Sonja Mülhopt¹, Christoph Schlager², Markus Berger², Sivakumar Murugadoss³, Peter H. Hoet³, Tobias Krebs², Hanns-Rudolf paur¹ & Dieter Stapf^1
1Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, Eggenstein-Leopoldshafen, 76344, Germany. 
²Vitrocell Systems GmbH, Waldkirch, 79183, Germany. 
³KU Leuven, Environment and Health, Leuven, 3000, Belgium.

The surface dose and the spatial distribution on the membrane delivers important data for  measuring dose-response relationships in toxicity studies.  Image evaluation of transmission electron  microscopy (TEM) samples is a highly sensitive method for determination of deposition. This paper reports  the development and characterization of a novel holder for film coated TEM copper grids, which allows for  sampling under identical geometric and ambient conditions as in a cell culture chamber. 

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DOI: 10.1016/j.chemosphere.2019.125126 

Autors

Benjamin M King ¹ , Nathan J Janechek ¹ , Nathan Bryngelson ¹ , Andrea Adamcakova-Dodd ² , Traci Lersch ³ , Kristin Bunker ³ , Gary Casuccio ³ , Peter S Thorne ² , Charles O Stanier ⁴ , Jennifer Fiegel ⁵ 

¹Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
²Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

A549 Lung cells were exposed to the secondary organosilicon aerosols using the Vitrocell 6 air-liquid interface system.

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https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keyser^a, Robert Leverette^a, Michael Hollings^b, Adam Seymour^b, Lesley Reeve^b, Wanda Fields^a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
^b Covance Laboratories Ltd., North Yorkshire, UK

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

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DOI: 10.1016/j.chemosphere.2019.125126

Autors

King BM¹, Janechek NJ¹, Bryngelson N¹, Adamcakova-Dodd A², Lersch T³, Bunker K³, Casuccio G³, Thorne PS², Stanier CO⁴, Fiegel J⁵.

¹ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
² Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³ RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

Highlights
• Oxidative flow reactor used to study effects of secondary aerosols on lung cells.
• Nanoparticulate aerosols generated from OH oxidation of D5, a cyclic siloxane.
• Acute exposures to 54–116 ng/cm² achieved using the air-liquid interface (ALI) system.
• Cytotoxic and proinflammatory effects marginal or absent at these doses.

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DOI: 10.1089/aivt.2017.0042

David Thorne, Emma Bishop, Linsey Haswell, and Marianna Gaca
British American Tobacco Group R&D, Southampton, United Kingdom

This case study has demonstrated that dosimetry techniques using particulate markers can allow in vitro biological data generated on five independent aerosol exposure system studies, under contrasting experimental designs and with different cell types, to be directly compared.

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