Advanced in vitro exposure systems.

11. Nov. 2020

Exposure of cellulose nanocrystals on human lung cells at the air-liquid-interface

Best poster prize award at the Euro 2020 OpenTox Virtual Conference on September 21-25, 2020.

Michelle Hesler1, Annika Kittel1, Stephan Dähnhardt-Pfeiffer2, Christoph Metzger3, Christine Herrmann3, Marielle Fink4, Heiko Briesen3, Tobias Krebs4, Hagen von Briesen1, Sylvia Wagner1, Yvonne Kohl1
1 Fraunhofer Institut für Biomedizinische Technik IBMT, Sulzbach, Germany, 
2 Microscopy Services Dähnhardt GmbH, Flintbek, Germany, 
3 Lehrstuhl für Systemverfahrenstechnik, Technische Universität München, Freising, Germany, 
4 VITROCELL Systems GmbH, Fabrik Sonntag 3, Waldkirch, Germany

 

- In vitro aerosol exposure studies were performed with an air-liquid-interface (ALI)-lung model consisting of A549 (epithelial cells), EA.hy926 (endothelial cells) and THP-1 (macrophages) cells.
- Two different types of CNC extracted from α-cellulose (CNC-W) and pulp (CNC-G) by sulfuric acid hydrolysis were studied in a concentration of 100 μg/ml applied as aerosols with VITROCELL® Cloud system.
- Single and multiple exposure with and without a 24 h regeneration phase were compared.
- Endpoints of the study: Cell viability, ROS generation and DNA damage.

 

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29. Sep. 2020

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

https://doi.org/10.1007/s11626-020-00517-7


Xuefei Cao1, Jayme P. Coyle2, Rui Xiong1, Yiying Wang1, Robert H. Heflich1, Baiping Ren1, William M. Gwinn3, Patrick Hayden4, Liying Rojanasakul2

1 Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Rd., AR Jefferson, USA
2 Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers forDisease Control and Prevention,Morgantown,WV, USA
3 Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Durham, NC, USA
4 BioSurfaces Inc., Ashland, MA, USA


One important element for validating any new assay for making regulatory decisions is determining its performance relative to an accepted standard. Conducting in vivo inhalation toxicity studies using whole-body or nose-only exposure systems is expensive and time-consuming and typically requires a large number of animals. The goal of using alternative methods, like human in vitro ALI airway cultures, ultimately is to replace inhalation toxicity testing in animals with in vitro approaches. Transition from animal- to human-based models is ultimately expected to lead to faster and better predictive toxicity assessments and therapeutic development at lower cost.  This study shows the development and validation of alternative in vitro methods for acute toxicity testing, including acute inhalation toxicity testing.
 

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28. Sep. 2020

NanoCELL - Comprehensive characterization and human toxicological assessment of cellulose nanocrystals along their life cycle for reliable risk assessment

Yvonne Kohl1, Roland Drexel2, Christine Herrmann3, Stephan Dähnhardt-Pfeiffer4, Siegfried Fürtauer5, Michelle Hesler1, Christoph Metzger3, Marielle Fink6, Dominik Selzer7, Thorsten Lehr7, Tobias Krebs6, Sven van Lengen8, Sylvia Wagner1, Hagen von Briesen1, Felix Grimm9, Petra Weißhaupt10, Heiko Briesen3, Florian Meier2


1Fraunhofer-Institut für Biomedizinische Technik IBMT, Sulzbach, Germany; 
2Postnova Analytics GmbH, Landsberg, Germany, 
3Technische Universität München, Lehrstuhl für Systemverfahrenstechnik, Freising, Germany 
4Microscopy Services Dähnhardt GmbH, Flintbek, Germany
5Fraunhofer-Institut für Verfahrenstechnik und Verpackung IVV, Freising, Germany
6VITROCELL Systems GmbH, Waldkirch, Germany
7Universität des Saarlands, Klinische Pharmazie, Saarbrücken, Germany 
8GRÜNPERGA Papier GmbH, Grünhainichen, Germany 
9INFIANA Germany GmbH & Co. KG, Forchheim, Germany
10Umweltbundesamt, Dessau-Roßlau, Germany

 

This poster shows the hazard studies on the effect of oral and pulmonary CNC uptake. Therefore a miniaturized cloud exposure system has been developed to create an in vitro model simulating the lung and the GI tract.

 

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16. Sep. 2020

Quartz crystal microbalances (QCM) are suitable for real-time dosimetry in nanotoxicological studies using VITROCELL®Cloud cell exposure systems

https://doi.org/10.1186/s12989-020-00376-w


Yaobo Ding1,2 , Patrick Weindl1,2,3, Anke-Gabriele Lenz1,2, Paula Mayer1,2, Tobias Krebs3 and Otmar Schmid1,2
1Institute of Lung Biology and Disease, Helmholtz Zentrum München, 85764 Neuherberg, Germany
2Comprehensive Pneumology Center, Munich (CPC-M) - Member of the German Center for Lung Research (DZL), 81377 Munich, Germany
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany.

 

This study provides evidence that QCMs are suitable for real-time dosimetry in particle toxicology studies with cell cultures under air-liquid interface conditions. An experimental method for determination of LoD (lower limit of detection), accuracy and precision of QCMs using a fluorescent tracer (fluorescein salt) was presented and applied to the QCMs integrated in the VITROCELL® Cloud 6 and Cloud 12 aerosol-cell exposure systems.

 

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24. Aug. 2020

Product News 08/2020

VITROCELL® Cloud Alpha 96

96-well Air/Liquid Interface (ALI) exposure. For reliable high throughput testing of cell cultures.

The VITROCELL® Cloud Alpha 96 is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines high throughput testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12- and 24-well). It’s functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the air/liquid interface have never been easier.

VITROCELL® Cloud Alpha 96

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27. Jul. 2020

An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols

doi:10.3390/ijms21155335


Hana Barosova 1,2 , Bedia Begum Karakocak 1 , Dedy Septiadi 1 , Alke Petri-Fink 1,3, Vicki Stone 4 and Barbara Rothen-Rutishauser 1,
1 BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, 
2 Institute of Experimental Medicine of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
3 Department of Chemistry, University of Fribourg, 1700 Fribourg, Switzerland
4 Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK


The in vitro co-culture model consisting of three human cell lines were exposed at the ALI using the VITROCELL® Cloud system, equiped with QCM allowing to measure and record the deposited dose online. The nebulizer was ideal for the aerosols to suffciently mix within the entire chamber, hence resulting in uniform droplet deposition. This study shows, that this model is not limited to testing potentially hazardous nanomaterials to human cell line co-culture models.

 

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26. Jul. 2020

Comparative toxicity of ultrafine particles around a major airport in human bronchial epithelial (Calu-3) cell model at the air–liquid interface

https://doi.org/10.1016/j.tiv.2020.104950

Authors

Rui-WenHeab, Miriam E.Gerlofs-Nijlanda, JohnBoerea, PaulFokkensa, DaanLesemana, Nicole A.H.Janssena, Flemming R.Casseeab
a National Institute for Public Health and the Environment (RIVM), P.O. Box, 3720, BA, Bilthoven, the Netherlands
b Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80178, 3508, TD, Utrecht, the Netherland

 

Highlights

  • Airport and road traffic UFPs can activate inflammation in Calu-3 cells.
  • Airport UFPs exert similar toxicity compared to UFPs from road traffic emission.
  • ALI condition promotes cellular responses to particles at low exposed dose.

 

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14. Apr. 2020

Toxicity and Gene Expression Profiling of Copper- and Titanium-Based Nanoparticles Using Air–Liquid Interface Exposure

https://doi.org/10.1021/acs.chemrestox.9b00489


Matthias Hufnagel, Sarah Schoch, Johanna WallBettina, Maria Strauch, Andrea Hartwig
Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany

 

In this study an ALI exposure was combined with a high-throughput RT-qPCR approach to evaluate the toxicological potential nanoparticles in A549 cells.

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26. Mar. 2020

New lung model gives lab animals a breather

A first-of-its-kind 3D lung model could replace animals in inhalation toxicity research.

Autor

Francesca Lake

Source
Barosova H, Maione AG, Septiadi D et al. Use of EpiAlveolar lung model to predict fibrotic potential of multiwalled carbon nanotubes. ACS Nano

doi:10.1021/acsnano.9b06860 (2020);
https://pubs.acs.org/doi/10.1021/acsnano.9b06860
https://www.eurekalert.org/pub_releases/2020-03/pfte-psg032420.php

 

The model utilizes an air–liquid interface exposure device, VITROCELL Cloud, which PETA had awarded to the Heriot–Watt group in 2017 along with three other groups, in an attempt to support the development of non-animal toxicity testing methods.

 

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Categories: Publications

Tags: VITROCELL Cloud

20. Mar. 2020

Use of EpiAlveolar Lung Model to Predict Fibrotic Potential of Multiwalled Carbon Nanotubes

https://dx.doi.org/10.1021/acsnano.9b06860

Autors
Barosova H1, Maione AG2, Septiadi D1, Sharma M3, Haeni L1, Balog S1, O'Connell O2, Jackson GR2, Brown D4, Clippinger AJ3, Hayden P2,5, Petri-Fink A1,6, Stone V4, Rothen-Rutishauser B1.

1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
2 MatTek Corporation, 200 Homer Avenue, Ashland, Massachusetts 01721, United States.
3 PETA International Science Consortium Ltd., 8 All Saints Street, London N1 9RL, U.K.
4 Nano-Safety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, U.K.
5 BioSurfaces, Inc., 200 Homer Ave, Ashland, Massachusetts 01721, United States.
6 Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

 

This study characterizes a 3D in vitro alveolar tissue model comprised entirely of primary human cells to investigate its ability to predict pulmonary fibrosis. The study demonstrated that the EpiAlveolar model recapitulates relevant lung phenotypes and functions and is stable at VITROCELL Cloud with repeated exposures over 3 weeks. 

 

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