Michelle Hesler¹, Annika Kittel¹, Stephan Dähnhardt-Pfeiffer², Christoph Metzger³, Christine Herrmann³, Marielle Fink⁴, Heiko Briesen³, Tobias Krebs⁴, Hagen von Briesen¹, Sylvia Wagner¹, Yvonne Kohl^1
1 Fraunhofer Institut für Biomedizinische Technik IBMT, Sulzbach, Germany, 
² Microscopy Services Dähnhardt GmbH, Flintbek, Germany, 
³ Lehrstuhl für Systemverfahrenstechnik, Technische Universität München, Freising, Germany, 
⁴ VITROCELL Systems GmbH, Fabrik Sonntag 3, Waldkirch, Germany

- In vitro aerosol exposure studies were performed with an air-liquid-interface (ALI)-lung model consisting of A549 (epithelial cells), EA.hy926 (endothelial cells) and THP-1 (macrophages) cells.
- Two different types of CNC extracted from α-cellulose (CNC-W) and pulp (CNC-G) by sulfuric acid hydrolysis were studied in a concentration of 100 μg/ml applied as aerosols with VITROCELL® Cloud system.
- Single and multiple exposure with and without a 24 h regeneration phase were compared.
- Endpoints of the study: Cell viability, ROS generation and DNA damage.

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https://doi.org/10.1186/s12989-020-00376-w

Yaobo Ding^1,2 , Patrick Weindl1,2,3, Anke-Gabriele Lenz^1,2, Paula Mayer^1,2, Tobias Krebs³ and Otmar Schmid^1,2
1Institute of Lung Biology and Disease, Helmholtz Zentrum München, 85764 Neuherberg, Germany
²Comprehensive Pneumology Center, Munich (CPC-M) - Member of the German Center for Lung Research (DZL), 81377 Munich, Germany
³VITROCELL Systems GmbH, 79183 Waldkirch, Germany.

This study provides evidence that QCMs are suitable for real-time dosimetry in particle toxicology studies with cell cultures under air-liquid interface conditions. An experimental method for determination of LoD (lower limit of detection), accuracy and precision of QCMs using a fluorescent tracer (fluorescein salt) was presented and applied to the QCMs integrated in the VITROCELL® Cloud 6 and Cloud 12 aerosol-cell exposure systems.

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doi:10.3390/ijms21155335

Hana Barosova ^1,2 , Bedia Begum Karakocak ¹ , Dedy Septiadi ¹ , Alke Petri-Fink ^1,3, Vicki Stone ⁴ and Barbara Rothen-Rutishauser ¹,
¹ BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, 
² Institute of Experimental Medicine of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
³ Department of Chemistry, University of Fribourg, 1700 Fribourg, Switzerland
⁴ Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK

The in vitro co-culture model consisting of three human cell lines were exposed at the ALI using the VITROCELL® Cloud system, equiped with QCM allowing to measure and record the deposited dose online. The nebulizer was ideal for the aerosols to suffciently mix within the entire chamber, hence resulting in uniform droplet deposition. This study shows, that this model is not limited to testing potentially hazardous nanomaterials to human cell line co-culture models.

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https://doi.org/10.1021/acs.chemrestox.9b00489

Matthias Hufnagel, Sarah Schoch, Johanna WallBettina, Maria Strauch, Andrea Hartwig
Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany

In this study an ALI exposure was combined with a high-throughput RT-qPCR approach to evaluate the toxicological potential nanoparticles in A549 cells.

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Autor

Francesca Lake

Source
Barosova H, Maione AG, Septiadi D et al. Use of EpiAlveolar lung model to predict fibrotic potential of multiwalled carbon nanotubes. ACS Nano

doi:10.1021/acsnano.9b06860 (2020);
https://pubs.acs.org/doi/10.1021/acsnano.9b06860
https://www.eurekalert.org/pub_releases/2020-03/pfte-psg032420.php

The model utilizes an air–liquid interface exposure device, VITROCELL Cloud, which PETA had awarded to the Heriot–Watt group in 2017 along with three other groups, in an attempt to support the development of non-animal toxicity testing methods.

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