/rccm.201811-2087OC

Samuel Chung ^{1 2}, Nathalie Baumlin ^{1 2}, John S Dennis ^{1 2}, Robert Moore ², Sebastian F Salathe ², Phillip L Whitney ², Juan Sabater ³, William M Abraham ³, Michael D Kim ^{1 2}, Matthias Salathe ^{1 2
1}Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas.
²Division of Pulmonary, Critical Care and Sleep Medicine, University of Miami School of Medicine, Miami, Florida; and.
³Department of Research, Mount Sinai Medical Center, Miami Beach, Florida.
 

The transient receptor potential ankyrin 1 (TRPA1) is a molecular target for vape effects due to its expression in airway epithelia and its reported gating by nicotine, reactive oxidants, and flavors, especially cinnamaldehyde. To test whether nicotine had effects independent of other e-cig vapor constituents, the Vitrocell® CLOUD exposure system was utilized to nebulize fixed nicotine doses onto the apical surface of ALI cultures. A549 cell cultures were exposed to nicotine containing e-cig vapor, produced by the VC-1 smoke exposure robot, in the air-liquid interface.

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S. Steiner¹, Sandra Ferreira¹, Moran Morelli¹, Audrey Baldi¹, Arkadiusz K. Kuczaj¹, Stefan Frentzel¹, Marco van der Toorn¹, Manuel Peitsch¹, Julia Hoeng^1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

This poster shows the investigation how aerosols are delivered within the Cloud SEQ 24 and how cell cultures respond to exposures in the system. Ibuprofen and Bortezomib was exposed to three dimensional organotypic tissue cultures of the human bronchial epithelium and biological endpoints were measured: Cytotoxicity, Interleukin 8 and Proteasome activity.

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https://doi.org/10.1016/j.tiv.2019.04.007
Yiying Wang^a, Qiangen Wu^b, Levan Muskhelishvili^c, Kelly Davis^c, Matthew Bryant^b, Xuefei Cao^a
a Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
^b Division of Biochemistry Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
^c Toxicologic Pathology Associates, Jefferson, AR 72079, United States of America

Unintentional exposure of respiratory mucosal surfaces to DHA aerosols is highly possible during sunless tanning sessions, a possibility for which it is not approved. In this study, the potential respiratory toxicity of DHA using the ALI airway tissue model is evaluated. To mimic in vivo exposure conditions, a liquid aerosol generation and exposure system was used and exposed ALI cultures to a range of non-cytotoxic doses of DHA aerosols at the air-liquid interface.

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Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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