Anita R. Iskandar, Carole Mathis, Florian Martin, Patrice Leroy, Alain Sewer, Shoaib Majeed, Diana Kuehn, Keyur Trivedi, Davide Grandolfo, Maciej Cabanski, Emmanuel Guedj, Celine Merg, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch and Julia Hoeng
Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland
Human nasal epithelium is the first barrier against inhaled toxicants. The air-liquid interface human nasal culture MucilAir™ offers a more physiologically relevant and robust systems for studying the effects of exposure. This cells were exposed to 3R4F cigarettes and THS2.2 tobacco sticks
in the the Vitrocell® 24/48 exposure system. Results have been evaluated by measuring the cytotoxicity, histology processing, immunostaining, cilia beating frequency (CBF), cytochrome P450 (CYP) activity, luminex-based measurement of secreted analytes, RNA/miRNA purification and mRNA microarray.