Advanced in vitro exposure systems.

15. Apr. 2022

Aerosol–Cell Exposure System Applied to Semi-Adherent Cells for Aerosolization of Lung Surfactant and Nanoparticles Followed by High Quality RNA Extraction

https://doi.org/10.3390/nano12081362


Mélanie M. Leroux 1 , Romain Hocquel 1, Kevin Bourge 1 , Boštjan Kokot 2 , Hana Kokot 2 , Tilen Kokliˇc 2, Janez Štrancar 2, Yaobo Ding 3 , Pramod Kumar 3, Otmar Schmid 3 , Bertrand H. Rihn 1, Luc Ferrari 1 and Olivier Joubert 1
1 Institut Jean Lamour, UMR CNRS 7198, Université de Lorraine, CNRS, IJL, F-54000 Nancy, France
2 Jožef Stefan Institute, Department of Condensed Matter Physics, 1000 Ljubljana, Slovenia
3 Institute of Lung Health and Immunity, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany

The goal of this work was to refine an ALI pre-established protocol for the VITROCELL® Cloud 6 system. Therefore, after addressing each crucial step, (i.e., the use of very low concentrations of NP, the deposition of a homogeneous layer of surfactant, the specific use of semi-adherent cells, and finally the enhancement of the extraction yield of RNA to carry out transcriptomic studies), we present here for the first time a robust almost ready-to-use protocol. This one will be helpful for nanotoxicologists interested in developing such methodologies.

 

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4. Apr. 2022

Product News 04-1/2022

VITROCELL® Cloud Alpha PowerVent 

Increase of nebulizations and evacuation of residual gaseous compounds

A typical Cloud exposure allows 3 consecutive nebulizations. The PowerVent option enables an increase of the dose by adding further nebulizations. After each nebulization the humidity is removed by a short venting period before starting the new one. The venting function can be edited in the software. The PowerVent unit is delivered ready-to-use with an integrated vacuum pump.

VITROCELL® Cloud Alpha PowerVent  (PDF)

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3. Feb. 2022

Product News 02-1/2022

VITROCELL® Cloud Advanced Nebulizer

For liquid droplet sizes 9 – 12 μm with diffusor

Certain chemicals or formulations, for example nasal sprays, often have larger particle sizes than the mesh size of standard nebulizers (4.0 – 6.0 μm). The VITROCELL Cloud Advanced Nebulizer is enlarging the application areas of Cloud Exposure Systems. This special type offers the possibility to produce an aerosol containing particles in the range of 9.0 – 12.0 μm.
Our patented Cloud diffusor ensures a homogeneous distribution of even the larger droplet sizes in the Cloud Exposure Chamber. To avoid effects from larger droplet formations it has an integrated drain channel.

VITROCELL® Cloud Advanced Nebulizer  (PDF)

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9. Dec. 2021

Iota-carrageenan extracted from red algae is a potent inhibitor of SARS-CoV-2 infection in reconstituted human airway epithelia

https://doi.org/10.1016/j.bbrep.2021.101187

David Bovard b, Marco van der Toorn b, Walter K. Schlage a, Samuel Constant c, Kasper Renggli b, Manuel C. Peitsch b, Julia Hoeng b,
a Biology Consultant, Max-Baermann-Str. 21, 51429, Bergisch Gladbach, Germany
b PMI R&D, Philip Morris Products S.A, Quai Jeanrenaud 5, 2000, Neuchaˆtel, Switzerland
c Epithelix Sarl, 18 Chemin des Aulx, Plan-les-Ouates, 1228, Geneva, Switzerland

 

This study successfully demonstrates the exposure of human bronchial epithelial cultures to defined doses of nebulized Iota-carrageenan which were reproducibly generated and administered using the VITROCELL Cloud 12 system. 
The aim of this study was to further investigate the efficacy and safety of IC treatment on SARS-CoV-2 infection by using advanced in vitro models of human respiratory epithelium, the primary target and entry port of SARS-CoV-2. The experimental models were 3D cultures of reconstituted bronchial and nasal epithelia, representing the surface of the human upper respiratory tract. This apical exposure of reconstructed epithelia more closely mimics real-life exposure conditions and the absence of toxicity or any functional or structural impairment of the bronchial mucociliary epithelium demonstrates that topical treatment with nebulized IC is well tolerated at the effective concentrations. 

 

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12. Nov. 2021

Product News 11/2021

VITROCELL® S-QCM

Improved Sensor for High Precision Real-Time Dose Monitoring

The VITROCELL® sQCM 12 is the next step in the evolution of dosimetry methods in cell culture exposure systems. It simplifies the usage of conventional Quartz Crystal Microbalances suitable for VITROCELL® 12 modules while maintaining the working principle, precision and accuracy of the balances you’re used to.

VITROCELL® S-QCM  (PDF)

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12. Aug. 2021

Product News 08/2021

VITROCELL® Remote Assist Support

Let us lend you a helping hand via HoloLens

VITROCELL in vitro exposure systems are specifically manufactured according to customer specifications. Our customers in the field of research & development typically have very complex requirements for the system. This is why a VITROCELL technician is required to carry out product training as well as service & support.
We strive to provide rapid responses and problem-solving for VITROCELL users all over the world.

VITROCELL® Remote Assist Support  (PDF)

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15. Jun. 2021

VITROCELL® Cloud Alpha - A Family of Exposure Systems Suitable for Nebulized Solutions and Suspensions

Poster LIVe 2021 Kongress, 15th June 2021

Patrick Weindl, Heidi Ortolf-Wahl, Tobias Krebs

VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

Optimal Solution for Everyday Experiments  at the Air/Liquid Interface
The VITROCELL® Cloud Alpha series is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines reliable exposure of cell cultures from the respiratory tract with ease of use. The series comprises the Cloud Alpha 6, - 12, - 96 and Cloud Alpha MAX – they represent an optimal solution for everyday experiments at the Air/Liquid Interface using 6-well, 12-well, 24-well or 96-sized inserts. We have run extensive tests to ensure the Cloud Alpha series devices match our standards in terms of the established characteristics for Cloud principle exposure devices: Deposition Efficiency, Spatial Deposition and Repeatability.
 

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29. Jan. 2021

A Bioinspired in vitro Lung Model to Study Particokinetics of Nano-/Microparticles Under Cyclic Stretch and Air-Liquid Interface Conditions

DOI: 10.3389/fbioe.2021.616830 


Ali Doryab 1,2, Mehmet Berat Taskin 3, Philipp Stahlhut 3, Andreas Schröppel 1,2, Sezer Orak 1,2, Carola Voss 1,2, Arti Ahluwalia 4,5, Markus Rehberg 1,2, Anne Hilgendorff 1,2,6, Tobias Stöger 1,2, Jürgen Groll 3 and Otmar Schmid 1,2

1 Comprehensive Pneumology Center Munich, Member of the German Center for Lung Research, Munich, Germany,
2 Helmholtz Zentrum München—German Research Center for Environmental Health, Institute of Lung Biology and Disease, Munich, Germany, 
3 Department of Functional Materials in Medicine and Dentistry, Bavarian Polymer Institute, University of Würzburg, Würzburg, Germany, 
4 Research Center “E. Piaggio”, University of Pisa, Pisa, Italy, 
5 Department of Information Engineering, University of Pisa, Pisa, Italy, 
6 Center for Comprehensive Developmental Care (CDeCLMU), Dr. von Haunersches Children’s Hospital University, Hospital of the Ludwig-Maximilians University, Munich, Germany
 

We have recently introduced a novel porous and elastic membrane for in vitro cell-stretch models of the lung cultured under ALI conditions (Doryab et al., 2020). This innovative hybrid biphasic membrane, henceforth referred to as Biphasic Elastic Thin for Air-liquid culture conditions (BETA) membrane, was developed to optimize membrane characteristics for  the two phases of cell-stretch experiments under ALI conditions, namely the initial cell seeding, attachment and growth phase under submerged cell culture conditions  (phase  I)  followed  by an ALI acclimatization and cell-stretch phase at the ALI (phase II). This patented aerosol-cell exposure unit has recently been made  commercially  available  as  VITROCELL® Cloud  MAX.

 

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11. Nov. 2020

Exposure of cellulose nanocrystals on human lung cells at the air-liquid-interface

Best poster prize award at the Euro 2020 OpenTox Virtual Conference on September 21-25, 2020.

Michelle Hesler1, Annika Kittel1, Stephan Dähnhardt-Pfeiffer2, Christoph Metzger3, Christine Herrmann3, Marielle Fink4, Heiko Briesen3, Tobias Krebs4, Hagen von Briesen1, Sylvia Wagner1, Yvonne Kohl1
1 Fraunhofer Institut für Biomedizinische Technik IBMT, Sulzbach, Germany, 
2 Microscopy Services Dähnhardt GmbH, Flintbek, Germany, 
3 Lehrstuhl für Systemverfahrenstechnik, Technische Universität München, Freising, Germany, 
4 VITROCELL Systems GmbH, Fabrik Sonntag 3, Waldkirch, Germany

 

- In vitro aerosol exposure studies were performed with an air-liquid-interface (ALI)-lung model consisting of A549 (epithelial cells), EA.hy926 (endothelial cells) and THP-1 (macrophages) cells.
- Two different types of CNC extracted from α-cellulose (CNC-W) and pulp (CNC-G) by sulfuric acid hydrolysis were studied in a concentration of 100 μg/ml applied as aerosols with VITROCELL® Cloud system.
- Single and multiple exposure with and without a 24 h regeneration phase were compared.
- Endpoints of the study: Cell viability, ROS generation and DNA damage.

 

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31. Oct. 2020

Optimization of an air-liquid interface in vitro cell co-culture model to estimate the hazard of aerosol exposures

https://doi.org/10.1016/j.jaerosci.2020.105703

 

Rui-Wen He a,b, Hedwig M. Braakhuis a, Rob J. Vandebriel a, Yvonne C.M. Staal a, Eric R. Gremmer a, Paul H.B. Fokkens a, Claudia Kemp a, Jolanda Vermeulen a, Remco H.S. Westerink b, Flemming R. Cassee a,b
a National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720, BA, Bilthoven, the Netherlands
b Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80178, 3508, TD, Utrecht, the Netherlands

 

The results demonstrate the substantial differences in cellular responses including cell morphology, TEER changes, and cytotoxicity for epithelial mono-culture of 16HBE, Calu-3, H292 and BEAS-2B cells as well as the macrophage/epithelial cell co-culture models under ALI conditions. With the optimization of the co-culture procedure at the ALI, the cell co-culture models showed epithelial monolayer integrity, and increased sensitivity in inflammatory responses to LPS exposure, with the Calu-3 + MDM model giving the strongest responses.

 

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