Increase of nebulizations and evacuation of residual gaseous compounds

A typical Cloud exposure allows 3 consecutive nebulizations. The PowerVent option enables an increase of the dose by adding further nebulizations. After each nebulization the humidity is removed by a short venting period before starting the new one. The venting function can be edited in the software. The PowerVent unit is delivered ready-to-use with an integrated vacuum pump.

VITROCELL® Cloud Alpha PowerVent  (PDF)

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https://doi.org/10.1016/j.bbrep.2021.101187

David Bovard ^b, Marco van der Toorn ^b, Walter K. Schlage ^a, Samuel Constant ^c, Kasper Renggli ^b, Manuel C. Peitsch ^b, Julia Hoeng ^b,
^a Biology Consultant, Max-Baermann-Str. 21, 51429, Bergisch Gladbach, Germany
^b PMI R&D, Philip Morris Products S.A, Quai Jeanrenaud 5, 2000, Neuchaˆtel, Switzerland
^c Epithelix Sarl, 18 Chemin des Aulx, Plan-les-Ouates, 1228, Geneva, Switzerland

This study successfully demonstrates the exposure of human bronchial epithelial cultures to defined doses of nebulized Iota-carrageenan which were reproducibly generated and administered using the VITROCELL Cloud 12 system. 
The aim of this study was to further investigate the efficacy and safety of IC treatment on SARS-CoV-2 infection by using advanced in vitro models of human respiratory epithelium, the primary target and entry port of SARS-CoV-2. The experimental models were 3D cultures of reconstituted bronchial and nasal epithelia, representing the surface of the human upper respiratory tract. This apical exposure of reconstructed epithelia more closely mimics real-life exposure conditions and the absence of toxicity or any functional or structural impairment of the bronchial mucociliary epithelium demonstrates that topical treatment with nebulized IC is well tolerated at the effective concentrations. 

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Let us lend you a helping hand via HoloLens

VITROCELL in vitro exposure systems are specifically manufactured according to customer specifications. Our customers in the field of research & development typically have very complex requirements for the system. This is why a VITROCELL technician is required to carry out product training as well as service & support.
We strive to provide rapid responses and problem-solving for VITROCELL users all over the world.

VITROCELL® Remote Assist Support  (PDF)

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DOI: 10.3389/fbioe.2021.616830 

Ali Doryab ^1,2, Mehmet Berat Taskin ³, Philipp Stahlhut ³, Andreas Schröppel ^1,2, Sezer Orak ^1,2, Carola Voss ^1,2, Arti Ahluwalia ^4,5, Markus Rehberg ^1,2, Anne Hilgendorff ^1,2,6, Tobias Stöger ^1,2, Jürgen Groll ³ and Otmar Schmid ^1,2

¹ Comprehensive Pneumology Center Munich, Member of the German Center for Lung Research, Munich, Germany,
² Helmholtz Zentrum München—German Research Center for Environmental Health, Institute of Lung Biology and Disease, Munich, Germany, 
³ Department of Functional Materials in Medicine and Dentistry, Bavarian Polymer Institute, University of Würzburg, Würzburg, Germany, 
⁴ Research Center “E. Piaggio”, University of Pisa, Pisa, Italy, 
⁵ Department of Information Engineering, University of Pisa, Pisa, Italy, 
⁶ Center for Comprehensive Developmental Care (CDeCLMU), Dr. von Haunersches Children’s Hospital University, Hospital of the Ludwig-Maximilians University, Munich, Germany
 

We have recently introduced a novel porous and elastic membrane for in vitro cell-stretch models of the lung cultured under ALI conditions (Doryab et al., 2020). This innovative hybrid biphasic membrane, henceforth referred to as Biphasic Elastic Thin for Air-liquid culture conditions (BETA) membrane, was developed to optimize membrane characteristics for  the two phases of cell-stretch experiments under ALI conditions, namely the initial cell seeding, attachment and growth phase under submerged cell culture conditions  (phase  I)  followed  by an ALI acclimatization and cell-stretch phase at the ALI (phase II). This patented aerosol-cell exposure unit has recently been made  commercially  available  as  VITROCELL^® Cloud  MAX.

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https://doi.org/10.1016/j.jaerosci.2020.105703

Rui-Wen He ^a,b, Hedwig M. Braakhuis ^a, Rob J. Vandebriel ^a, Yvonne C.M. Staal ^a, Eric R. Gremmer ^a, Paul H.B. Fokkens ^a, Claudia Kemp ^a, Jolanda Vermeulen ^a, Remco H.S. Westerink ^b, Flemming R. Cassee ^a,b
a National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720, BA, Bilthoven, the Netherlands
^b Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80178, 3508, TD, Utrecht, the Netherlands

The results demonstrate the substantial differences in cellular responses including cell morphology, TEER changes, and cytotoxicity for epithelial mono-culture of 16HBE, Calu-3, H292 and BEAS-2B cells as well as the macrophage/epithelial cell co-culture models under ALI conditions. With the optimization of the co-culture procedure at the ALI, the cell co-culture models showed epithelial monolayer integrity, and increased sensitivity in inflammatory responses to LPS exposure, with the Calu-3 + MDM model giving the strongest responses.

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