Advanced in vitro exposure systems.

1. Sep. 2016

Aerosol Flow in the Vitrocell 24/48 Exposure System: Flow Mixing and Aerosol Coalescence

Applied In Vitro Toxicology. September 2016, Vol. 2, No. 3: 165-174 

Arkadiusz K. Kuczaj,1,2 Markus Nordlund,1 Santhosh Jayaraju,3 Ed Komen,3 Tobias Krebs,4 Manuel C. Peitsch,1 and Julia Hoeng1
1Philip Morris International Research & Development, Philip Morris Products S.A., Neuchâtel, Switzerland.
2Multiscale Modeling & Simulation, Department of Applied Mathematics, University of Twente, Enschede, The Netherlands.
3NRG, Petten, The Netherlands.
4Vitrocell Systems GmbH, Waldkirch, Germany.

In this work, the flow-mixing efficiency of the dilution unit in the Vitrocell® 24/48 (VC24/48) exposure system and the role of thermal coalescence is presented . The dilution unit of the VC24/48 system guarantees efficient and localized mixing/dilution of the aerosol and the thermal coalescence effects do not influence the aerosol droplet size distribution.

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12. Jul. 2016

Systems Toxicology Assessment of the Biological Impact of a Candidate Modified Risk Tobacco Product on Human Organotypic Oral Epithelial Cultures


Chem. Res. Toxicol.2016, 29, 1252−1269

Filippo  Zanetti, Alain  Sewer, Carole  Mathis, Anita  R.  Iskandar, Radina  Kostadinova, Walter K. Schlage, Patrice Leroy, Shoaib Majeed, Emmanuel Guedj, Keyur Trivedi, Florian Martin, Ashraf  Elamin, Celine  Merg, Nikolai  V.  Ivanov, Stefan  Frentzel, Manuel  C.  Peitsch, and  Julia  Hoeng*,

Philip Morris International Research and Development, Quai Jeanrenaud 5, 2000 Neuchatel, Switzerland
Biology Consultant, Max-Baermann-Str. 21, 51429 Bergisch Gladbach, Germany

The tobacco heating system (THS) 2.2 and the 3R4F reference cigarette smoke were exposed to human organotypic oral epithelial tissue cultures (EpiOral, MatTek)at the Vitrocell 24/48 exposure system. The results are shown by the evaluation of cytotoxicity and cytochrome P450 activity assays, mRNA and miRNA purification, measurements of secreted proin flammatory markers and histopathological analysis.


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7. Jul. 2016

3-D Nasal Cultures: Systems Toxicological Assessment of a Candidate Modified-Risk Tobacco Product

doi: 10.14573/altex.1605041

Anita R. Iskandar, Carole Mathis, Florian Martin, Patrice Leroy, Alain Sewer, Shoaib Majeed, Diana Kuehn, Keyur Trivedi, Davide Grandolfo, Maciej Cabanski, Emmanuel Guedj, Celine Merg, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch and Julia Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

Human nasal epithelium is the first barrier against inhaled toxicants. The air-liquid interface human nasal culture MucilAir™ offers a more physiologically relevant and robust systems for studying the effects of exposure. This cells were exposed to 3R4F cigarettes and THS2.2 tobacco sticks
in the the Vitrocell® 24/48 exposure system. Results have been evaluated by measuring the cytotoxicity, histology processing, immunostaining, cilia beating frequency (CBF), cytochrome P450 (CYP) activity, luminex-based measurement of secreted analytes, RNA/miRNA purification and mRNA microarray.

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12. Feb. 2015

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

New video protocol Exposure of 3D cell cultures using VITROCELL® 24/48 system

jove, 12 Februray 2015, Issue 96 (doi: 10.3791/52325)

Diana Kuehn1, Shoaib Majeed1, Emmanuel Guedj1, Remi Dulize1, Karine Baumer1, Anita Iskandar1, Stephanie Boue1, Florian Martin1, Radina Kostadinova1, Carole Mathis1, Nikolai V. Ivanov1, Stefan Frentzel1, Julia Hoeng1, Manuel C. Peitsch1

1 Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A

The aim of this video protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke at the air-liquid interface to measure ciliary beating and TEER.

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12. Nov. 2014

Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke

Chemistry Central Journal 2014, 8:62


Shoaib Majeed1, Stefan Frentzel1, Sandra Wagner2, Diana Kuehn1, Patrice Leroy1, Philippe A. Guy1, Arno Knorr1, Julia Hoeng1 and Manuel C. Peitsch1

1 Philip Morris Research and Development, Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
2 Eurofins Umwelt West GmbH, Vorgebirgsstraße20, D-50389 Wesseling, Germany


The connection of a VITROCELL® 24/48 system to a 30-port carousel smoking machine is shown. The exposure is to verify the cigarette smoke aerosol distribution, the utility of VITROCELL® crystal quartz microbalances and the amount of particles deposited per surface area. The results are shown in cell viability and WST-1 oxidation.

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8. Oct. 2014

The Response of Human Nasal and Bronchial Organotypic Tissue Cultures to Repeated Whole Cigarette Smoke Exposure

International Journal of Toxicology (October 8, 2014)

Marja Talikka1, Radina Kostadinova1, Yang Xiang1, Carole Mathis1, Alain Sewer1, Shoaib Majeed1, Diana Kuehn1, Stefan Frentzel1, Celine Merg1, Marcel Geertz1, Florian Martin1, Nikolai V. Ivanov1, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland


Whole Smoke Exposure on nasal and bronchial cells using a VITROCELL®24 system in comparison to animal testings.

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21. Jul. 2014

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

informa healthcare

informa healthcare, posted online on July 21, 2014. (doi:10.3109/15376516.2014.943441)

Walter K. Schlage*, Anita R. Iskandar*, Radina Kostadinova*, Yang Xiang*, Alain Sewer*, Shoaib Majeed, Diana Kuehn, Stefan Frentzel, Marja Talikka, Marcel Geertz, Carole Mathis, Nikolai Ivanov, Julia Hoeng, and Manuel C. Peitsch

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

*Equal contribution

Exposition of cigarette smoke to tissue cultures at the air–liquid interface shows in combination of the microarray technology, gene-set analysis and a novel computational modeling an increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity. The usefulness of in vitro exposure to buccal and gingival organotypic epithelial tissue cultures for the impact assessment of cigarette smoke is demonstrated. 

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6. Sep. 2013

Comet assay and air-liquid interface exposure system: A new combination to evaluate genotoxic effects of cigarette whole smoke in human lung cell lines

Toxicology in Vitro

Volume 27, Issue 6, September 2013, Pages 1987-1991. pii: S0887-2333(13)00168-9. doi: 10.1016/j.tiv.2013.06.016. [Epub ahead of print]

Weber S.1, Hebestreit M.1,3, Conroy L.L.1, Rodrigo G.2

1 Philip Morris International R&D, Philip Morris Research Laboratories GmbH, Fuggerstr.3, 51149 Cologne, Germany
2 Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
3 Current address: VITROCELL Systems GmbH, Germany


Human lung cell lines A549 and BEAS-2B were exposed to diluted whole smoke in the VITROCELL® 24 and DNA damage was evaluated using the comet assay.

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15. Apr. 2013

VITROCELL User Group Meeting

Ascot, United Kingdom

A total of 29 scientists from China, Europe, India, Japan and USA followed the invitation of VITROCELL® Systems to the 2013 User Group Meeting.

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31. Mar. 2013

New on SOT San Antonio

VITROCELL® 24/48 Module

The new exposure system is a benchmark for in vitro research with higher throughput. It offers up to 8 doses @ 6 replicates in the 24-well size.

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