Advanced in vitro exposure systems.

12. Aug. 2021

Product News 08/2021

VITROCELL® Remote Assist Support

Let us lend you a helping hand via HoloLens

VITROCELL in vitro exposure systems are specifically manufactured according to customer specifications. Our customers in the field of research & development typically have very complex requirements for the system. This is why a VITROCELL technician is required to carry out product training as well as service & support.
We strive to provide rapid responses and problem-solving for VITROCELL users all over the world.

VITROCELL® Remote Assist Support

Read more

Comments (0) Number of views (348)

20. Apr. 2021

An In Vitro Air-Liquid Interface Inhalation Platform for Petroleum Substances and Constituents

DOI: 10.14573/altex.2010211 


Sandra Verstraelen1, An Jacobs1, Jo Van Laer1, Karen Hollanders1, Masha Van Deun2, Diane Bertels2, Rob Brabers1, Hilda Witters1, Sylvie Remy1,3, Lieve Geerts1, Lize Deferme4 and Evelien Frijns1
1VITO NV (Flemish Institute for Technological Research), Unit HEALTH, Mol, Belgium; 
2VITO NV, Unit Separation and Conversion Technology (SCT), Mol, Belgium; 
3University of Antwerp, Department of Biomedical Sciences, Antwerp, Belgium; 
4ExxonMobil Petroleum and Chemical BV, Machelen, Belgium
 

This pilot study exposed a frequently used in vitro model (A549 cells) at the ALI to assess inhalation toxicity of the single compound EB. Experimental conditions using the VITROCELL® 24/48 exposure system were optimized to achieve a (low) delivery efficiency that resulted in dose-dependent biological changes. The data demonstrate consistency in effect levels when comparing cell viability in the ALI experiments with known in vivo non-lethal effects in humans. It can be concluded that QIVIVE from in vitro air concentrations applied for testing cell viability to in vivo air concentrations may be a promising method for screening for acute inhalation toxicity. 

 

Read more

Comments (0) Number of views (214)

3. Mar. 2021

Vitrocell® Leak Tester

Must-have device for aerosol research – easy and efficient leak detection

Regular leak tests are mandatory in aerosol research. A leak caused by a forgotten connection or defective o-ring may have a significant influence on the aerosol exposure process. We recommend to carry out a leak test prior to the experiment and as part of cleaning or service routine.

VITROCELL Application Note

Read more

Comments (0) Number of views (1317)

18. Nov. 2020

Comparison of the biological impact of aerosol of e-vapor device with MESH® technology and cigarette smoke on human bronchial and alveolar cultures

https://doi.org/10.1016/j.toxlet.2020.11.006

Albert Giralta, Anita R Iskandara, FlorianMartina, ElisaMoschinib, TomassoSerchib, AthanasiosKondylisa, DiegoMarescottia, PatriceLeroya, LauraOrtega-Torresa, ShoaibMajeeda, CelineMerga, KeyurTrivedia, EmmanuelGuedja,StefanFrentzela, Nikolai V Ivanova, Manuel C Peitscha, Arno C Gutlebb, JuliaHoenga


a Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland
b Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Luxembourg

 

Highlights
• Biological impact of exposure to an aerosol of an e-vapor device was investigated using human bronchial and alveolar models.
• The biological impact of e-vapor aerosol exposure was lower than that of cigarette smoke at similar nicotine levels.
• The exposure effects at the molecular levels were evaluated using a systems toxicology approach.

 

Read more

Comments (0) Number of views (187)

8. Oct. 2020

Alternative air–liquid interface method for inhalation toxicity testing of a petroleum-derived substance

https://doi.org/10.1016/j.mex.2020.101088


Verstraelen Sandra a , Jacobs An a , Van Laer Jo a , Van Deun Masha b , Bertels Diane b , Hilda Witters a , Remy Sylvie a , c , Geerts Lieve a , Deferme Lize d , Frijns Evelien a
a VITO NV (Flemish Institute for Technological Research), Unit HEALTH, Mol, Belgium 
b VITO NV, Unit SCT (Separation and Conversion Technology), Mol, Belgium 
c Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium 
d ExxonMobil Petroleum and Chemical B.V., Machelen, Belgium

 

A549 cells were exposed to gasoline at the ALI by passive dosing, an approach that is already used in aquatic toxicity testing. This showes a clear dose-dependent biological response. This in vitro -based new approach methodology might be promising for inhalation toxicity testing of (semi-)volatile complex substances.

 

Read more

Comments (0) Number of views (118)

29. Sep. 2020

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

https://doi.org/10.1007/s11626-020-00517-7


Xuefei Cao1, Jayme P. Coyle2, Rui Xiong1, Yiying Wang1, Robert H. Heflich1, Baiping Ren1, William M. Gwinn3, Patrick Hayden4, Liying Rojanasakul2

1 Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Rd., AR Jefferson, USA
2 Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers forDisease Control and Prevention,Morgantown,WV, USA
3 Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Durham, NC, USA
4 BioSurfaces Inc., Ashland, MA, USA


One important element for validating any new assay for making regulatory decisions is determining its performance relative to an accepted standard. Conducting in vivo inhalation toxicity studies using whole-body or nose-only exposure systems is expensive and time-consuming and typically requires a large number of animals. The goal of using alternative methods, like human in vitro ALI airway cultures, ultimately is to replace inhalation toxicity testing in animals with in vitro approaches. Transition from animal- to human-based models is ultimately expected to lead to faster and better predictive toxicity assessments and therapeutic development at lower cost.  This study shows the development and validation of alternative in vitro methods for acute toxicity testing, including acute inhalation toxicity testing.
 

Read more

Comments (0) Number of views (234)

30. Nov. 2019

Dosimetric Analysis of Aerosol Generated by a Vitrocell® VC10® Smoking Robot – Investigations on Dose Dependency and Consistency of Application


Adam Seymour, Michael Hollings, Joanne Larner and Julie Clements;

Covance Laboratories Ltd, Harrogate, UK


QCMs were used to assess the deposition of cigarette aerosol from a Vitrocell® VC10® in Vitrocell® Ames, 6/4, 12/4 and 24/4 exposure modules. The power to resolve difference between 8 and 1 SLPM is statistically significant in all module types assessed. Uniformity within each module was confirmed (P >0.98) in all module types, at all airflows. Repeatability was assessed and found to be consistent (no significant difference) in all module types at airflows of 10, 8 and 4 SLPM, however, at 1 SLPM a significant difference was noted in 9 out of 12 experiments – likely an artifact of high deposition.
These results show that exposures utilizing the Vitrocell® VC10® and associated modules are robust; but indicate the necessity of dosimetry measures to aid in the quantification of delivered dose.
 

Read more

Comments (0) Number of views (133)

27. Nov. 2019

State-of-the-art methods and devices for the generation, exposure, and collection of aerosols from heat-notburn tobacco products

Stéphanie Boué1, Didier Goedertier1, Julia Hoeng1 , Arkadiusz Kuczaj1, Shoaib Majeed1, Carole Mathis1, Anne May2 , Blaine Phillips3, Manuel C Peitsch1, Falk Radtke1, Walter K Schlage4, Wei Teck Tan3 and Patrick Vanscheeuwijck1

1 Philip Morris International (PMI) Research & Development, Philip Morris Products S.A., Neuchâtel, Switzerland
2 Consultants in Science, Epalinges, Switzerland
3 Philip Morris International (PMI) Research & Development, Philip Morris International Research Laboratories Pte. Ltd, Science Park II, Singapore
4 Biology Consultant, Bergisch Gladbach, Germany

 

The VC 24/48 exposure system is being validated for the exposure process of three-dimensional, organotypic cell culture inserts with CS and with aerosols generated from HNB tobacco products and e-liquids.
They aerosol deposition of different CS concentrations as determined by three different approaches were assessed and compared : (1) a WST-1 colorimetric assay; (2) the determination of eight carbonyls trapped in PBS; and (3) QCM-determined particle mass deposition.

 

Read more

Comments (0) Number of views (1442)

31. Oct. 2019

Deposition efficiency and uniformity of monodisperse solid particle deposition in the Vitrocell® 24/48 Air–Liquid-Interface in vitro exposure system

Aerosol Science and Technology

DOI: 10.1080/02786826.2019.1676877

Michael J. Oldhama , Nicolas Castroa, Jingjie Zhanga, Ali Rostamia, Francesco Luccib, Yezdi Pithawallaa,
Arkadiusz K. Kuczajb,c , I. Gene Gilmand, Pasha Kosachevskye, Julia Hoengb, and K. Monica Leea
aAltria Client Services, Richmond, Virginia, USA; 
bPhilip Morris International Research & Development, Philip Morris Products S.A. (part of Philip Morris International group of companies), Neuchatel, Switzerland; 
cMultiscale Modeling & Simulation, Department of Applied Mathematics, University of Twente, AE Enschede, The Netherlands; 
dEnthalpy Analytical, Durham, North Carolina, USA; eEnthalpy Analytical, Richmond, Virginia, USA

 

Monodispersed solid particles were used to investigate experimental measurement of deposition efficiency, variability of deposition efficiency within and between rows and uniformity of deposition across all cell culture inserts of the Vitrocell 24/48 ALI in vitro exposure system.

 

Read more

Comments (0) Number of views (1517)

7. Sep. 2019

Application of a multi‑layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e‑liquid and its corresponding aerosol using an e‑cigarette device

https://doi.org/10.1007/s00204-019-02565-9


Anita R. Iskandar, Filippo Zanetti, Diego Marescotti, Bjorn Titz, Alain Sewer, Athanasios Kondylis, Patrice Leroy, Vincenzo Belcastro, Laura Ortega Torres, Stefano Acali, Shoaib Majeed, Sandro Steiner, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Thomas Schneider, Stefan Frentzel, Florian Martin, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng


Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

Previous experimental setups shows the effects of e-liquids on cell viability (first layer), followed by investigating the potential mechanisms of toxicity elicited by e-liquids (second layer) and finally assessing the impacts of aerosols (third layer). In this present work shows how the three-layer framework is leveraged to evaluate the potential toxicity and biological effects of the MESH Classic Tobacco and Base e-liquids/aerosols compared with those of 3R4F CS.

 

Read more

Comments (0) Number of views (1977)
RSS
1234
Back to Top