S. Steiner¹, Sandra Ferreira¹, Moran Morelli¹, Audrey Baldi¹, Arkadiusz K. Kuczaj¹, Stefan Frentzel¹, Marco van der Toorn¹, Manuel Peitsch¹, Julia Hoeng^1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

This poster shows the investigation how aerosols are delivered within the Cloud SEQ 24 and how cell cultures respond to exposures in the system. Ibuprofen and Bortezomib was exposed to three dimensional organotypic tissue cultures of the human bronchial epithelium and biological endpoints were measured: Cytotoxicity, Interleukin 8 and Proteasome activity.

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https://doi.org/10.1016/j.tiv.2019.04.007
Yiying Wang^a, Qiangen Wu^b, Levan Muskhelishvili^c, Kelly Davis^c, Matthew Bryant^b, Xuefei Cao^a
a Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
^b Division of Biochemistry Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
^c Toxicologic Pathology Associates, Jefferson, AR 72079, United States of America

Unintentional exposure of respiratory mucosal surfaces to DHA aerosols is highly possible during sunless tanning sessions, a possibility for which it is not approved. In this study, the potential respiratory toxicity of DHA using the ALI airway tissue model is evaluated. To mimic in vivo exposure conditions, a liquid aerosol generation and exposure system was used and exposed ALI cultures to a range of non-cytotoxic doses of DHA aerosols at the air-liquid interface.

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DOI:10.14573/altex.1901241

Aline Chary^1,2, Tommaso Serchi¹, Elisa Moschini¹, Jennifer Hennen², Sébastien Cambier¹, Janine Ezendam³, Brunhilde Blömeke² and Arno C. Gutleb^1
1Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Luxembourg; 
²Department of Environmental Toxicology, University Trier, Germany; 
³Centre for Health Protection, National Institute for Public Health and the Environment, The Netherlands

The Vitrocell® Cloud-6 system was used for the exposure of a coculture in vitro model to test compounds and vehicle controls. The 3D in vitro model was cultured at the air liquid interface consists of A549, EA.hy926, PMA-differentiated THP-1 and non-differentiated THP-1 cells, that were exposed to nebulized chemical respiratory sensitizers. The results are shown in flow cytometry measurements, cytokine release and gene expression.

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DOI: 10.2217/nnm-2017-0358

Savvina Chortarea^1,2, Kleanthis Fytianos^1,3,4, Laura Rodriguez-Lorenzo¹, Alke Petri-Fink^1,5 & Barbara Rothen-Rutishauser^1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
²Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
³Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
⁴Department of Clinical Research, University of Bern, Bern, Switzerland
⁵Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

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Patrick Weindl¹, Dr. Otmar Schmid²,

¹VITROCELL Systems GmbH, 79183 Waldkirch, Germany
²Helmholtz Center Munich, Germany

The poster shows the design for smallest nebulization volumes and high deposition efficiency.

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