https://doi.org/10.1016/j.impact.2022.100439
Anne Bannuscher ^a, Otmar Schmid ^b,c, Barbara Drasler ^a, Alain Rohrbasser ^a, Hedwig M. Braakhuis ^d, Kirsty Meldrum ^e, Edwin P. Zwart ^d, Eric R. Gremmer ^d, Barbara Birk ^f, Manuel Rissel ^f, Robert Landsiedel ^f,g, Elisa Moschini ^h, Stephen J. Evans ^e, Pramod Kumar ^b,c, Sezer Orak ^b,c, Ali Doryab ^b,c, Johanna Samulin Erdem ⁱ, Tommaso Serchi ^h, Rob J. Vandebriel ^d, Flemming R. Cassee ^d,j, Shareen H. Doak ^e, Alke Petri-Fink ^a, Shanbeh Zienolddiny ⁱ, Martin J. D. Clift ^e, Barbara Rothen-Rutishauser ^a,
^a Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland
^b Comprehensive Pneumology Center (CPC-M), Helmholtz Zentrum München - Member of the German Center for Lung Research (DZL), Max-Lebsche-Platz 31, 81377 Munich, Germany
^c Institute of Lung Health and Immunity, Helmholtz Zentrum München – German Research Center for Environmental Health, 85764 Neuherberg, Germany
^d National Institute for Public Health and the Environment (RIVM), PO Box 1, 3720, BA, Bilthoven, the Netherlands
^e In Vitro Toxicology Group, Faculty of Medicine, Health and Life Sciences, Medical School, Institute of Life Sciences, Centre for NanoHealth, Swansea University, Singleton Campus, Wales SA2 8PP, UK
^f BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen am Rhein, Germany
^g Free University of Berlin, Pharmacy, Pharmacology and Toxicology, 14195 Berlin, Germany.
^h Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology (LIST), 41 rue du Brill, L4422 Belvaux, Grand-Duchy of Luxembourg, Luxembourg
ⁱ National Institute of Occupational Health (STAMI), N-0033 Oslo, Norway
^j Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands

Harmonization of SOP for (nano)material aerosolization using the VITROCELL® Cloud12.
Inter-laboratory comparison among seven different laboratories in Europe.

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https://doi.org/10.3390/nano12152609

Laura Maria Azzurra Camassa ¹, Elisabeth Elje ^2,3, Espen Mariussen ^2,4, Eleonora Marta Longhin ², Maria Dusinska ², Shan Zienolddiny-Narui ¹, and Elise Rundén-Pran ^2
1 National Institute of Occupational Health in Norway, 0033 Oslo, Norway
² NILU—Norwegian Institute for Air Research, 2027 Kjeller, Norway
³ Institute of Basic Medical Sciences, Department of Molecular Medicine, University of Oslo, 0372 Oslo, Norway
⁴ Norwegian Institute of Public Health, FHI, 0456 Oslo, Norway
 

This study aimed at testing the robustness and sensitivity, characterizing, and optimizing an advanced respiratory model built on human alveolar epithelial A549 cells and evaluating its response to aerosol exposure of silver NM-300K. In more detail, we compared the responses of A549 cells cultured at the ALI in the VITROCELL® 6 Cloud system in monoculture, in coculture with EA.hy926 cells, and in triculture with EA.hy926 cells and dTHP-1, and performed an interlaboratory trial across two laboratories.

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https://doi.org/10.3390/nano12081362

Mélanie M. Leroux ¹ , Romain Hocquel ¹, Kevin Bourge ¹ , Boštjan Kokot ² , Hana Kokot ² , Tilen Kokliˇc ², Janez Štrancar ², Yaobo Ding ³ , Pramod Kumar ³, Otmar Schmid ³ , Bertrand H. Rihn ¹, Luc Ferrari ¹ and Olivier Joubert ^1
1 Institut Jean Lamour, UMR CNRS 7198, Université de Lorraine, CNRS, IJL, F-54000 Nancy, France
² Jožef Stefan Institute, Department of Condensed Matter Physics, 1000 Ljubljana, Slovenia
³ Institute of Lung Health and Immunity, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany

The goal of this work was to refine an ALI pre-established protocol for the VITROCELL^® Cloud 6 system. Therefore, after addressing each crucial step, (i.e., the use of very low concentrations of NP, the deposition of a homogeneous layer of surfactant, the specific use of semi-adherent cells, and finally the enhancement of the extraction yield of RNA to carry out transcriptomic studies), we present here for the first time a robust almost ready-to-use protocol. This one will be helpful for nanotoxicologists interested in developing such methodologies.

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For liquid droplet sizes 9 – 12 μm with diffusor

Certain chemicals or formulations, for example nasal sprays, often have larger particle sizes than the mesh size of standard nebulizers (4.0 – 6.0 μm). The VITROCELL Cloud Advanced Nebulizer is enlarging the application areas of Cloud Exposure Systems. This special type offers the possibility to produce an aerosol containing particles in the range of 9.0 – 12.0 μm.
Our patented Cloud diffusor ensures a homogeneous distribution of even the larger droplet sizes in the Cloud Exposure Chamber. To avoid effects from larger droplet formations it has an integrated drain channel.

VITROCELL® Cloud Advanced Nebulizer  (PDF)

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Improved Sensor for High Precision Real-Time Dose Monitoring

The VITROCELL® sQCM 12 is the next step in the evolution of dosimetry methods in cell culture exposure systems. It simplifies the usage of conventional Quartz Crystal Microbalances suitable for VITROCELL® 12 modules while maintaining the working principle, precision and accuracy of the balances you’re used to.

VITROCELL® S-QCM  (PDF)

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