Advanced in vitro exposure systems.

16. Sep. 2020

Quartz crystal microbalances (QCM) are suitable for real-time dosimetry in nanotoxicological studies using VITROCELL®Cloud cell exposure systems

https://doi.org/10.1186/s12989-020-00376-w


Yaobo Ding1,2 , Patrick Weindl1,2,3, Anke-Gabriele Lenz1,2, Paula Mayer1,2, Tobias Krebs3 and Otmar Schmid1,2
1Institute of Lung Biology and Disease, Helmholtz Zentrum München, 85764 Neuherberg, Germany
2Comprehensive Pneumology Center, Munich (CPC-M) - Member of the German Center for Lung Research (DZL), 81377 Munich, Germany
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany.

 

This study provides evidence that QCMs are suitable for real-time dosimetry in particle toxicology studies with cell cultures under air-liquid interface conditions. An experimental method for determination of LoD (lower limit of detection), accuracy and precision of QCMs using a fluorescent tracer (fluorescein salt) was presented and applied to the QCMs integrated in the VITROCELL® Cloud 6 and Cloud 12 aerosol-cell exposure systems.

 

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24. Aug. 2020

Product News 08/2020

VITROCELL® Cloud Alpha 96

96-well Air/Liquid Interface (ALI) exposure. For reliable high throughput testing of cell cultures.

The VITROCELL® Cloud Alpha 96 is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines high throughput testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12- and 24-well). It’s functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the air/liquid interface have never been easier.

VITROCELL® Cloud Alpha 96

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27. Jul. 2020

An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols

doi:10.3390/ijms21155335


Hana Barosova 1,2 , Bedia Begum Karakocak 1 , Dedy Septiadi 1 , Alke Petri-Fink 1,3, Vicki Stone 4 and Barbara Rothen-Rutishauser 1,
1 BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, 
2 Institute of Experimental Medicine of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
3 Department of Chemistry, University of Fribourg, 1700 Fribourg, Switzerland
4 Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK


The in vitro co-culture model consisting of three human cell lines were exposed at the ALI using the VITROCELL® Cloud system, equiped with QCM allowing to measure and record the deposited dose online. The nebulizer was ideal for the aerosols to suffciently mix within the entire chamber, hence resulting in uniform droplet deposition. This study shows, that this model is not limited to testing potentially hazardous nanomaterials to human cell line co-culture models.

 

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26. Jul. 2020

Comparative toxicity of ultrafine particles around a major airport in human bronchial epithelial (Calu-3) cell model at the air–liquid interface

https://doi.org/10.1016/j.tiv.2020.104950

Authors

Rui-WenHeab, Miriam E.Gerlofs-Nijlanda, JohnBoerea, PaulFokkensa, DaanLesemana, Nicole A.H.Janssena, Flemming R.Casseeab
a National Institute for Public Health and the Environment (RIVM), P.O. Box, 3720, BA, Bilthoven, the Netherlands
b Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80178, 3508, TD, Utrecht, the Netherland

 

Highlights

  • Airport and road traffic UFPs can activate inflammation in Calu-3 cells.
  • Airport UFPs exert similar toxicity compared to UFPs from road traffic emission.
  • ALI condition promotes cellular responses to particles at low exposed dose.

 

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14. Apr. 2020

Toxicity and Gene Expression Profiling of Copper- and Titanium-Based Nanoparticles Using Air–Liquid Interface Exposure

https://doi.org/10.1021/acs.chemrestox.9b00489


Matthias Hufnagel, Sarah Schoch, Johanna WallBettina, Maria Strauch, Andrea Hartwig
Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany

 

In this study an ALI exposure was combined with a high-throughput RT-qPCR approach to evaluate the toxicological potential nanoparticles in A549 cells.

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27. Mar. 2020

Reliable in vitro Exposure Systems for Screening of Antiviral Lead Compounds against Coronavirus (COVID-19 / SARS-CoV-2)

VITROCELL Application Note for Highly efficient and realistic application of aerosolized drugs to ­human cells of the respiratory tract under physiologic conditions

In inhalation therapy, drugs are deposited as aerosols on cells of the respiratory tract from the nasal or lung region. Physiologically realistic in vitro cell culture models of the pulmonary epithelium and the air-blood barrier as well as from the nasal region are commercially available. Recently, these models have been refined to mimic  SARS-CoV-2 infections.

VITROCELL Application Note.

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26. Mar. 2020

New lung model gives lab animals a breather

A first-of-its-kind 3D lung model could replace animals in inhalation toxicity research.

Autor

Francesca Lake

Source
Barosova H, Maione AG, Septiadi D et al. Use of EpiAlveolar lung model to predict fibrotic potential of multiwalled carbon nanotubes. ACS Nano

doi:10.1021/acsnano.9b06860 (2020);
https://pubs.acs.org/doi/10.1021/acsnano.9b06860
https://www.eurekalert.org/pub_releases/2020-03/pfte-psg032420.php

 

The model utilizes an air–liquid interface exposure device, VITROCELL Cloud, which PETA had awarded to the Heriot–Watt group in 2017 along with three other groups, in an attempt to support the development of non-animal toxicity testing methods.

 

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Categories: Publications

Tags: VITROCELL Cloud

26. Mar. 2020

Screening antiviraler pharmazeutischer Leitwirkstoffe gegen das Coronavirus (COVID-19 / SARS-CoV-2)

VITROCELL in vitro Expositionssysteme für hocheffiziente und realistische Applikation aerosolisierter Medikamente auf menschliche Zellen des Atemtraktes unter physiologischen Bedingungen.

In der Inhalationstherapie werden pharmazeutische Präparate als Aerosole auf Zellen des Atemtraktes im Nasen- oder Lungenbereich appliziert. Zur präklinischen Entwicklung neuer Arzneimittel werden dafür oft kommerziell erhältliche, physiologisch relevante in vitro Zellkulturmodelle des Nasen-, Rachen- oder Lungenepithels verwendet. In jüngster Zeit wurden diese Modelle verfeinert, um SARS-CoV-2 Infektionen nachzuahmen.

VITROCELL Anwendungshinweis.

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20. Mar. 2020

Use of EpiAlveolar Lung Model to Predict Fibrotic Potential of Multiwalled Carbon Nanotubes

https://dx.doi.org/10.1021/acsnano.9b06860

Autors
Barosova H1, Maione AG2, Septiadi D1, Sharma M3, Haeni L1, Balog S1, O'Connell O2, Jackson GR2, Brown D4, Clippinger AJ3, Hayden P2,5, Petri-Fink A1,6, Stone V4, Rothen-Rutishauser B1.

1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
2 MatTek Corporation, 200 Homer Avenue, Ashland, Massachusetts 01721, United States.
3 PETA International Science Consortium Ltd., 8 All Saints Street, London N1 9RL, U.K.
4 Nano-Safety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, U.K.
5 BioSurfaces, Inc., 200 Homer Ave, Ashland, Massachusetts 01721, United States.
6 Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

 

This study characterizes a 3D in vitro alveolar tissue model comprised entirely of primary human cells to investigate its ability to predict pulmonary fibrosis. The study demonstrated that the EpiAlveolar model recapitulates relevant lung phenotypes and functions and is stable at VITROCELL Cloud with repeated exposures over 3 weeks. 

 

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19. Feb. 2020

Differences in cytotoxicity of lung epithelial cells exposed to titanium dioxide nanofibers and nanoparticles: Comparison of air-liquid interface and submerged cellcultures

doi: 10.1016/j.tiv.2020.104798

Autors

Medina-Reyes EI1, Delgado-Buenrostro NL2, Leseman DL3, Déciga-Alcaraz A2, He R4, Gremmer ER3, Fokkens PHB3, Flores-Flores JO5, Cassee FR4, Chirino YI2.
1 Laboratorio de Carcinogénesis y Toxicología, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Estado de México, Mexico. Electronic address: medinaingrid0@gmail.com.
2 Laboratorio de Carcinogénesis y Toxicología, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Estado de México, Mexico.
3 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
4 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands; Institute of Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands.
5 Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, CP 04510 Ciudad de México, Mexico

 

The Air-liquid interface (ALI) model has emerged as a closer physiological system that mimics exposure in gaseous and liquid phases. This Study shows that the exposure to TiO2 nanofibers and nanoparticles displays similar toxicity both the ALI and submerged cell cultures, using lung epithelial A549 cells. Additionally, they detected for the first time that TiO2 nanofibers were located into the nucleus.

 

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