Advanced in vitro exposure systems.

28. Oct. 2018

Big tobacco embraces in vitro toxicology

Manufacturers invest in nonanimal tests to evaluate health risks of e-cigarettes and other next-generation tobacco products

c&en, Volume 96, Issue 43

Britt E. Erickson

The complexity of next-generation tobacco products has increased by a number of factors since FDA was given responsibility to regulate tobacco. Testing e-liquids with animals is not practical, economically and not that accurate. The Vitrocell system generates airborne materials from test products, such as smoke from reference cigarettes and vapor from e-cigarettes, in a manner that simulates how a person would puff. This artcile shows, how IIVS scientists use human donor tissues, obtained from organs, to create cell cultures and in vitro test systems.


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30. Jun. 2018

Characterization and modeling of aerosol deposition in Vitrocell® exposure systems - exposure well chamber deposition efficiency

DOI 10.1016/j.jaerosci.2018.06.015

 

Francesco Luccia, Nicolas D. Castrob, Ali A. Rostamib, Michael J. Oldhamb, Julia Hoenga, Yezdi B. Pithawallab, Arkadiusz K. Kuczajac

a Philip Morris International Research & Development, Philip Morris Products S.A. (part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland
b Altria Client Services LLC, Center for Research and Technology, Richmond, VA 23219, USA
c Multiscale Modeling & Simulation, Dept. of Applied Mathematics, University of Twente, P.O.Box 217, 7500 AE Enschede, The Netherlands

This paper successfully presents the geometry and characteristic dimensions of the two exposure systems: Vitrocell 24/48 and Vitrocell AMES 48. A computational modeling of aerosol transport and deposition shows the deposition efficiency.


 

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11. Mar. 2018

VITROCELL® 6/48: Changeover set for 24-well sized inserts

User Group Meeting 2018

Tobias Krebs

VITROCELL Systems GmbH, 79183 Waldkirch, Germany 

 

The poster shows the VITROCEL® 48 exposure system with a 6- and a 24-well setup.

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11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

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1. Mar. 2018

Comparative biological impacts of an aerosol from carbon-heated tobacco and smoke from cigarettes on human respiratory epithelial cultures: A systems toxicology assessment

https://doi.org/10.1016/j.fct.2018.02.063
Anita R. Iskandar, Florian Martin, Patrice Leroy, Walter K. Schlage, Carole Mathis, Bjorn Titz, Athanasios Kondylis, Thomas Schneider, Grégory Vuillaume, Alain Sewer, Emmanuel Guedj, Keyur Trivedi, Ashraf Elamin, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
PMI R&D, Philip Morris Products S.A., Part of Philip Morris International group of companies, Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland


Highlights
• Aerosols of a heat-not-burn tobacco product elicited lower impacts compared with smoke of 3R4F reference cigarettes.
• Systems toxicology revealed changes at the cellular–molecular levels not otherwise apparent from morphological features.
• The reduced impact of heated tobacco aerosols on small airway was similar than that on nasal epithelial cultures.

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1. Feb. 2018

Comparative Effect of a Candidate Modified Risk Tobacco Product Aerosol and Cigarette Smoke on Color Stability of Enamel, Dentin and Dental Composite Resins

SRNT 2018

Filippo Zanetti1, Xiaoyi Zhao2,3, Shoaib Majeed1, Hans Malmstrom2, Manuel C. Peitsch1, Yanfang Ren2, Julia Hoeng1

1 PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland (part of Philip Morris International group of companies)
2
University of Rochester Eastman Institute for Oral Health
, Rochester, New York
3
Peking University School of Stomatology
, Peking, China

In this study, the effects of 3R4F cigarette smoke and THS2.2 aerosol on color stability and surface roughness of premolar human teeth were investigated.

The poster was presented at SRNT 2018, https://www.srnt.org/

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17. Sep. 2017

Integration of Targeted Proteomics into Systems Toxicology Approach: Candidate Modified-Risk Tobacco Product Assessment using Nasal Cultures

HUPO 2017, Dublin

T. Schneider, A. Elamin, A.R. Iskandar, F. Zanetti, C. Merg, A. Sewer, B. Titz, P. Leroy, E. Guedj, S. Majeed, L. Ortega-Torres, W. Schlage, C. Mathis, F. Martin, S. Frentzel, N. V. Ivanov, M.C. Peitsch, J. Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland (Part of Philip Morris International group of companies)

The objectives of this study included to demonstrate robustness and reproducibility of systems biology data obtained from an organotypic in vitro nasal culture model for cigarette smoke and aerosol exposure assessment. Cellular stress and pro-inflammatory responses were investigated on the primary nasal epithelial cells of a non-smoker.

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10. Sep. 2017

On-Line Aerosol Characterization within Exposure Systems Using Soft Ionization Time of Flight Mass Spectroscopy

Eurotox 2017

Sandro Steiner, Shoaib Majeed, Arkadiusz Kuczaj, Julia Hoeng, Stefan Frentzel
Philip Morris International R&D, Philip Morris Products S.A. (part of Philip Morris International group of companies)

Establishment of a setup for sampling and analyzing complex aerosols on-line during exposures with the Vitrocell 24/48 aerosol exposure system.
The poster was presented at Eurotox 2017, http://www.eurotox2017.com

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10. Sep. 2017

Systems toxicology assessment of repeated exposure to cigarette smoke and a candidate modified-risk tobacco product aerosol on gingival organotypic cultures

Eurotox 2017

Filippo Zanetti, Alain Sewer, Patrice Leroy, Shoaib Majeed, Laura Ortega Torres, Céline Merg, Maica Corciulo, Keyur Trivedi, Emmanuel Guedj, Ashraf Elamin, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
Philip Morris International Research and Development, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

The objective of this study was to assess and compare the effects of repeated exposures to the aerosol of CHTP1.2 with cigarette smoke generated from reference cigarettes (3R4F) on human gingival organotypic epithelial cultures. The results were evaluated in using Adenylate Kinase (AK)-based cytotoxicity assay, histological analysis, pro-inflammatory mediators and Microarray data processing and analysis.

The poster was presented at Eurotox 2017, http://www.eurotox2017.com

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20. Aug. 2017

In Vitro Systems Toxicology Assessment of Exposure to Aerosol from a Carbon Heated Tobacco Product as Compared with Exposure to Cigarette Smoke: The Impact on Nasal and Small Airway Epithelial Culture

The 10th World Congress on Alternatives and Animal Use in the Life Sciences, Seattle, Washington, USA

Anita R. Iskandar, Yannick Martinez, Carole Mathis, Patrice Leroy, Florian Martin, Alan Sewer, Laura Ortega Torres, Shoaib Majeed, Keyur Trivedi, Stefan Frentzel, Emmanuel Guedj, Celine Merg, Ashraf Elamin, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland (part of Philip Morris International group of companies)

The aim of the study is the characterization of the 3R4F smoke and CHTP1.2 aerosol in the Vitrocell 24/48® exposure system including the measurements of nicotine in the diluted 3R4F smoke and THS2.2 aerosol. Cytotoxicity was assessed, concentrations of pro-inflammatory mediators were measured, mRNA microarrays and the histological analysis were done.

The poster was presented at the 10th World Congress on Alternatives and Animal Use in the Life Sciences, Seattle, Washington, USA.

http://wc10seattle.org/2017/home.aspx

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