DOI: 10.1089/aivt.2018.0001
Holger Behrsing,¹ Mario Aragon,¹ Jason Adamson,² Devin Sheehan,¹ Marianna Gaca,² Rodger Curren,¹ and Erin Hill^1
1Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
²R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

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Dr. Dirk Brossell

Federal Institute for Occupational Safety and Health (BAuA), Unit 4.5, 10317 Berlin, Germany

The poster demonstrates the particle deposition via thermal precipitation (particle precipitation by thermophoresis) on cells hosted at the air-liquid interface (ALI).
Advantages of thermophoresis
• Diffusion-like mechanism, gentle particle deposition 
• Independent from particle size (for nanoparticles)
• Homogenous deposition density profile 
• No need to manipulate aerosol properties for deposition (charge, size)

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https://doi.org/10.1016/j.tiv.2018.01.010
E. Bishop, L. Haswell, J. Adamson, S. Costigan, D. Thorne, M. Gaca
British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK

A reconstituted 3D human airway epithelium was exposed to undiluted aerosols at the air-liquid interface, using a Vitrocell VC 10 under ISO and HCI conditions. A 3R4F reference cigarette and a commercially available e-cigarette product were tested on their effect on CBF and TEER.

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Wanda Fields ^a, Kathy Fowler ^a, Victoria Hargreaves ^b, Lesley Reeve ^b, Betsy Bombick ^a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street,Winston-Salem, NC 27101, USA
^b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG1 3PY, UK

This study discribes the development, validation and application of the NRU assay in Chinese Hamster Ovary (CHO) cells, following exposure to fresh whole smoke generated with the VITROCELL®VC10®system. The VITROCELL® VC10® successfully provided consistent generation and delivery of whole smoke from a cigarette (3R4F) and a primarily “heat-not-burn” cigarette (Eclipse).

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DOI 10.1186/s12989-016-0171-3

Thomas Loret^1,2, Emmanuel Peyret¹, Marielle Dubreuil¹, Olivier Aguerre-Chariol³, Christophe Bressot³, Olivier le Bihan³, Tanguy Amodeo³, Bénédicte Trouiller¹, Anne Braun¹, Christophe Egles^2,4 and Ghislaine Lacroix¹

¹Institut National de l’Environnement Industriel et des Risques (INERIS), (DRC/VIVA/TOXI), Parc Technologique ALATA—BP 2, Verneuil-en-Halatte F-60550, France.
²Laboratoire BioMécanique et BioIngénierie (BMBI), Université de Technologie de Compiègne (UTC), UMR CNRS 7338, Compiègne 60205, France.
³Institut National de l’Environnement Industriel et des Risques (INERIS), (DRC/CARA/NOVA), Parc Technologique ALATA—BP 2, Verneuil-en-Halatte F-60550, France.
⁴Department of Biomedical Engineering, Tufts University, Medford, MA, USA.
 

A549 alveolar cells in monocultures and in co-cultures with THP-1 macrophages were exposed to aerosols of inhalable and poorly soluble nanomaterials. The results are evaluated by using the Alamar blue®, LDH, Dichlorofluorescein (DCF) and ELISA Assay and shows that the air-liquid interface represents a valid and sensitive method to assess the toxicity of several poorly soluble nanomaterials.

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