https://doi.org/10.3390/nano11010065

Silvia Diabaté ¹, Lucie Armand ², Sivakumar Murugadoss ¹ , Marco Dilger ¹ , Susanne Fritsch-Decker ¹, Christoph Schlager ³, David Béal ², Marie-Edith Arnal ², Mathilde Biola-Clier ², Selina Ambrose ⁴, Sonja Mülhopt ³, Hanns-Rudolf Paur ³, Iseult Lynch ⁵ , Eugenia Valsami-Jones ⁵ , Marie Carriere ², and Carsten Weiss ¹

¹ Karlsruhe Institute of Technology, Institute of Biological and Chemical Systems–Biological Information Processing, 76344 Eggenstein-Leopoldshafen, Germany;
² CEA, CNRS, IRIG, SyMMES, University Grenoble Alpes, 38054 Grenoble, France;
³ Karlsruhe Institute of Technology, Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, Germany; 
⁴ Promethean Particles Ltd., Nottingham NG7 3EF, UK;
⁵ School of Geography Earth & Environmental Sciences (GEES), University of Birmingham (UoB), Edgbaston, Birmingham B15 2TT, UK;

KIT, together with VITROCELL SYSTEMS, set up a first Automated Exposure Station, which has been used for the assessment of nanoscale particle emissions from combustion sources such as ship diesel and wood burners. The system was further developed and offers a compact solution for toxicity testing of nanoparticle (NP) aerosols including sample conditioning, reproducible deposition, integrated dose determination by a quartz crystal microbalance (QCM), flow control, automated processes and data acquisition. The device was also tested with partner laboratories with the aim of potentially standardizing and achieving regulatory acceptance of the method.

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DOI: 10.1002/jat.4113

Clémence Méausoone¹, Yann Landkocz¹, Fabrice Cazier², Marianne Seigneur¹ Dominique Courcot¹, Sylvain Billet^1
1Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkirk, France
²Centre Commun de Mesures, Université du Littoral Côte d'Opale, Dunkirk, France

Benzene and toluene are solvents largely used in industries and emitted into the atmosphere, despite major and direct impacts on human health. This study shows the feasibility of observing mechanisms of toxic action during repeated exposure at ALI to doses close to human exposure. The second strength of this study was the measure of XME induction level with the initiation of several xenobiotic metabolism pathways over time. BEAS-2B cells were exposed to benzene, toluene, m-xylene, or mesitylene gaseous stream diluted in air using the Vitrocell ALI system.

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https ://doi.org/10.1186/s1293 1-020-01539-1

Rakeysha Pinkston^1,2, Hasan Zaman², Ekhtear Hossain², Arthur L. Penn² and Alexandra Noël^2
1 Department of Environmental Toxicology, College of Sciences and Engineering, Southern University and A&M College, Baton Rouge, LA 70813, USA. 
² Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA.

There are thousands of flavors and flavoring combinations of e-liquids on the market with the potential to produce harmful effects when aerosolized through an ENDS device. While more research is needed regarding the potential toxicity associated with inhaling flavoring additives in combination with nicotine salt for future regulation of ENDS products, the present study provides laboratory-based evidence that should be considered regarding regulation of nicotine salt-based products.

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Sonja Mülhopt¹, Christoph Schlager², Markus Berger², Sivakumar Murugadoss³, Peter H. Hoet³, Tobias Krebs², Hanns-Rudolf paur¹ & Dieter Stapf^1
1Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, Eggenstein-Leopoldshafen, 76344, Germany. 
²Vitrocell Systems GmbH, Waldkirch, 79183, Germany. 
³KU Leuven, Environment and Health, Leuven, 3000, Belgium.

The surface dose and the spatial distribution on the membrane delivers important data for  measuring dose-response relationships in toxicity studies.  Image evaluation of transmission electron  microscopy (TEM) samples is a highly sensitive method for determination of deposition. This paper reports  the development and characterization of a novel holder for film coated TEM copper grids, which allows for  sampling under identical geometric and ambient conditions as in a cell culture chamber. 

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DOI: 10.1016/j.chemosphere.2019.125126 

Autors

Benjamin M King ¹ , Nathan J Janechek ¹ , Nathan Bryngelson ¹ , Andrea Adamcakova-Dodd ² , Traci Lersch ³ , Kristin Bunker ³ , Gary Casuccio ³ , Peter S Thorne ² , Charles O Stanier ⁴ , Jennifer Fiegel ⁵ 

¹Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
²Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

A549 Lung cells were exposed to the secondary organosilicon aerosols using the Vitrocell 6 air-liquid interface system.

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