Sonja Mülhopt¹, Christoph Schlager², Markus Berger², Sivakumar Murugadoss³, Peter H. Hoet³, Tobias Krebs², Hanns-Rudolf paur¹ & Dieter Stapf^1
1Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, Eggenstein-Leopoldshafen, 76344, Germany. 
²Vitrocell Systems GmbH, Waldkirch, 79183, Germany. 
³KU Leuven, Environment and Health, Leuven, 3000, Belgium.

The surface dose and the spatial distribution on the membrane delivers important data for  measuring dose-response relationships in toxicity studies.  Image evaluation of transmission electron  microscopy (TEM) samples is a highly sensitive method for determination of deposition. This paper reports  the development and characterization of a novel holder for film coated TEM copper grids, which allows for  sampling under identical geometric and ambient conditions as in a cell culture chamber. 

Read more

DOI: 10.1016/j.chemosphere.2019.125126 

Autors

Benjamin M King ¹ , Nathan J Janechek ¹ , Nathan Bryngelson ¹ , Andrea Adamcakova-Dodd ² , Traci Lersch ³ , Kristin Bunker ³ , Gary Casuccio ³ , Peter S Thorne ² , Charles O Stanier ⁴ , Jennifer Fiegel ⁵ 

¹Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
²Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

A549 Lung cells were exposed to the secondary organosilicon aerosols using the Vitrocell 6 air-liquid interface system.

Read more

Robert Leverette¹, Brian Keyser¹, Michael Hollings² and Adam Seymour^2
1RAI Services Company, Winston−Salem, NC 27101 USA
²Covance Laboratories Ltd., Harrogate, North Yorkshire HG3 1PY, UK

• Freshly generated whole smoke / aerosol from three different tobacco product types (3R4F, THP and ENDS) was consistently delivered within the AMES 48 and 6/48 exposure modules.
• Biological endpoints: 3R4F whole smoke induced increased revertant counts (Ames) and cytotoxicity (NRU) in the AMES 48 and 6/48 modules, respectively.
• Revertant counts (Ames) and cytotoxicity (IC50) values were comparable to those from the standard exposure modules when run under similar exposure conditions.
• Overall, the AMES 48 and 6/48 modules are deemed “Fit for Use" for the in vitrO evaluation of different tobacco product types (combustible, THP, ENDS).
 

Read more

https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keyser^a, Robert Leverette^a, Michael Hollings^b, Adam Seymour^b, Lesley Reeve^b, Wanda Fields^a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
^b Covance Laboratories Ltd., North Yorkshire, UK

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

Read more

DOI: 10.1016/j.chemosphere.2019.125126

Autors

King BM¹, Janechek NJ¹, Bryngelson N¹, Adamcakova-Dodd A², Lersch T³, Bunker K³, Casuccio G³, Thorne PS², Stanier CO⁴, Fiegel J⁵.

¹ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
² Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³ RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

Highlights
• Oxidative flow reactor used to study effects of secondary aerosols on lung cells.
• Nanoparticulate aerosols generated from OH oxidation of D5, a cyclic siloxane.
• Acute exposures to 54–116 ng/cm² achieved using the air-liquid interface (ALI) system.
• Cytotoxic and proinflammatory effects marginal or absent at these doses.

Read more