Chem. Res. Toxicol.2016, 29, 1252−1269
Filippo Zanetti,† Alain Sewer,† Carole Mathis,† Anita R. Iskandar,† Radina Kostadinova,† Walter K. Schlage,‡ Patrice Leroy,† Shoaib Majeed,† Emmanuel Guedj,† Keyur Trivedi,† Florian Martin,† Ashraf Elamin,† Celine Merg,† Nikolai V. Ivanov,† Stefan Frentzel,† Manuel C. Peitsch,† and Julia Hoeng*,†
† Philip Morris International Research and Development, Quai Jeanrenaud 5, 2000 Neuchatel, Switzerland
‡ Biology Consultant, Max-Baermann-Str. 21, 51429 Bergisch Gladbach, Germany
The tobacco heating system (THS) 2.2 and the 3R4F reference cigarette smoke were exposed to human organotypic oral epithelial tissue cultures (EpiOral, MatTek)at the Vitrocell 24/48 exposure system. The results are shown by the evaluation of cytotoxicity and cytochrome P450 activity assays, mRNA and miRNA purification, measurements of secreted proin flammatory markers and histopathological analysis.