https://doi.org/10.1021/acs.chemrestox.9b00489

Matthias Hufnagel, Sarah Schoch, Johanna WallBettina, Maria Strauch, Andrea Hartwig
Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany

In this study an ALI exposure was combined with a high-throughput RT-qPCR approach to evaluate the toxicological potential nanoparticles in A549 cells.

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Autor

Francesca Lake

Source
Barosova H, Maione AG, Septiadi D et al. Use of EpiAlveolar lung model to predict fibrotic potential of multiwalled carbon nanotubes. ACS Nano

doi:10.1021/acsnano.9b06860 (2020);
https://pubs.acs.org/doi/10.1021/acsnano.9b06860
https://www.eurekalert.org/pub_releases/2020-03/pfte-psg032420.php

The model utilizes an air–liquid interface exposure device, VITROCELL Cloud, which PETA had awarded to the Heriot–Watt group in 2017 along with three other groups, in an attempt to support the development of non-animal toxicity testing methods.

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https://dx.doi.org/10.1021/acsnano.9b06860

Autors
Barosova H¹, Maione AG², Septiadi D¹, Sharma M³, Haeni L¹, Balog S¹, O'Connell O², Jackson GR², Brown D⁴, Clippinger AJ³, Hayden P^2,5, Petri-Fink A^1,6, Stone V⁴, Rothen-Rutishauser B¹.

¹ Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
² MatTek Corporation, 200 Homer Avenue, Ashland, Massachusetts 01721, United States.
³ PETA International Science Consortium Ltd., 8 All Saints Street, London N1 9RL, U.K.
⁴ Nano-Safety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, U.K.
⁵ BioSurfaces, Inc., 200 Homer Ave, Ashland, Massachusetts 01721, United States.
⁶ Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

This study characterizes a 3D in vitro alveolar tissue model comprised entirely of primary human cells to investigate its ability to predict pulmonary fibrosis. The study demonstrated that the EpiAlveolar model recapitulates relevant lung phenotypes and functions and is stable at VITROCELL Cloud with repeated exposures over 3 weeks. 

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DOI: 10.1164/rccm.201811-2087OC

Samuel Chung ^{1 2}, Nathalie Baumlin ^{1 2}, John S Dennis ^{1 2}, Robert Moore ², Sebastian F Salathe ², Phillip L Whitney ², Juan Sabater ³, William M Abraham ³, Michael D Kim ^{1 2}, Matthias Salathe ^{1 2
1}Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas.
²Division of Pulmonary, Critical Care and Sleep Medicine, University of Miami School of Medicine, Miami, Florida; and.
³Department of Research, Mount Sinai Medical Center, Miami Beach, Florida.
 

The transient receptor potential ankyrin 1 (TRPA1) is a molecular target for vape effects due to its expression in airway epithelia and its reported gating by nicotine, reactive oxidants, and flavors, especially cinnamaldehyde. To test whether nicotine had effects independent of other e-cig vapor constituents, the Vitrocell® CLOUD exposure system was utilized to nebulize fixed nicotine doses onto the apical surface of ALI cultures. A549 cell cultures were exposed to nicotine containing e-cig vapor, produced by the VC-1 smoke exposure robot, in the air-liquid interface.

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S. Steiner¹, Sandra Ferreira¹, Moran Morelli¹, Audrey Baldi¹, Arkadiusz K. Kuczaj¹, Stefan Frentzel¹, Marco van der Toorn¹, Manuel Peitsch¹, Julia Hoeng^1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

This poster shows the investigation how aerosols are delivered within the Cloud SEQ 24 and how cell cultures respond to exposures in the system. Ibuprofen and Bortezomib was exposed to three dimensional organotypic tissue cultures of the human bronchial epithelium and biological endpoints were measured: Cytotoxicity, Interleukin 8 and Proteasome activity.

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