Sonja Mülhopt¹, Christoph Schlager², Markus Berger², Sivakumar Murugadoss³, Peter H. Hoet³, Tobias Krebs², Hanns-Rudolf paur¹ & Dieter Stapf^1
1Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, Eggenstein-Leopoldshafen, 76344, Germany. 
²Vitrocell Systems GmbH, Waldkirch, 79183, Germany. 
³KU Leuven, Environment and Health, Leuven, 3000, Belgium.

The surface dose and the spatial distribution on the membrane delivers important data for  measuring dose-response relationships in toxicity studies.  Image evaluation of transmission electron  microscopy (TEM) samples is a highly sensitive method for determination of deposition. This paper reports  the development and characterization of a novel holder for film coated TEM copper grids, which allows for  sampling under identical geometric and ambient conditions as in a cell culture chamber. 

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https://doi.org/10.1016/j.mrgentox.2020.503135

Authors
StefanPfuhler^a, Janvan Benthem^b, RodgerCurren^c, Shareen H.Doak^d, MariaDusinska^e, MakotoHayashi^f, Robert H.Heflich^g, DarrenKidd^h, DavidKirklandⁱ, YangLuan^j, GladysOuedraogo^k, KerstinReisinger^l, ToshioSofuni^m, Frédériquevan Ackerⁿ, YingYang^o, RaffaellaCorvi^p
a Procter and Gamble, Mason Business Centre, Mason, OH, USA
^b National Institute for Public Health and the Environment, Centre for Health Protection, Bilthoven, the Netherlands
^c Institute for In Vitro Sciences, Inc., Gaithersburg, MD, USA
^d Swansea University Medical School, Singleton Park, Swansea, SA2 8PP, Wales, UK
^e Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute for Air Research, Kjeller, Norway
^f makoto international consulting, Ebina, Japan
^g U.S. Food and Drug Administration/National Center for Toxicological Research, Jefferson, AR, USA
^h Covance Laboratories Ltd, Otley Road, Harrogate, HG3 1PY, UK
ⁱ Kirkland Consulting, PO Box 79, Tadcaster, LS24 0AS, UK
^j School of Public Health, Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, PR China
^k L’Oréal R&I, Aulnay-sous-bois, France
^l Henkel AG & Co KGaA, Duesseldorf, Germany
^m Formerly National Institute of Health Sciences, Tokyo, Japan
ⁿ Triskelion B.V., Zeist, the Netherlands
^o Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, PR China
^p European Commission, Joint Research Centre (JRC), Ispra, Italy

Highlights
• Extensive progress made in development of 3D organ-based genotoxicity assays.
• 3D culture models represent major exposure routes: dermal, oral, inhalation.
• The 3D skin comet and MN assays are considered mature and sufficiently validated.
• Liver and airway model-based genotoxicity assays show promise but are at early stage.<

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Oliver Wisser¹, Bastian Gutmann¹, Adam Seymour^2
1VITROCELL Systems GmbH 79183 Waldkirch, Germany
²Covance Laboratories Ltd Harrogate, UK

The poster shows the new media inserts.

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Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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https://doi.org/10.1016/j.yrtph.2018.05.004

Shinkichi Ishikawa∗, Kazushi Matsumura, Nobumasa Kitamura, Kanae Ishimori, Yuichiro Takanami, Shigeaki Ito

The experimental setup for the aerosol exposure consisted of a VC 10 smoking robot, a dilution system, and a Vitrocell 12/3 glass module the exposure of nicotine and TPM in the mainstream aerosols from 3R4F and novel tobacco vapor on MucilAir™ cells. The results are shown in cytotoxicity, IL-8, IL-1ß and gene expression.

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