DOI: 10.3389/fbioe.2021.616830 

Ali Doryab ^1,2, Mehmet Berat Taskin ³, Philipp Stahlhut ³, Andreas Schröppel ^1,2, Sezer Orak ^1,2, Carola Voss ^1,2, Arti Ahluwalia ^4,5, Markus Rehberg ^1,2, Anne Hilgendorff ^1,2,6, Tobias Stöger ^1,2, Jürgen Groll ³ and Otmar Schmid ^1,2

¹ Comprehensive Pneumology Center Munich, Member of the German Center for Lung Research, Munich, Germany,
² Helmholtz Zentrum München—German Research Center for Environmental Health, Institute of Lung Biology and Disease, Munich, Germany, 
³ Department of Functional Materials in Medicine and Dentistry, Bavarian Polymer Institute, University of Würzburg, Würzburg, Germany, 
⁴ Research Center “E. Piaggio”, University of Pisa, Pisa, Italy, 
⁵ Department of Information Engineering, University of Pisa, Pisa, Italy, 
⁶ Center for Comprehensive Developmental Care (CDeCLMU), Dr. von Haunersches Children’s Hospital University, Hospital of the Ludwig-Maximilians University, Munich, Germany
 

We have recently introduced a novel porous and elastic membrane for in vitro cell-stretch models of the lung cultured under ALI conditions (Doryab et al., 2020). This innovative hybrid biphasic membrane, henceforth referred to as Biphasic Elastic Thin for Air-liquid culture conditions (BETA) membrane, was developed to optimize membrane characteristics for  the two phases of cell-stretch experiments under ALI conditions, namely the initial cell seeding, attachment and growth phase under submerged cell culture conditions  (phase  I)  followed  by an ALI acclimatization and cell-stretch phase at the ALI (phase II). This patented aerosol-cell exposure unit has recently been made  commercially  available  as  VITROCELL^® Cloud  MAX.

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https://doi.org/10.1016/j.jaerosci.2020.105703

Rui-Wen He ^a,b, Hedwig M. Braakhuis ^a, Rob J. Vandebriel ^a, Yvonne C.M. Staal ^a, Eric R. Gremmer ^a, Paul H.B. Fokkens ^a, Claudia Kemp ^a, Jolanda Vermeulen ^a, Remco H.S. Westerink ^b, Flemming R. Cassee ^a,b
a National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720, BA, Bilthoven, the Netherlands
^b Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80178, 3508, TD, Utrecht, the Netherlands

The results demonstrate the substantial differences in cellular responses including cell morphology, TEER changes, and cytotoxicity for epithelial mono-culture of 16HBE, Calu-3, H292 and BEAS-2B cells as well as the macrophage/epithelial cell co-culture models under ALI conditions. With the optimization of the co-culture procedure at the ALI, the cell co-culture models showed epithelial monolayer integrity, and increased sensitivity in inflammatory responses to LPS exposure, with the Calu-3 + MDM model giving the strongest responses.

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Yvonne Kohl¹, Roland Drexel², Christine Herrmann³, Stephan Dähnhardt-Pfeiffer⁴, Siegfried Fürtauer⁵, Michelle Hesler¹, Christoph Metzger³, Marielle Fink⁶, Dominik Selzer⁷, Thorsten Lehr⁷, Tobias Krebs⁶, Sven van Lengen⁸, Sylvia Wagner¹, Hagen von Briesen¹, Felix Grimm⁹, Petra Weißhaupt¹⁰, Heiko Briesen³, Florian Meier²

¹Fraunhofer-Institut für Biomedizinische Technik IBMT, Sulzbach, Germany; 
²Postnova Analytics GmbH, Landsberg, Germany, 
³Technische Universität München, Lehrstuhl für Systemverfahrenstechnik, Freising, Germany 
⁴Microscopy Services Dähnhardt GmbH, Flintbek, Germany
⁵Fraunhofer-Institut für Verfahrenstechnik und Verpackung IVV, Freising, Germany
⁶VITROCELL Systems GmbH, Waldkirch, Germany
⁷Universität des Saarlands, Klinische Pharmazie, Saarbrücken, Germany 
⁸GRÜNPERGA Papier GmbH, Grünhainichen, Germany 
⁹INFIANA Germany GmbH & Co. KG, Forchheim, Germany
¹⁰Umweltbundesamt, Dessau-Roßlau, Germany

This poster shows the hazard studies on the effect of oral and pulmonary CNC uptake. Therefore a miniaturized cloud exposure system has been developed to create an in vitro model simulating the lung and the GI tract.

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https://doi.org/10.1016/j.pupt.2020.101939

Yue Hu ^a,b,c, Yunhua Sheng ^c,e, Xiaoli Ji ^c,e, Ping Liu ^c,e, Liming Tang ^c,e, Gang Chen ^a,Guiliang Chen ^b,d,
a School of Pharmacy, Fudan University, Shanghai, China
^b China State Institute of Pharmaceutical Industry, Shanghai, China
^c Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, China
^d Shanghai Center for Drug Evaluation and Inspection, Shanghai, China
^e NMPA Key Laboratory for Quality Analysis of Chemical Drug Preparations, Shanghai, China

In this study, the ALICE-CLOUD system was used as an easy-to-use and dosimetrically accurate ALI system for efficient delivery of the curcumin aerosol to the cells. It has been used to assess not only the respiratory effect of nanoparticles and chemicals after single or repeated exposure, but also the biokinetics of aerosolized drugs. Conventional ALI systems were mainly developed for toxicity study of inhaled substances. Compared with other systems, the ALICE-CLOUD system is not only technically simpler, easier to handle, but also has higher drug delivery efficiency and shorter exposure time. The short exposure time of the ALICE-CLOUD system was similar to the actual administration pattern of the inhaled drugs, which is suitable for preclinical development of aerosolized drugs and cellular response mechanisms for inhalation therapy.

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doi:10.3390/ijms21155335

Hana Barosova ^1,2 , Bedia Begum Karakocak ¹ , Dedy Septiadi ¹ , Alke Petri-Fink ^1,3, Vicki Stone ⁴ and Barbara Rothen-Rutishauser ¹,
¹ BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, 
² Institute of Experimental Medicine of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
³ Department of Chemistry, University of Fribourg, 1700 Fribourg, Switzerland
⁴ Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK

The in vitro co-culture model consisting of three human cell lines were exposed at the ALI using the VITROCELL® Cloud system, equiped with QCM allowing to measure and record the deposited dose online. The nebulizer was ideal for the aerosols to suffciently mix within the entire chamber, hence resulting in uniform droplet deposition. This study shows, that this model is not limited to testing potentially hazardous nanomaterials to human cell line co-culture models.

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