The Quartz Crystal Microbalance is a powerful tool for dosimetry in VITROCELL Exposure Systems. However, interpreting live-data from a sQCM can be misleading, as there is a lot going on in parallel during measurements.

VITROCELL Application Note

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https://doi.org/10.1172/jci.insight.155589

Kristine M. Abo,^1,2 Julio Sainz de Aja,^3,4,5 Jonathan Lindstrom-Vautrin,¹ Konstantinos-Dionysios Alysandratos,^1,2 Alexsia Richards,⁶ Carolina Garcia-de-Alba,^3,4,5 Jessie Huang,^1,2 Olivia T. Hix,^1,2 Rhiannon B. Werder,^1,2 Esther Bullitt,⁷ Anne Hinds,² Isaac Falconer,⁸ Carlos Villacorta-Martin,¹ Rudolf Jaenisch,^6,9 Carla F. Kim,^3,4,5 Darrell N. Kotton,^1,2 and Andrew A. Wilson^1,2
1Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA. 
²The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA.
³Stem Cell Program and Divisions of Hematology/Oncology and Pulmonary & Respiratory Diseases, Boston Children’s Hospital, Boston, Massachusetts, USA. 
⁴Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. 
⁵Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. 
⁶Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA. 
⁷Department of Physiology & Biophysics, Boston University, Boston, Massachusetts, USA. 
⁸Boston University School of Medicine, Boston, Massachusetts, USA. 
⁹Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

This study comprehensively characterize the effects of ALI culture on iAT2s and benchmark their transcriptional profile relative to both freshly sorted and cultured primary human fetal and adult AT2s. They find that iAT2s cultured at ALI maintain an AT2 phenotype while upregulating expression of transcripts associated with AT2 maturation. Then they leverage this platform to assay the effects of exposure to clinically significant, inhaled toxicants including cigarette smoke and electronic cigarette vapor.

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Let us lend you a helping hand via HoloLens

VITROCELL in vitro exposure systems are specifically manufactured according to customer specifications. Our customers in the field of research & development typically have very complex requirements for the system. This is why a VITROCELL technician is required to carry out product training as well as service & support.
We strive to provide rapid responses and problem-solving for VITROCELL users all over the world.

VITROCELL® Remote Assist Support  (PDF)

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Regular leak tests are mandatory in aerosol research. A leak caused by a forgotten connection or defective o-ring may have a significant influence on the aerosol exposure process. We recommend to carry out a leak test prior to the experiment and as part of cleaning or service routine.

VITROCELL Application Note

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doi:10.3390/nano10122369

Lars B. Leibrock ¹, Harald Jungnickel ¹, Jutta Tentschert ¹, Aaron Katz ¹, Blaza Toman ² , Elijah J. Petersen ³ , Frank S. Bierkandt ¹, Ajay Vikram Singh ¹ , Peter Laux ¹ and Andreas Luch ^1
1 German Federal Institute for Risk Assessment (BfR), Department of Chemical and Product Safety, Max-Dohrn-Strasse 8-10, 10589 Berlin, Germany; 
² Information Technology Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaitherburg, MD 20899-8311, USA; 
³ Materials Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaitherburg, MD 20899-8311, USA; 

ALI systems are considered to be a promising exposure system to study toxicological e ects of airborne nanomaterials instead of in vivo inhalation studies and have been widely used to assess the toxicology of nanomaterials in recent years. However, the robustness of these methods is not yet wellunderstood. Here we reported a C&E analysis of a commonly used flow through ALI exposure system.

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