Advanced in vitro exposure systems.

18. Jan. 2023

Comparing the Toxicological Responses of Pulmonary Air–Liquid Interface Models upon Exposure to Differentially Treated Carbon Fibers

https://doi.org/10.3390/ijms24031927

Alexandra Friesen 1, Susanne Fritsch-Decker 2, Sonja Mülhopt 3 , Caroline Quarz 1, Jonathan Mahl 3, Werner Baumann 3, Manuela Hauser 3, Manuela Wexler 3, Christoph Schlager 4, Bastian Gutmann 4, Tobias Krebs 4, Ann-Kathrin Goßmann 5 , Frederik Weis 5 , Matthias Hufnagel 1, Dieter Stapf 3 , Andrea Hartwig 1, and Carsten Weiss 2,
1 Karlsruhe Institute of Technology (KIT), Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, 76131 Karlsruhe, Germany
2 Karlsruhe Institute of Technology (KIT), Institute of Biological and Chemical Systems, Biological Information Processing, 76344 Eggenstein-Leopoldshafen, Germany
3 Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, Germany
4 Vitrocell Systems GmbH, 79183 Waldkirch, Germany
5 Palas GmbH, 76229 Karlsruhe, Germany

 

In this study, we assessed the toxicological responses to mechanically (mCF) and thermo-mechanically treated PAN-based carbon fibers (tmCF), applied to BEAS-2B mono-, BEAS-2B/dTHP-1 co-, and BEAS-2B/dTHP-1/CCD-33Lu triple-cultures at an air–liquid interface. The results emphasize the need to further characterize the hazard of CF and the importance of determining exposure levels at workplaces as well as safety measures during the use of CF-containing materials. Moreover, the development and application of more complex in vitro models of the human lung help to establish new approach methodologies in the general field of particle and fiber toxicology and contribute to a reduction in in vivo investigations in the context of the 3R initiative.

 

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3. Nov. 2022

Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module

https://doi.org/10.1016/j.toxrep.2022.11.001

Brian M. Keyser1, Robert Leverette1, Michael Hollings2, Adam Seymour2, Randy A. Weidman3, Carlton J. Bequette3, Kristen Jordan1
1 RAI Services Company; Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
2 Labcorp Early Development Laboratories Ltd., Harrogate, North Yorkshire, UK
3 RJ Reynolds Tobacco Company; 950 Reynolds Blvd., Winston-Salem, NC 27106, USA

 

Highlights

  • Characterization of two 48 well exposure modules with three different product types
  • Comparison of these exposure modules using four different dosimetry techniques
  • Ability to differentiate the aerosol deliveries between different dilution airflows

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13. Oct. 2022

Toxicological impact of organic ultrafine particles (UFPs) in human bronchial epithelial BEAS-2B cells at air-liquid interface

https://doi.org/10.1016/j.tiv.2021.105258

A. T. Juarez Facio1, J. Yon2, C. Corbière1, T. Rogez-Florent1, C. Castilla3, H. Lavanant3, M. Mignot3, C. Devouge-Boyer3, C. Logie1, L. Chevalier 4, J.-M. Vaugeois1, C. Monteil 1
1 Normandie Univ, UNIROUEN, UNICAEN ABTE, 76000 Rouen, France
2 Normandie Univ, UNIROUEN, INSA Rouen, CNRS, CORIA, 76000 Rouen, France.
3 Normandie Univ, INSA Rouen, UMR 6014 CNRS, COBRA, 76801, Saint Etienne Du Rouvray, France
4 Normandie Univ, UNIROUEN, INSA Rouen, CNRS, GPM-UMR6634, 76000 Rouen, France

 

Highlights

  • BEAS-2B cells were successfully exposed at ALI to primary UFPs emissions
  • PAHs-rich UFPs induced an acute cellular toxicity at low exposed dose
  • PAHs-rich UFPs activated oxidative stress and xenobiotic metabolism
  • This methodological approach offers new perspectives to evaluate different kinds of UFPs

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12. Aug. 2021

Product News 08/2021

VITROCELL® Remote Assist Support

Let us lend you a helping hand via HoloLens

VITROCELL in vitro exposure systems are specifically manufactured according to customer specifications. Our customers in the field of research & development typically have very complex requirements for the system. This is why a VITROCELL technician is required to carry out product training as well as service & support.
We strive to provide rapid responses and problem-solving for VITROCELL users all over the world.

VITROCELL® Remote Assist Support  (PDF)

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27. Jun. 2021

Impact of Nanocomposite Combustion Aerosols on A549 Cells and a 3D Airway Model

https://doi.org/10.3390/nano11071685

Matthias Hufnagel 1, Nadine May 2, Johanna Wall 1, Nadja Wingert 3, Manuel Garcia-Käufer 3, Ali Arif 3, Christof Hübner 4, Markus Berger 5, Sonja Mülhopt 2, Werner Baumann 2, Frederik Weis 6, Tobias Krebs 5, Wolfgang Becker 4, Richard Gminski 3, Dieter Stapf 2, and Andrea Hartwig 1,


1 Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany;
2 Institute for Technical Chemistry, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany; 
3 Institute for Infection Prevention and Hospital Epidemiology, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, 79110 Freiburg, Germany;
4 Fraunhofer Institute of Chemical Technology, 76327 Pfinztal, Germany; 
5 Vitrocell® Systems GmbH, 79183 Waldkirch, Germany; 
6 Palas GmbH, 76229 Karlsruhe, Germany; 

 

This study was the first to investigate the toxicological effects of well characterized aerosols released during combustion of thermoplastic nanocomposites using an air–liquid interface exposure system. Even though studies on the toxicological potential of combustion-generated particulate matter as well as VOCs have been published, none of them was designed to investigate the effect of the native aerosol using appropriate realistic lung cell culture models. In the current study we investigated the combustion behavior of PE-based nanocomposites on a lab-scale burner. As nanoscaled fillers TiO2 NP, CuO NP, as well as CNT were chosen for this study, with TiO2 NP representing a commonly used insoluble and inert nanomaterial, CuO NP as a known in vitro cyto- as well as genotoxic nanomaterial, and CNT as a fiber-shaped nanomaterial.

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21. Jun. 2021

In vitro hazard characterization of simulated aircraft cabin bleed-air contamination in lung models using an air-liquid interface (ALI) exposure system

https://doi.org/10.1016/j.envint.2021.106718

Rui-Wen He a,b, Marc M.G. Houtzager c, W.P. Jongeneel a, Remco H.S. Westerink b, Flemming R. Cassee a,b

a National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, the Netherlands
b Institute for Risk Assessment Sciences (IRAS), Toxicology Division, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80177, 3508 TD Utrecht, the Netherlands
c The Netherlands Organisation for Applied Scientific Research, TNO, P.O. Box 80015, 3508 TA Utrecht, the Netherlands

 

This unique experimental “Mini-BACS + AES” setup is able to provide steady conditions to perform in vitro exposure under ALI conditions to aircraft engine oil and hydraulic fluid fumes, generated at respectively 350 ◦C and 200 ◦C. Exposure of the Calu-3 monoculture and Calu-3 + MDM co-culture lung cell models to high levels of aircraft engine oil and hydraulic fluid fumes under ALI conditions can reduce TEER and viabilities of the cells, induce cytotoxicity, and increase production of proinflammatory cytokines. Hydraulic fluid fumes are more toxic than engine oil fumes on the mass concentration of fume basis, which may be related to higher abundance of OPs and smaller particle size of hydraulic fluid fumes. The toxicological data clearly reflect the potential health risks during fume events in aircraft cabins.

 

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2. Jun. 2021

Development of a standardized in vitro approach to evaluate microphysical, chemical, and toxicological properties of combustion-derived fine and ultrafine particles

https://doi.org/10.1016/j.jes.2021.06.001

Ana Teresa Juarez-Facio 1, Clément Castilla 2, Cécile Corbière 1, Hélène Lavanant 2, Carlos Afonso 2, Christophe Morin 2, Nadine Merlet-Machour 2, Laurence Chevalier 3, Jean-Marie Vaugeois 1, Jérôme Yon 4, Christelle Monteil 1,
1Normandie Univ, UNIROUEN, UNICAEN ABTE, 76000 Rouen, France
2Normandie Univ, UNIROUEN, INSA Rouen, CNRS, COBRA, 76000 Rouen, France
3Normandie Univ, UNIROUEN, INSA Rouen, CNRS, GPM-UMR6634, 76000 Rouen, France
4Normandie Univ, UNIROUEN, INSA Rouen, CNRS, CORIA, 76000 Rouen, France

 

The present study illustrate a methodological approach which allows comparing, in an original and innovative way, the physical, chemical, and toxicological properties of different fine and ultrafine particles. In this study, two different operating conditions were used to obtain model particles containing either high (CAST3) or low (CAST1) organic contents, and exposed differentiated NHBE cells “on-line” at the air-liquid interface to be closer to realistic conditions.

 

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3. Mar. 2021

Vitrocell® Leak Tester

Must-have device for aerosol research – easy and efficient leak detection

Regular leak tests are mandatory in aerosol research. A leak caused by a forgotten connection or defective o-ring may have a significant influence on the aerosol exposure process. We recommend to carry out a leak test prior to the experiment and as part of cleaning or service routine.

VITROCELL Application Note

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14. Jan. 2021

Toxic effects of gunshot fumes from different ammunitions for small arms on lung cells exposed at the air liquid interface

https://doi.org/10.1016/j.tiv.2021.105095


Espen Mariussen a,b, Lise Fjellsbø a,c, Tomas Roll Frømyr b, Ida Vaa Johnsen b, Tove Engen Karsrud b, Øyvind Albert Voie b
a Norwegian Institute for Air Research (NILU), PO Box 100, NO-2027 Kjeller, Norway
b Norwegian Defence Research Establishment (FFI), PO Box 25, NO-2027 Kjeller, Norway
c Norwegian University of Life Sciences (NMBU), PO Box 5003, NO-1432 Ås, Norway

 

Highlights
•Gunshot fumes are cytotoxic and induce DNA-damage in lung cells exposed at the air liquid interface.
•Shooting from small arms generate fumes with substantial amounts of nanosized materials.
•Toxicity of the gunshot fumes differed between different types of ammunition.

 

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29. Dec. 2020

Air–Liquid Interface Exposure of Lung Epithelial Cells to Low Doses of Nanoparticles to Assess Pulmonary Adverse Effects

https://doi.org/10.3390/nano11010065

 

Silvia Diabaté 1, Lucie Armand 2, Sivakumar Murugadoss 1 , Marco Dilger 1 , Susanne Fritsch-Decker 1, Christoph Schlager 3, David Béal 2, Marie-Edith Arnal 2, Mathilde Biola-Clier 2, Selina Ambrose 4, Sonja Mülhopt 3, Hanns-Rudolf Paur 3, Iseult Lynch 5 , Eugenia Valsami-Jones 5 , Marie Carriere 2, and Carsten Weiss 1

1 Karlsruhe Institute of Technology, Institute of Biological and Chemical Systems–Biological Information Processing, 76344 Eggenstein-Leopoldshafen, Germany;
2 CEA, CNRS, IRIG, SyMMES, University Grenoble Alpes, 38054 Grenoble, France;
3 Karlsruhe Institute of Technology, Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, Germany; 
4 Promethean Particles Ltd., Nottingham NG7 3EF, UK;
5 School of Geography Earth & Environmental Sciences (GEES), University of Birmingham (UoB), Edgbaston, Birmingham B15 2TT, UK;

 

KIT, together with VITROCELL SYSTEMS, set up a first Automated Exposure Station, which has been used for the assessment of nanoscale particle emissions from combustion sources such as ship diesel and wood burners. The system was further developed and offers a compact solution for toxicity testing of nanoparticle (NP) aerosols including sample conditioning, reproducible deposition, integrated dose determination by a quartz crystal microbalance (QCM), flow control, automated processes and data acquisition. The device was also tested with partner laboratories with the aim of potentially standardizing and achieving regulatory acceptance of the method.

 

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