Advanced in vitro exposure systems.

7. Jul. 2016

3-D Nasal Cultures: Systems Toxicological Assessment of a Candidate Modified-Risk Tobacco Product

doi: 10.14573/altex.1605041

Anita R. Iskandar, Carole Mathis, Florian Martin, Patrice Leroy, Alain Sewer, Shoaib Majeed, Diana Kuehn, Keyur Trivedi, Davide Grandolfo, Maciej Cabanski, Emmanuel Guedj, Celine Merg, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch and Julia Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

Human nasal epithelium is the first barrier against inhaled toxicants. The air-liquid interface human nasal culture MucilAir™ offers a more physiologically relevant and robust systems for studying the effects of exposure. This cells were exposed to 3R4F cigarettes and THS2.2 tobacco sticks
in the the Vitrocell® 24/48 exposure system. Results have been evaluated by measuring the cytotoxicity, histology processing, immunostaining, cilia beating frequency (CBF), cytochrome P450 (CYP) activity, luminex-based measurement of secreted analytes, RNA/miRNA purification and mRNA microarray.

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12. Feb. 2015

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

New video protocol Exposure of 3D cell cultures using VITROCELL® 24/48 system

jove, 12 Februray 2015, Issue 96 (doi: 10.3791/52325)

Authors
Diana Kuehn1, Shoaib Majeed1, Emmanuel Guedj1, Remi Dulize1, Karine Baumer1, Anita Iskandar1, Stephanie Boue1, Florian Martin1, Radina Kostadinova1, Carole Mathis1, Nikolai V. Ivanov1, Stefan Frentzel1, Julia Hoeng1, Manuel C. Peitsch1

1 Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A

The aim of this video protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke at the air-liquid interface to measure ciliary beating and TEER.

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12. Nov. 2014

Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke

Chemistry Central Journal 2014, 8:62

doi:10.1186/s13065-014-0062-3

Authors
Shoaib Majeed1, Stefan Frentzel1, Sandra Wagner2, Diana Kuehn1, Patrice Leroy1, Philippe A. Guy1, Arno Knorr1, Julia Hoeng1 and Manuel C. Peitsch1

1 Philip Morris Research and Development, Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
2 Eurofins Umwelt West GmbH, Vorgebirgsstraße20, D-50389 Wesseling, Germany

 

The connection of a VITROCELL® 24/48 system to a 30-port carousel smoking machine is shown. The exposure is to verify the cigarette smoke aerosol distribution, the utility of VITROCELL® crystal quartz microbalances and the amount of particles deposited per surface area. The results are shown in cell viability and WST-1 oxidation.

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8. Oct. 2014

The Response of Human Nasal and Bronchial Organotypic Tissue Cultures to Repeated Whole Cigarette Smoke Exposure

International Journal of Toxicology (October 8, 2014)

Authors
Marja Talikka1, Radina Kostadinova1, Yang Xiang1, Carole Mathis1, Alain Sewer1, Shoaib Majeed1, Diana Kuehn1, Stefan Frentzel1, Celine Merg1, Marcel Geertz1, Florian Martin1, Nikolai V. Ivanov1, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

 

Whole Smoke Exposure on nasal and bronchial cells using a VITROCELL®24 system in comparison to animal testings.

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21. Jul. 2014

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

informa healthcare

informa healthcare, posted online on July 21, 2014. (doi:10.3109/15376516.2014.943441)

Authors
Walter K. Schlage*, Anita R. Iskandar*, Radina Kostadinova*, Yang Xiang*, Alain Sewer*, Shoaib Majeed, Diana Kuehn, Stefan Frentzel, Marja Talikka, Marcel Geertz, Carole Mathis, Nikolai Ivanov, Julia Hoeng, and Manuel C. Peitsch

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

*Equal contribution

Exposition of cigarette smoke to tissue cultures at the air–liquid interface shows in combination of the microarray technology, gene-set analysis and a novel computational modeling an increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity. The usefulness of in vitro exposure to buccal and gingival organotypic epithelial tissue cultures for the impact assessment of cigarette smoke is demonstrated. 

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6. Sep. 2013

Comet assay and air-liquid interface exposure system: A new combination to evaluate genotoxic effects of cigarette whole smoke in human lung cell lines

Toxicology in Vitro

Volume 27, Issue 6, September 2013, Pages 1987-1991. pii: S0887-2333(13)00168-9. doi: 10.1016/j.tiv.2013.06.016. [Epub ahead of print]

Authors
Weber S.1, Hebestreit M.1,3, Conroy L.L.1, Rodrigo G.2

1 Philip Morris International R&D, Philip Morris Research Laboratories GmbH, Fuggerstr.3, 51149 Cologne, Germany
2 Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
3 Current address: VITROCELL Systems GmbH, Germany

 

Human lung cell lines A549 and BEAS-2B were exposed to diluted whole smoke in the VITROCELL® 24 and DNA damage was evaluated using the comet assay.

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15. Apr. 2013

VITROCELL User Group Meeting

Ascot, United Kingdom

A total of 29 scientists from China, Europe, India, Japan and USA followed the invitation of VITROCELL® Systems to the 2013 User Group Meeting.

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31. Mar. 2013

New on SOT San Antonio

VITROCELL® 24/48 Module

The new exposure system is a benchmark for in vitro research with higher throughput. It offers up to 8 doses @ 6 replicates in the 24-well size.

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25. Jan. 2013

Human bronchial epithelial cells exposed in vitro to cigarette smoke at the air-liquid interface resemble bronchial epithelium from human smokers

American Journal of Physiology Lung Cellular and Molecular Physiology

2013 Apr 1;304(7):L489-503. doi: 10.1152/ajplung.00181.2012. [Epub ahead of print]

Authors
Mathis C, Poussin C, Weisensee D, Gebel S, Hengstermann A, Sewer A, Belcastro V, Xiang Y, Ansari S, Wagner S, Hoeng J, Peitsch M.

Philip Morris International, Neuchatel, Switzerland

 

The exposition of cigarette smoke on Human AIR-100 tissues shows equivalent results in MMP-1 release, gene expression and microRNA profiles to in vivo smokers' tissue.

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10. Mar. 2011

In vitro exposure of organotypical 3D epithelial tissues to cigarette smoke as a potential alternative to rodent inhalation studies

SOT Washington DC, USA

March 06-10, 2011

Authors
Weisensee, D., Kurkowsky, B., Hebestreit, M., Wagner, S. and Schueller J. 

Philip Morris Research Laboratories GmbH, Cologne, Germany, Abstract #998

 

Here we describe an in vitro test system that examines the toxicological effects of single or repeated cigarette smoke exposure on human organo- typically reconstituted 3D epithelial tissues. These tissues were exposed at the air liquid interface to either fresh air or to mainstream smoke from the Reference Cigarette 3R4F in the VITROCELL® system. 

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